대한환경공학회지 (Journal of Korean Society of Environmental Engineers)

  • 제27권11호
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  • Pages.1198-1204
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  • 2005
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  • 1225-5025(pISSN)
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  • 2383-7810(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
권호 표지

무기배지에서 메탄산화균의 분리배양과 COD 생성 및 탈질.탈인 특성 연구

Isolation and Culture of Methanotrophs in Inorganic Medium and Characterization of COD Production, Nutrient Removal

  • 김이태 (한국건설기술연구원 건설환경연구부) ;
  • 배우근 (한양대학교 건설환경시스템공학과) ;
  • 김광수 (한국건설기술연구원 건설환경연구부) ;
  • 이희자 (한국건설기술연구원 건설환경연구부)
  • Kim, I-Tae (Korea Institute of Construction Technology) ;
  • Bae, Woo-Keun (Hanyang University Department of Civil & Environmental Systems Engineering) ;
  • Kim, Kwang-Soo (Korea Institute of Construction Technology) ;
  • Lee, Hee-Ja (Korea Institute of Construction Technology)
  • 발행 : 2005.11.30
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초록

본 연구는 메탄산화균을 분리 배양하여 생물학적 질소 및 인 제거에 응용하는데 있다. 수도권 매립지의 상부 토양으로부터 NMS (nitrate mineral salt solution)배지로 분리 배양된 메탄산화균을 이용하여 영양염 제거 및 성장특성을 분석하였다. 분리 배양된 메탄산화균은 탈질의 탄소원으로서 이용될 수 있는 메탄올, 포름알데히드, 포름산으로 구성된 상당한 양의 유기물(COD 증가)을 생산하였다. 이때 메탄올의 생성속도는 $8\;mg/L{\cdot}hr$로 나타났다. 메탄산화균의 슬러지에 함유되어 있는 질소와 인의 함량을 볼 때 메탄산화균은 탈질에 필요한 탄소원 생성 뿐만 아니라 자체적으로 질소와 인을 성장기질로서 사용하는 것으로 나타났다.

The objectives of this study were to isolate and culture methanotrophs and to apply them for biological removal of nitrogen and phosphorous. Methanotrophs (dominant species: Methylomonas methanica) were isolated from a landfill cover soil, cultured in a NMS medium, and analyzed to reveal their characteristics of growth and nutrient removal. The methanotrophs themselves can produce substantial amount of organic substances(as COD) including methanol, formaldehyde, and formate, as carbon sources required for denitrification. For instance, the production rate for methanol was $8\;mg/L{\cdot}hr$. Moreover, the analysis of nitrogen and phosphorous in the sludge suggested that the methanotrophs assimilate nitrogen and phosphorous as growth substances.

키워드

참고문헌

  1. Water Res. v.31 no.1 The use of $CH_4$ as a sole carbon source for wastewater denitrification Thalasso, F.;Vallecillo, A.;Garcia, D.E.;Polanco, F.F. https://doi.org/10.1016/S0043-1354(96)00228-X
  2. Water Res. v.33 Denitrification with natural gas and various new growth media Pajapakse, J.P.;Scutt, J.E. https://doi.org/10.1016/S0043-1354(99)00088-3
  3. Water Sci. Technol. v.23 Denitrification with biogas as external carbon source Werner, M.;Kayser, R.
  4. J. Gen. Microbiol. v.125 Methane-oxidizing activity and membrane morphology in methanol-grown obligate methanotroph Methylosinus trichosporium OB3b Best, D.J.;Higgins, I.J.
  5. Methane oxidation by methanotrophs: physiological and mechanistic implications;Methane and methanol utilizers Dalton, H.;Murrell, J.C.(ed.);Dalton, H.(ed.)
  6. Food technol. Biotechnol. v.39 no.1 Effect of growth conditions on the expression of soluble methane monooxygenase Ana, B.;Dubravka, H.
  7. Biodegradation v.1 Optimization or trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity Brusseau, G.A.;Tsien, H.C.;Hanson, R.S.;Wackett, L.P. https://doi.org/10.1007/BF00117048
  8. Scand J Infect Dis v.32 Identification of non-tuberculous mycobacteria: 16S rRNA gene sequence analysis vs. conventional methods El Amin, N.M.;Hanson, H.S.;Pettersson, B.; Petrini, B.;Von Stedin, L.V. https://doi.org/10.1080/00365540050164218
  9. Microbiol Rev. v.60 Methanotrophic bacteria Hanson, R.S.;Hanson, T.E.
  10. Appl. Environ. Microbiol. v.66 no.12 Molecular characterization of methanotrophic isolates from freshwater lake sediment Auman, Ann J.;Stobyar, Sergei;Costello, Andria M.;Lidstrom, Mary E. https://doi.org/10.1128/AEM.66.12.5259-5266.2000
  11. Microbial gas metabolism: mechanistic, metabolic and biotechnological aspects Leak, D.J.;Stanley, S.H.;Dalton, H.;Pool, R.K.(ed.);Dow, C.S.(ed.)
  12. Appl. Environ. Microbiol. v.55 no.12 Biodegradation of trichloroethylene by methylosius trichosporium OB3b Tsien, H.C.;Brusseau, G.A.;Hanson, R.S.;Wackett, L.P.
  13. Environ, Sci. Technol. v.23 no.11 Degradation of trichloroethylene and trans-1,2-dichloro-ethylene by a methanotrophic consortium in a fixed film, packed-bed bioreactor Strandberg, G.W.;Donaldson, T.L.;Farr, L.L. https://doi.org/10.1021/es00069a016
  14. Appl. Environ. Microbiol. v.55 no.11 Degradation of chlorinated aliphic hydrocarbon by methylosinus trichosporium OB3b expressing soluble methane monooxygenase Oldenhuis, R.;Vink, L.J.R.;Janssen, D.B.;Witholt, B.
  15. The biochemistry of methylotrophs Anthony, C.
  16. Microbio. Ecolo. v.47 Nitrogen as a regulatory factor of methane oxidation in soils and sediments Bodelier, Paul L.E.;Hendrikus, J.L. https://doi.org/10.1016/S0168-6496(03)00304-0
  17. FEMS Microbiol Letters v.87 no.3-4 Studies on phosphate metabolism in obligate methanotrophs Trotsenko, Y.A.;Shishkina, V.N. https://doi.org/10.1111/j.1574-6968.1990.tb04876.x