Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Effects of Modifiers on the Supercritical $CO_2$ Extraction of Licorice (Glycyrrhiza glabra) and the Morphology of Licorice Tissue

  • Kim, Hyun-Seok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lim, Gio-Bin (Department of Chemical and Bioengineering, The University of Suwon) ;
  • Kim, Byung-Yong (Department of Food Science and Biotechnology, Kyung Hee University)
  • Published : 2005.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Optimal extraction conditions such as pressures, temperatures, and modifiers on glycyrrhizin extraction from licorice were investigated using supercritical $CO_2\;(SC-CO_2)$ at 3 mL/min flow rate. Morphology of licorice tissue, after glycyrrhizin extraction, was examined by SEM, and absolute density ($g/cm^3$) measurement and glycyrrhizin content were determined by HPLC. Pure $SC-CO_2$ had no effect on glycyrrhizin extraction, but recovery of glycyrrhizin ($32.66{\pm}0.77%$) was enhanced when water was used as modifier. The highest recovery was $97.22{\pm}2.17%$ when 70% (v/v) aqueous methanol was added to 15% (v/v) $SC-CO_2$ at 50 MPa and $60^{\circ}C$. Under optimal extraction conditions, 30 MPa pressure and $60^{\circ}C$ heating temperature, glycyrrhizin recovery reached maximum ($102.67{\pm}1.13%$) within 60 min. Licorice tissue was severely damaged by excessive swelling, and absolute density of licorice residues was highest when aqueous methanol was used as a modifier.

Keywords

References

  1. Food Chem. v.38 GlycyrrhiZll glabra L.- composition, uses and analysis Fenwick, G.R.;Lutomski, J.;Liquorice, Nieman C. https://doi.org/10.1016/0308-8146(90)90159-2
  2. J. Sci. Food Agric. v.77 Monoammonium glycyrrhizinate stability in aqueous buffer solutions Celine, A.C.;Laurence, J.C.;Francoise, M.P.;Yannick, M.R.H. https://doi.org/10.1002/(SICI)1097-0010(199808)77:4<566::AID-JSFA84>3.0.CO;2-I
  3. Food Chem. v.70 Foaming behaviour of liquorice (GlycyrrhiZll glabra) extract Esra, I.;Senol, I. https://doi.org/10.1016/S0308-8146(00)00098-4
  4. Int. J. Pharma. v.119 Antiulcer activities of liquorice and its derivatives in experimental gastric lesion induced by ibuprofen in rats Dehpour, A.R.;Zolfaghari, M.E.;Samadian, T.;Kobarfard, F.;Faizi, M.;Assari, M.
  5. Plant Gen. Res. Newslett. v.100 A study on in vitro cultivation of Glcyrrhizia glabra Dimitrova, D.;Varbanova, K.;Paeva, I.;Angelova, S.;Guteva, Y.
  6. Cancer Lett. v.145 Effect of liquorice and glycyrrhizin on rat liver carcinogen metabolizing enzymes Paolini, M.;Barillari, J.;Broccoli, M.;Pozzetti, I.;Perocco, P.;Cantelli-Forti, G. https://doi.org/10.1016/S0304-3835(99)00225-6
  7. Biochem. Eng. J. v.5 Microwave-assisted extraction of glycyrrhizic acid from licorice root Xuejun, P.;Huizhou, I.;Guanghe, J.;Youn, Y.S. https://doi.org/10.1016/S1369-703X(00)00057-7
  8. Food Chem. v.52 Applications for supercritical fluid technology in food processing Palmer, M.Y.;Ting, S.S.T. https://doi.org/10.1016/0308-8146(95)93280-5
  9. Food Technol. v.40 Supercritical fluid extraction: operating principles and food applications Rizvi, S.S.H.;Daniels, J.A.;Benado, A.I.;Zollweg, J.A.
  10. Talanta v.53 Supercritical fluid extraction in herbal and natural product studies-a practical review Qingyong, L.;Chien, M.W. https://doi.org/10.1016/S0039-9140(00)00557-9
  11. Talanta v.46 Supercritical fluid extraction of phenol compounds from olive leaves loch, F.L.;Tena, M.T.;Rios, A.;Valcarcel, M. https://doi.org/10.1016/S0039-9140(97)00375-5
  12. J. Chromatogr. A v.830 Supercritical fluid extraction of flavonoids from Scutellariae Radix Lin, M.C.;Tsai, M.J.;Wen, K.C.
  13. J. Chromatogr. A v.863 Strategies for supercritical fluid extraction of hyoscyamine and scopolamine salts using basified modifiers Choi, Y.R.;Chin, Y.W.;Kim, J.W.;Jeon, S.H.;Yoo, K.P. https://doi.org/10.1016/S0021-9673(99)00962-0
  14. Korean J. Biotech. Bioeng. v.16 Ethanol modified supercritical $CO_2$ extraction of daidzein from soybean Boo, S.J.;Byun, S.Y.
  15. J. Supercrit. Fluids v.22 Supercritical fluid extraction of borage (Borage officinalis L.) seeds with pure $CO_2$ and its mixture with caprylic acid methyl ester Egidijus, D.;Petras, R.Y.;Bjorn, S. https://doi.org/10.1016/S0896-8446(01)00116-4
  16. Food Sci. Biotechnol. v.12 Extraction of carotenoids from Citrus unshiu press cake by supercritical carbon dioxide Lim, S.B.;Jung, S.K.;Jwa, M.K.
  17. Anal. Chem. v.65 Modifier effects in the supercritical fluid extraction of solutes from clay, soil, and plant materials Fahmy, T.M.;Paulaitis, M.E.;Johnson, D.M.;McNally, M.E.P. https://doi.org/10.1021/ac00058a026
  18. J. Chromatogr. A. v.936 Supercritical fluid extraction and chromatography for fat-soluble vitamin analysis Turner, C.;King, J.W.;Mathiasson, L. https://doi.org/10.1016/S0021-9673(01)01082-2
  19. Optimization of extraction of glycyrrhizin from licorice (GlcyyrrhziZll glabra) using supercritical $CO_2$ Kim, H.S.
  20. J. Supercrit. Fluids v.23 Low temperature drying of low rank coal by supercritical carbon dioxide with methanol as entrainer Iwai, Y.;Koujina, Y.;Arai, Y.;Watanabe, I.;Mochina, I.;Sakanishi, K. https://doi.org/10.1016/S0896-8446(02)00005-0
  21. Anal. Chem. v.67 Water solubility measurements in supercritical fluids and high-pressure liquids using nearinfrared spectroscopy Jackson, K.;Bowman, L.E.;Fulton, J.L. https://doi.org/10.1021/ac00110a007
  22. J. Chromatogr. A. v.505 Extraction of major alkaloids from poppy straw with near critical mixtures of carbon dioxide and polar mixtures Janicot, J.L.;Caude, M.;Rosset, R. https://doi.org/10.1016/S0021-9673(01)93083-3
  23. J. Food Eng. v.47 Supercritical COz extraction of nimbin from neem seeds - an experimental study Pathumthip, T.;Supapom, C.;Peter, D.;Wilai, L. https://doi.org/10.1016/S0260-8774(00)00131-X