Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cells by Essential Oil of Chrysanthemum boreale Makino

  • Cha, Jeong-Dan (Major in Food and Nutrition, Wonkwang University) ;
  • Jeong, Mi-Ran (Institute of Oral Bioscience, Department of Oral Microbiology, School of Dentistry, Chonbuk National University) ;
  • Lee, Young-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • Published : 2005.06.30

Abstract

The effect of the essential oil obtained from Chrysanthemum boreale Makino on the apoptosis of KB cells was investigated. Cytotoxicity and cellular DNA content were analyzed by MTT assay, flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. The various cytotoxic effects of the essential oil which are hallmarks of apoptosis, including DNA fragmentation, apoptotic body formation, and sub-G1 DNA content, all progressed in a dose-dependent manner. Treatment with an apoptosis-inducing concentration of the essential oil caused rapid and transient induction of caspase 3 activity. Further, the efficacious induction of PARP cleavage and caspase-3 activation was observed at an essential oil concentration of 0.1 and 0.2 mg/mL for 12 hr.

References

  1. Annu. Rev. Immunol. v.10 Apoptosis and cell death in immunity Cohen, J.J.;Duke, R.C.;Fadok, V.A.;Sellins, K.S. https://doi.org/10.1146/annurev.iy.10.040192.001411
  2. Annu. Rev. Cell Biol. v.7 Mechanisms and function of cell death Ellis, R.E.;Yuan, J.Y.;Horvitz, H.R. https://doi.org/10.1146/annurev.cb.07.110191.003311
  3. Science v.267 Apoptosis in the pathogenesis and treatment of disease Thompson, C.B. https://doi.org/10.1126/science.7878464
  4. Science v.281 Caspases: Enemies within Thornberry, N.A.;Lazebnik, Y. https://doi.org/10.1126/science.281.5381.1312
  5. Annu. Rev. Biochem. v.68 Mammalian caspases: Structure, activation, substrates, and functions during apoptosis Earnshaw, W.C.;Martins, L.M.;Kaufmann, S.H. https://doi.org/10.1146/annurev.biochem.68.1.383
  6. Leuk. Lymphoma v.19 Apoptosis overview emphasizing the role of oxidative stress, DNA damage and signal-transduction pathways Payne, C.M.;Bernstein, C.;Bernstein, H. https://doi.org/10.3109/10428199509059662
  7. Annu. Rev. Biochem. v.69 Apoptosis signaling Strasser, A.;O'Connor, L.;Dixit, V.M. https://doi.org/10.1146/annurev.biochem.69.1.217
  8. Pancreas v.22 Caspase-3 activation downstream from reactive oxygen species in heat-induced apoptosis of pancreatic carcinoma cells carrying a mutant p53 gene Kobayashi, D.;Sasaki, M.;Watanabe, N. https://doi.org/10.1097/00006676-200104000-00005
  9. Histopathology v.33 Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma Virkajarvi, N.;Paakko, P.;Soini, Y. https://doi.org/10.1046/j.1365-2559.1998.00553.x
  10. J. Biol. Chem. v.275 Inhibition of caspase-3-mediated poly (ADP-ribose) polymerase (PARP) apoptotic cleavage by human PARP autoantibodies and effect on cells undergoing apoptosis Decker, P.;Isenberg, D.;Muller, S. https://doi.org/10.1074/jbc.275.12.9043
  11. Science v.278 Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis Kothakota, S.;Azuma, T.;Reinhard, C.;Klippel, A.;Tang, J.;Chu, K. https://doi.org/10.1126/science.278.5336.294
  12. Arch. Pham. Res. v.21 Structural analogues of cumambrin B from the flower of Chrysanthemum boreale Jang, D.S.;Yang, M.S.;Ha, T.J.;Park, K.H. https://doi.org/10.1007/BF02975380
  13. Planta Med. v.69 Antibacterial activity and chemical composition of essential oil of Crysanthemum boreale Kim, K.J.;Kim, Y.H.;Yu, Y.H.;Jeong, S.I.;Cha, J.D.;Kil, B.S.;You, Y.O. https://doi.org/10.1055/s-2003-38479
  14. J. Tradit. Chin. Med. v.3 Preliminary observation of 131Cs distribution in experimental acute myocardial infection and coronary insufficiency treated with root of Salvia miltiorrhiza, flower of Chrysanthemum morifolium and Chrysanthemum indicum Chen, K.Y.;Wen, S.F.;Zhi, Z.J.;Shao, J.S.
  15. Peiching Hsueh Yuan Hsueh Pao v.14 Separation and identification of flavonoids from the flower of Chrysanthemum indicum He, Y.Q.;Li, R.Z.;S'hen, L.
  16. Bioorg. Med. Chem. v.11 Cytotoxic versus anti-inflammatory effects in HeLa, Jurkat T and human peripheral blood cells caused by guaianolide-type sesquiterpene lactones Hilmi, F.;Gertsch, J.;Bremner, P.;Valovic, S.;Heinrich, M.;Sticher, O.;Heilmann, J. https://doi.org/10.1016/S0968-0896(03)00346-8
  17. Planta Med. v.67 A new guaianolide as apoptosis inhibitor from Chrysanthemum boreale Lee, J.R.;Yang, M.S.;Jang, D.S.;Ha, T.J.;Park, K.M.;Lee, C.H. https://doi.org/10.1055/s-2001-16477
  18. Sichuan Da Xue Xue Bao Yi Xue Ban v.35 Induction of apoptosis of cultured hepatocarcinoma cell by essential oil of Artemisia annul L Li, Y.;Li, M.Y.;Wang, L.;Jiang, Z.H.;Li, W.Y.;Li, H.
  19. In Vivo v.17 Induction of apoptosis by Citrus paradisi essential oil in human leukemic (HL-60) cells Hata, T.;Sakaguchi, I.;Mori, M.;Ikeda, N.;Kato, Y.;Minamino, M.;Watabe, K.
  20. Anticancer Res. v.19 The induction of apoptosis in human melanoma, breast and ovarian cancer cell lines using an essential oil extract from the conifer Tetraclinis articulata Buhagiar, J.A.;Podesta, M.T.;Wilson, A.P.;Micallef, M.J.;Ali, S.
  21. Food Sci. Biotechnol. v.11 Glycyrrhetinic acid inhibits cell growth and proliferation, and induces apoptosis in human gingival fibroblasts Kim, K.J.;Yoo, S.K.;Kim, I.S.;Kim, S.M.;Lee, S.J.;Yu, H.H.;You, Y.O.
  22. Biochem. Pharmacol. v.63 Wogonin and fisetin induce apoptosis in human promyeloleukemic cells, accompanied by a decrease of reactive oxygen species, and activation of caspase-3 and $Ca^{2+}$-dependent endonuclease Lee, W.R.;Shen, S.C.;Lin, H.Y.;Hou, W.C. https://doi.org/10.1016/S0006-2952(01)00876-0
  23. J. Gene Med. The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adenoassociated virus (rAAV) transgene expression Li, L.;Yang, L.;Kotin, R.M.
  24. Food Sci. Biotechnol. v.13 Resveratrol induces apoptosis in SW480 human colon cancer cell lines Lee, H.S.;Sur, E.Y.;Kim, W.K.
  25. Oncol. Rep. v.9 Specific induction of apoptosis by 1,8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line Moteki, H.;Hibasami, H.;Yamada, Y.;Katsuzaki, H.;Imai, K.;Komiya, T.
  26. Exp. Cell Res v.269 Poly (ADP-ribose) polymerase cleavage during apoptosis: when and where? Soldani, C.;Iaze, M.C.;Bottone, M.G.;Tognon, G.;Boggiogera, M.;Pellicciari, C.E.;Scovassi, A.I. https://doi.org/10.1006/excr.2001.5293
  27. Free Radic. Biol. Med. v.31 $Ca^{2+}$_calmodulin antagonist chlorpromazine and poly (ADP-ribose) polymerase modulators 4-aminobenzamide and nicotinamide influence hepatic expression of BCL-XL and P53 and protect against acetaminophen-induced programmed and unprogrammed cell death in mice Ray, S.D.;Balasubramanian, G.;Bagchi, D.;Reddy, C.S. https://doi.org/10.1016/S0891-5849(01)00562-7