Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of Vibrio parahaemolyticus OMPs and Production of Antibodies against OMPs

  • Kim, Soo-Min (Interdisciplinary Program in Bioinformatics, Seoul National University) ;
  • Noh, Bong-Soo (Dept. of Food and Microbial Technology, Seoul Women's University) ;
  • Kim, Hae-Yeong (Dept. of Food Biotechnology, Institute of Life Science, Kyung Hee University) ;
  • Park, Se-Jin (Dept. of Food Science and Nutrition & Research Institute of Human Ecology, Seoul National University) ;
  • Ji, Geun-Eog (Dept. of Food Science and Nutrition & Research Institute of Human Ecology, Seoul National University)
  • Published : 2005.06.30
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Abstract

Vibrio parahaemolyticus is a gram-negative bacterium which acts as a causative agent for food poisoning. Studies with respect to specific extracellular proteins of V. parahaemolyticus would be useful for the development of specific detection methods against V. parahaemolyticus. In our present study, outer membrane proteins (OMPs) of V. parahaemolyticus were obtained from insoluble traction of 1% sarkosyl treated-cell wall materials. SDS-PAGE analysis showed the presence of several conserved outer membrane proteins among five strains of V. parahaemolyticus, and three bands were identified as V. parahaemolyticus OMPs through MALDI-TOF analysis. Polyclonal antibodies enriched with anti-OmpU were obtained from immunized rabbits. The antibodies against these proteins may be useful for the development of detection methods for V. parahaemolyticus.

Keywords

References

  1. J. Bacteriol. v.184 Identification and characterization of pvuA, a gene encoding the ferric vibrioferrin receptor protein in Vibrio parahaemolyticus Funahashi, T.;Moriya, K.;Uemura, S.;Miyoshi, S.;Shinoda, S.;Narimatsu, S.;Yamamoto, S. https://doi.org/10.1128/jb.184.4.936-946.2002
  2. Cellular microbiology: bacteria-host interactions in health and disease Hendeson, B.;Wilson, M.;McNab, R.;Lax, A.J.
  3. J. Bacteriol. v.183 Vibrio fischeri outer membrane protein OmpU plays a role in normal symbiotic colonization Aeckersberg, F.;Lupp, C.;Feliciano, B.;Ruby, E.G. https://doi.org/10.1128/JB.183.22.6590-6597.2001
  4. Int. J. Food Microbiol. v.29 Rapid detection of Vibrio parahaemolyticus in oysters by immunofluorescence microscopy Chen, H.C.;Chang, T.C. https://doi.org/10.1016/0168-1605(95)00049-6
  5. Infect. Immun. v.68 Identification of group B streptococcal Sip protein, which elicits cross-protective immunity Brodeur, B.R.;Boyer, M.;Charlebois, I.;Hamel, J.;Couture, F.;Rioux, C.R.;Martin, D. https://doi.org/10.1128/IAI.68.10.5610-5618.2000
  6. Microbiol. Immunol. v.30 Composition of major outer membrane proteins of Vibrio vulnificus isolates: effect of different growth media and iron deficiency Koga, T.;Kawata, T.
  7. J. Bacteriol. v.115 Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate Filip, C.;Fletcher, G.;Wulff, J.L.;Earhart, C.F.
  8. Infect. Immun. v.68 impA, a gene coding for an inner membrane protein, influences colonial morphology of Actinobacillus actinomycetemcomitans Mintz, K.P.;Fives-Taylor, P.M. https://doi.org/10.1128/IAI.68.12.6580-6586.2000
  9. Molecular cloning: A Laboratory Manual Sambrook, J.;Fritch, E.F.;Maniatis, T.
  10. Nature v.379 Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry Wilm, M.;Shevchenko, A.;Houthaeve, T.;Breit, S.;Schweigerer, L.;Fotsis, T.;Mann, M. https://doi.org/10.1038/379466a0
  11. J. Chromatogr. A v.826 Examination of micro-tip reversed-phase liquid chromatographic extraction of peptide pools for mass spectrometric analysis Erdjument-Bormage, H.;Lui, M.;Lacomis, L.;Grewal, A.;Annan, R.S.;McNultry, D.E.;Carr, S.A.;Tempst, P. https://doi.org/10.1016/S0021-9673(98)00705-5
  12. J. Am. Soc. Mass Spectrom. v.14 Identification of 2D-gel proteins: A comparison of MALD/TOF peptide mass mapping to {$\mu$} LC-ESI tandem mass spectrometry Lim, H.;Eng, J.;Yates, J.R. III.;Tollaksen, S.L.;Giometti, C.S.;Holden, J.F.;Adams, M.W.;Reich, C.I.;Olsen, G.J.;Hays, L.G. https://doi.org/10.1016/S1044-0305(03)00144-2
  13. Proteomics v.1 Strategy for protein isoform identification from expressed sequence tags and its application to peptide mass fingerprinting Lisacek, F.C.;Traini, M.D.;Sexton, D.;Harry, J.L.;Wilkins, M.R.
  14. Proc. Natl. Acad. Sci. USA v.76 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Towbin, H.;Staehelin, T.;Gordon, J.
  15. J. Struct. Biol. v.121 General and specific porins from bacterial outer membranes Schirmer, T. https://doi.org/10.1006/jsbi.1997.3946
  16. J. Bacteriol. v.178 Porins of Vibrio cholerae: purification and characterization of OmpU Chakrabarti, S.R.;Chaudhuri, K.;Sen, K.;Das, J.