Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Identification of Hanwoo and Holstein meat using MGB probe based real-time PCR associated with single nucleotide polymorphism (SNP) in Melanocortin 1 receptor (MC1R) gene

소 모색관련 MC1R 유전자의 SNP와 관련한 MGB probe에 기초한 real-time PCR을 이용한 한우육과 Holstein육의 판별

  • Park, Sung-Do (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Tae-Jung (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jae-Il (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2005.02.05
  • Published : 2005.03.25
Download PDF
⟨ Previous Next ⟩

Abstract

The melanocortin 1 receptor (MC1R) plays an important role in regulation of melanin pigment synthesis within mammalian melanocytes. Mutations within the gene encoding MC1R have been shown to explain coat color variations within several mammalian species including cattle. To develope a rapid and accurate method for the identification of Hanwoo meat, we performed a single nucleotide polymorphism (SNP) analysis in Melanocortin 1 receptor (MC1R) gene using TaqMan$^{(R)}$ MGB probe-based real-time PCR. Two specific probes (one for Hanwoo and the other for Holstein and Black angus) were designed. At the 5' end of 2 TaqMan$^{(R)}$ MGB probes, 6-carboxyfluorescein (FAM) was labeled for Hanwoo, and VIC for Holstein and Black angus. As a result, Hanwoo samples showed FAM-positive signal only, whereas other samples showed VIC-positive. This result suggests that the TaqMan$^{(R)}$ MGB probe based real-time PCR technique would be very accurate, easy and reproducible method to discriminate between Hanwoo meat and Holstein/Black angus meat.

Keywords

References

  1. 김태현, 윤두학, 박웅우, 이혜영, 오정종, 정일정, 탁태영, 김정남, 한재용, 소 품종별 Melanocortin Receptor 1(MC1R) 유전자의 유전자형 빈도에 관한 연구. 한국동물자원과학회지 2000, 42, 735-744
  2. 민병록, 한재용, 이무하, RAPD 기법을 이용한 쇠고기의 품종(한우육, 유우육 (Holstein육), 수입우육) 구분. 한국축산학회지 1995, 37, 651-660
  3. 이성수, 양영훈, 강승률, 오운용, 양보석, 고서봉, 오성종, 김규일. 한우, 제주재래흑우, 흑모회우와 갈모회우에서의 MSH Receptor(MC1R) 유전자의 유전자형 및 빈도 비교. 한국동물자원과학회지 2000, 42, 253-260
  4. 정의룡, 김우태, 김연수, 한상기. 소 모색관련 유전자 MC1R의 PCR-RFLP Marker를 이용한 한우육 판별. 한국동물자원과학회지 2000, 42, 379-390
  5. 정의룡, 김우태, 김연수, 한상기. PCR-SSCP 기법을 이용한 소 MC1R 유전자의 다형성 분석 및 한우육 감별. 한국동물자원과학회지 2001, 43. 45-52
  6. 홍영호, 정일정, 김태헌, 김회발, 윤두학, 김형선, 조병욱, 한채용. 품종 특이성을 이용한 한우 판별 표지인자 개발. 한국동물유전육종학회지 1998, 2, 107-114
  7. Cone RD, Lu D, Koppula S, Vage DI, Klungland K, Boston B, Chen W, Orth DN, Pouton C, Kesterson RA. The Melanocortin Receptors : Agonists, Antagonists, and the Hormonal Control of Pigmentation. Recent Prog Horm Res 1996, 51, 287-317
  8. de Kok JB, Wiegerinck ET, Giesendorf BA, Swinkels DW. Rapid Genotyping of Single Nucleotide Polymorphisms Using Novel Minor Groove Binding DNA Oligonucleotides (MGB Probes). Hum Mutat 2002, 19, 554-559 https://doi.org/10.1002/humu.10076
  9. Joerg H, Fries HR, Meijerink E, Stranzinger GF. Red coat color in Holstein cattle is associated with a deletion in the MSHR gene. Mamm Genome 1996, 7, 317-318 https://doi.org/10.1007/s003359900090
  10. Livak KJ. Allelic discrimination using fluorogenic probes and the 5' nuclease assay. Genet Anal 1999, 14, 143-149 https://doi.org/10.1016/S1050-3862(98)00019-9
  11. McCarthy JJ, Hilfiker R. The use of single-nucleotide polymorphism maps in pharmacogenomics. Nat Biotechnol 2000, 18, 505-508 https://doi.org/10.1038/75360
  12. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988, 16, 1215 https://doi.org/10.1093/nar/16.3.1215
  13. Nebert DW. Pharmacogenetics and pharmacogenomics : Why is this relevant to the clinical geneticist? Clin Genet 1999, 56, 247-258 https://doi.org/10.1034/j.1399-0004.1999.560401.x
  14. Smith EJ, Jones CP, Bartlett J, Nestor KE. Use of randomly amplified polymorphic DNA markers for the genetic analysis of relatedness and diversity in chickens and turkeys. Poultry Sci 1996, 75, 579-584 https://doi.org/10.3382/ps.0750579
  15. Vage DI, Klungland H, Lu D, Cone RD. Molecular and pharmacological characterization of dominant black coat color in sheep. Mamm Genome 1999, 10, 39-43 https://doi.org/10.1007/s003359900939
  16. Wang D, Fan J, Siao D, Berno A, Young P, Sapolsky R, Ghandour G, Perkins N, Winchester E, Spencer J, Kruglyak L, Stein L, Jsie L, Topaloglou T, Hubbell E, Robinson E, Mittmann M, Morris M, Shen N, Kilburn D, Rioux J, Nusbaum C, Rozen S, Hudson T, Lipshutz R, Chee M, Lander E. Largescale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science 1998, 280, 1077-1082 https://doi.org/10.1126/science.280.5366.1077