Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Expression and diagnostic application of p12 protein of African swine fever virus by recombinant baculovirus

재조합 baculovirus에 의한 아프리카 돼지콜레라바이러스 p12 단백질의 발현과 진단적 적용

  • Choi, Kang-Seuk (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service) ;
  • Choi, Cheong-up (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service) ;
  • Kim, Yong-Joo (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service)
  • Accepted : 2005.02.21
  • Published : 2005.03.25
Download PDF
⟨ Previous Next ⟩

Abstract

African swine fever (ASF) is an infectious disease of domestic and wild pigs for which there is no vaccine in the world. A proper surveillance of viral activity and a timely response to ASF outbreaks depend upon the rapid diagnosis of ASF viral infection. Internationally prescribed enzyme-linked immunosorbent assay (ELISA) is a fast, sensitive test routinely used in the diagnosis of the ASF. However, inactivated whole ASF virus antigen used in this test is a tedious to prepare and has a risk of outside exposure of infectious virus by laboratory accident during the preparation. An ASF virus noninfectious recombinant antigen is a safe and easily produced alternative antigen for use in diagnostic assay. We have cloned the ORF O61R gene of the ASF virus to generate a recombinant baculovirus producing the p12 protein in insect cells under control of the polyhedrin promoter as non-fusion protein. When used in an indirect ELISA, the p12 antigen showed reactivity with all known ASF positive pig sera but not with negative pig sera. Our results indicated that the p12 protein would be one of alternative antigens for diagnosis of the ASF.

Keywords

References

  1. Alcami A, Angulo A, Lopez-Otin C, Munoz M, Freije JM, Carrascosa AL, Vinuela E. Amino acid sequence and structural properties of protein p12, an African swine fever virus attachment protein. J Virol 1992, 66, 3860-3868
  2. Alcami A, Carrascosa AL, Vinuela E. The entry of African swine fever virus into Vero cells. Virology 1989, 171, 68-75 https://doi.org/10.1016/0042-6822(89)90511-4
  3. Alcaraz C, De Diego M, Pastor MJ, Escribano JM. Comparisn of a radioimmunoprecipitation assay to immunoblotting and ELISA for detection of antibody to African swine fever virus. J Vet Diagn Invest 1990, 2, 191-196 https://doi.org/10.1177/104063879000200307
  4. Alcaraz C, Rodriguez F, Oviedo JM, Eiras A, De Diego M, Alonso C, Escribano JM. Highly specific confirmatory western blot test for African swine fever virus antibody detection using the recombinant virus protein p54. J Virol Methods 1995, 52, 111-119 https://doi.org/10.1016/0166-0934(94)00150-F
  5. Angulo A, Vinuela E, Alcami A. Comparison of the sequence of the gene encoding African swine fever virus attachment protein p12 from field virus isolates and viruses passaged in tissue culture. J Virol 1992, 66, 3869-3872
  6. Angulo A, Vinuela E, Alcami A. Inhibition of African swine fever virus binding and infectivity by purified recombinant virus attachment protein p12. J Virol 1993, 67, 5463-5471
  7. Arias M, Sanchez Vizcaino JM. Manual de diagnostico serologico de la peste porcina africana (Manual of diagnostic serology for African Swine fever.). pp.1-44, Ministry of Agriculture, CISA-INIA, Valdeolmos-28130, Madrid, 1992
  8. Barderas MG, Wigdorovitz A, Merelo F, Beitia F, Alonso C, Borca MV, Escribano JM. Serodiagnosis of African swine fever using the recombinant protein p30 expressed in insect larvae. J Virol Methods 2000, 89, 129-136 https://doi.org/10.1016/S0166-0934(00)00209-3
  9. Bech-Nielsen S, Fernandez J, Martinez-Pereda F, Espinosa J, Perez Bonilla Q, Sanchez-Vizcaino JM. A case study of an outbreak of African swine fever in Spain. Br Vet J 1995, 151, 203-214 https://doi.org/10.1016/S0007-1935(95)80012-3
  10. Carrascosa AL, del Val M, Santaren JF, Vinuela E. Purification and properties of African swine fever virus. J Virol 1985, 54, 337-344
  11. Carrascosa AL, Sastre I, Vinuela E. African swine fever virus attachment protein. J Virol 1991, 65, 2283-2289
  12. Coggins L. African swine fever virus. Pathogenesis. Prog Med Virol 1974, 18, 48-63
  13. Dixon LK, Costa JV, Escribano JM, Rock DL, Vinuela E, Wilkinson PJ. The Asfarviridae. In: Van Regenmortel MHV, Fauquet CM, Bishop DHL, Carsten EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (ed.), Virus Taxonomy, 7th Report of the International Committee for the Taxonomy of Viruses, pp. 159-165, Academic Press, New York, 2000
  14. Forman AJ, Wardley RC, Wilkinson PJ. The immunological response of pigs and guinea pigs to antigens of African swine fever virus. Arch Virol 1982, 74, 91100
  15. Kihm U, Ackerman M, Mueller H, Pool R. Approaches to vaccination. In: Becker Y(Ed.) African Swine Fever, pp. 127-144, Martinus Nijhoff Publishing, Boston, 1987
  16. Mebus CA. African swine fever. Adv Virus Res 1988, 35, 251-269 https://doi.org/10.1016/S0065-3527(08)60714-9
  17. Leitao A, Cartaxeiro C, Coelho R, Cruz B, Parkhouse RME, Portugal FC, Vigario JD, Martins CLV. The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response. J Gen Virol 2001, 82, 513-523 https://doi.org/10.1099/0022-1317-82-3-513
  18. Oviedo JM, Rodriguez F, Gomez-Puertas P, Brun A, Gomez N, Alonso C, Escribano JM. High level expression of the major antigenic African swine fever virus proteins p54 and p30 in baculovirus and their potential use as diagnostic reagents. J Virol Methods 1997, 64, 27-35 https://doi.org/10.1016/S0166-0934(96)02140-4
  19. Pastor MJ, Arias M, Escribano JM. Comparison of two antigens for use in an enzyme-linked immunosorbent assay to detect African swine fever antibody. Am J Vet Res 1990, 51, 1540-1543
  20. Penrith ML, Thomson GR, Bastos ADS. African swine fever. In: Coetzer JAW, Tustin RC, Eds, Infectious Diseases of Livestock with Special Reference to Southern Africa, 2nd ed, pp.1087-1119, Oxford University Press, Cape Town, 2004
  21. Tabares E, Martinez J, Martin E, Escribano JM. Proteins specified by African swine fever virus: IV. Glycoproteins and phosphoproteins. Arch Virol 1983, 77, 167-180
  22. Vidal MI, Stiene M, Henkel J, Bilitewski U, Costa JV, Oliva AG. A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of African swine fever virus antigens and antibodies. J Virol Methods 1997, 66, 211-218 https://doi.org/10.1016/S0166-0934(97)00059-1
  23. Vinuela E. African swine fever. Curr Top Microbiol Immunol 1985, 116, 151-170
  24. Yanez RJ, Rodriguez JM, Nogal ML, Yuste L, Enriquez C, Rodriguez JF, Vinuela E. Analysis of the complete nucleotide sequence of African swine fever virus. Virology 1995, 208, 249-278 https://doi.org/10.1006/viro.1995.1149