Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Effect of Melatonin on Biochemical Changes after Ischemia-Reperfusion Injury of Rat Skeletal Muscle

흰쥐 골격근의 허혈-재관류 손상후 생화학적 변화에 미치는 Melatonin의 효과

  • Park, Hye June (Department of Plastic and Reconstructive Surgery, Ilsan Paik Hospital, Inje University College of Medicine) ;
  • Burm, Jin Sik (Department of Plastic and Reconstructive Surgery, Ewha Womans University College of Medicine)
  • 박혜준 (인제대학교 의과대학 일산백벽원 성형외과학교실) ;
  • 범진식 (이화여자대학교 의과대학 성형외과학교실)
  • Received : 2005.07.28
  • Published : 2005.11.10
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Abstract

The ischemia-reperfusion injury of the skeletal muscles is caused by generation of reactive oxygen during ischemia and reperfusion. Melatonin or N-Acetyl-5-methoxy- tryptamine is suggested to have antioxidant effects in several tissues. In present study, we examined the protective effect of melatonin in a rat hind limb ischemia-reperfusion injury. Dimethyl-sulfoxide(DMSO) was also tested for comparison. Ischemia was induced for 4 hours by vascular clamping and followed by 1 hour or 24 hours of reperfusion. Muscle injury was evaluated in 4 groups such as single laparotomy group(control), ischemia-reperfusion group, DMSO group, melatonin group. Eedema ratio and malondialdehyde(MDA) of muscle tissue and serum level of creatine kinase(CK), were measeured at the end of reperfusion. DMSO and melatonin group showed significant amelioration of edema and serum CK compared with ischemia-reperfusion group. The decreasing effect was more prominent in melatonin group. The muscle tissue MDA concentration is significantly lower in melatonin group than in ischemia-reperfusion group. The results show that melatonin prevents and improves ischemia-reperfusion injury more effectively in a rat hind limb than DMSO dose. Thus, clinically the melatonin may be used for a beneficial treatment of such injuries

Keywords

References

  1. McCord JM: Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 312: 159, 1985 https://doi.org/10.1056/NEJM198501173120305
  2. Tan DX, Poeggeler B, Reiter RJ, Chen LD, Chen S, Manchester LC, Barlow-Walden LR: The pineal hormone melatonin inhibits DNA-adduct formation induced by the chemical carcinogen safrole in vivo. Cancer Lett 15: 65, 1993
  3. Tan DX, Manchester LC, Reiter RJ, Qi W, Kim SJ, El-Sokkary GH: Ischemia/ reperfusion induced arrhythmias in the isolated rat heart; prevention by melatonin. J Pineal Res 25: 184, 1998 https://doi.org/10.1111/j.1600-079X.1998.tb00558.x
  4. Chen YY, Kim MC, Ko YG, Baik HH, Cho YH: Effect of melatonin during recovery of tissue injury after intestine ischemia-reperfusion. Kor J Emer Medi 14: 264, 2003
  5. Bradford MM: A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248, 1976 https://doi.org/10.1016/0003-2697(76)90527-3
  6. Bozkurt AK: $\alpha$-tocopherol(vitamin E) and iloprost attenuate reperfusion injury in skeletal muscle ischemia/ reperfusion injury. J Cardiovasc Surg 43: 693, 2002
  7. Feller AM, Roth AC, Russell RC, Eagleton B, Suchy H, Debs N: Experimental evaluation of oxygen free radical scavengers in the prevention of reperfusion injury to skeletal muscle. Ann Plast Surg 22: 321, 1989 https://doi.org/10.1097/00000637-198904000-00007
  8. Reiter RJ, Carneiro RC, Oh CS: Melatonin in relation to cellular antioxidative defense mechanisms. Horm Metab Res 29: 363, 1997 https://doi.org/10.1055/s-2007-979057
  9. Joo JY, Uz T, Manev H: Opposite effects of pinealectomy and melatonin administration on brain damage following cerebral focal ischemia in rat. Restor Neurol Neurosci 13: 185, 1998
  10. Cheung RTF: The utility of melatonin in reducing cerebral damage resulting from ischemia and reperfusion. J Pineal Res 34: 153, 2003 https://doi.org/10.1034/j.1600-079X.2003.00034.x
  11. Sener G, Sehirli AO, Paskaloglu K, Dulger GA, Alican I: Melatonin treatment protects against ischemia/reperfusion-induced functional and biochemical changes in rat urinary bladder. J Pineal Res 34: 226, 2003 https://doi.org/10.1034/j.1600-079X.2003.00038.x
  12. Sayan H, Ozacmak VH, Ozen OA, Coskun O, Arslan SO, Sezen SC, Aktas RG: Beneficial effects of melatonin on reperfusion injury in rat sciatic nerve. J Pineal Res 37: 143, 2004 https://doi.org/10.1111/j.1600-079X.2004.00145.x
  13. Ressmeyer AR, Mayo JC, Zelosko V, Sainz RM, Tan DX, Poeggeler B, Antolin I: Antioxidant properties of $N^{1}$-acetyl-5-methoxy-kynuramine(AMK); scavenging of free radicals and prevention of protein destruction. Redox Rept 8: 205, 2003 https://doi.org/10.1179/135100003225002709
  14. Nordlund JJ, Lerner AB: The effects of oral melatonin on skin color and on the release of pituitary hormones. J Clin Endocrinol Metab 45: 768, 1977 https://doi.org/10.1210/jcem-45-4-768