Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Highly Polymorphic Bovine Leptin Gene

  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Cho, B.H. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Park, B.L. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Choi, Y.H. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Cheong, H.S. (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Lee, H.K. (Department of Biotechnology, Hankyong National University) ;
  • Chung, E.R. (Department of Biotechnology, Sangi University) ;
  • Cheong, I.C. (National Livestock Research Institute, RDA) ;
  • Shin, H.D. (Department of Genetic Epidemiology, SNP Genetics, Inc.)
  • Received : 2004.12.21
  • Accepted : 2005.05.31
  • Published : 2005.11.01
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Abstract

The leptin, an anti-obesity protein, is a hormone protein expressed and secreted mainly from adipocyte tissue, and involved in regulation of body weight, food intake and energy metabolism. In an effort to discover polymorphism(s) in genes whose variant(s) might be implicated in phenotypic traits of growth, we have sequenced exons and their boundaries of leptin gene including 1,000 bp upstream of promoter region with twenty-four unrelated Korean cattle. Fifty-seven sequence variants were identified: fourteen in 5' flanking region, twenty-seven in introns, eight in exons, and eight in 3' flanking region. By pair-wise linkage analysis among polymorphisms, ten sets of SNPs were in absolute linkage disequilibrium (LD) (|D'| = 1 and $r^2$ = 1). Among variants identified, thirty-six SNPs were newly identified, and twenty-one SNPs, which were reported in other breeds, were also confirmed in Korean cattle. The allele frequencies of variants were quite different among breeds. The information from SNPs of bovine leptin gene could be useful for further genetic studies of this gene.

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References

  1. Ahima, R. S., C. B. Saper, J. S. Flier and J. K. Elmquist. 2000. Leptin regulation of neuroendocrine systems. Frontiers in Neuroendocrinology 21:263-307.
  2. Buchanan, F. C., C. J. Fitzsimmons, A. G. Van Kessel, T. D. Thue, D. C. Winkelman-Sim and S. M. Schmutz. 2002. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genetics Selection Evolution 34:105-116.
  3. Di Marzo, V., S. K. Goparaju, L. Wang, J. Liu, S. Batkai, Z. Jarai, F. Fezza, G. I. Miura, R. D. Palmiter, T. Sugiura and G. Kunos. 2001. Leptin-regulated endocannabinoids are involved in maintaining food intake. Nature 410:822-825.
  4. Fitzsimmons, C. J., S. M. Schmutz, R. D. Bergen and J. J. McKinnon. 1998. A potential association between the BM 1500 microsatellite and fat deposition in beef cattle. Mammalian Genome 9:432-434.
  5. Halaas, J. L., K. S. Gajiwala, M. Maffei, S. L. Cohen, B. T. Chait, D. Rabinowitz, R. L. Lallone, S. K. Burley and J. M. Friedman. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Sci. 269:543-546.
  6. Hedrick, P. and S. Kumar. 2001. Mutation and linkage disequilibrium in human mtDNA. European Journal of Human Genetics 9:969-972.
  7. Hedrick, P. W. 1987. Gametic disequilibrium measures: proceed with caution. Genetics 117:331-341.
  8. Jeon, G. J., H. Y. Chung, J. G. Choi, M. S. Lee, C. W. Lee, J. J. Park, J. M. Ha, H. K. Lee and H. H. Sung. 2005. Relationship between Genetic Variants of Mitochondrial DNA and Growth Traits in Hanwoo Cattle. Asian-Aust. J. Anim. Sci. 18:301-307.
  9. Konfortov, B. A., V. E. Licence and J. R. Miller. 1999. Resequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon. Mammalian Genome 10:1142-1145. https://doi.org/10.1007/s003359901180
  10. Lagonigro, R., P. Wiener, F. Pilla, J. A. Woolliams and J. L. Williams. 2003. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Anim. Genet. 34:371-374. https://doi.org/10.1046/j.1365-2052.2003.01028.x
  11. Lee, G. H., R. Proenca, J. M. Montez, K. M. Carroll, J. G. Darvishzadeh, J. I. Lee and J. M. Friedman. 1996. Abnormal splicing of the leptin receptor in diabetic mice. Nature 379:632-635.
  12. Pelleymounter, M. A., M. J. Cullen, M. B. Baker, R. Hecht, D. Winters, T. Boone and F. Collins. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Sci. 269:540-543.
  13. Schwartz, M. W., R. J. Seeley, L. A. Campfield, P. Burn and D. G. Baskin. 1996. Identification of targets of leptin action in rat hypothalamus. J. Clin. Invest. 98:1101-1106. https://doi.org/10.1172/JCI118891
  14. Stephens, M., N. J. Smith and P. Donnelly. 2001. A new statistical method for haplotype reconstruction from population data. Am. J. Hum Genet. 68:978-989.
  15. Stone, R. T., S. M. Kappes and C. W. Beattie. 1996. The bovine homolog of the obese gene maps to chromosome 4. Mammalian Genome 7:399-400. https://doi.org/10.1007/s003359900119
  16. Wheeler, T. L., L. V. Cundiff and R. M. Koch. 1994. Effect of marbling degree on beef palatability in Bos taurus and Bos indicus cattle. J. Anim. Sci. 72:3145-3151.
  17. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold and J. M. Friedman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-432.

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