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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Molecular Cloning, Phylogenetic Analysis, Expressional Profiling and In Vitro Studies of TINY2 from Arabidopsis thaliana

  • Wei, Gang (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Pan, Yi (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Lei, Juan (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Zhu, Yu-Xian (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, National Plant Gene Research Center)
  • Published : 2005.07.31
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Abstract

A cDNA that was rapidly induced upon abscisic acid, cold, drought, mechanical wounding and to a lesser extent, by high salinity treatment, was isolated from Arabidopsis seedlings. It was classified as DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and was closely related to the TINY gene, we named it TINY2. Gel retardation assay revealed that TINY2 was able to form a specific complex with the previously characterized DRE element while showed only residual affinity to the GCC box. When fused to the GAL4 DNA-binding domain, either full-length or its C-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay while its N-terminus was completely inactive. Our data indicate that TINY2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream genes in response to environmental stress.

Keywords

References

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