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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Studies on the Epitope of Neuronal Growth Inhibitory Factor (GIF) with Using of the Specific Antibody

  • Pang, Li-Yan (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University) ;
  • Ru, Bing-Gen (The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University)
  • Published : 2005.11.30
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Abstract

Human neuronal growth inhibitory factor (GIF), a metalloprotein classified as metallothionein-3, is specifically expressed in mammal central nervous system (CNS). In these Studies the specific antibody to human GIF was prepared and used to search the epitope of human GIF by enzyme-linked immunosorbent assay (ELISA) and sequence comparison. The result of ELISA showed the epitope of human GIF may locate on a octapeptide (EAAEAEAE) in the $\alpha$-domain of human GIF, and the result of nerve cell culture indicated that the biological activity of GIF may be affected by the specific antibody.

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References

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