Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of Additives on the Fermentation Quality and Residual Mono- and Disaccharides Compositions of Forage Oats (Avena sativa L.) and Italian Ryegrass (Lolium multiflorum Lam.) Silages

  • Shao, Tao (Department of Grassland and Forage Science, College of Animal Sciences and Technology Nanjing Agricultural University) ;
  • Shimojo, M. (Laboratory of Animal Feed Science, Division of Animal Science, Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University) ;
  • Wang, T. (Department of Grassland and Forage Science, College of Animal Sciences and Technology Nanjing Agricultural University) ;
  • Masuda, Y. (Laboratory of Animal Feed Science, Division of Animal Science, Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University)
  • Received : 2004.09.26
  • Accepted : 2005.04.22
  • Published : 2005.11.01
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Abstract

This study aimed to evaluate the effects of silage additives on the fermentation qualities and residual mono- and disaccharides composition of silages. Forage Oats (Avena sativa L.) and Italian Ryegrass (Lolium multiflorum Lam.) were ensiled with glucose, sorbic acid and pre-fermented juice of epiphytic lactic acid bacteria (FJLB) treatments for 30 days. In both species grass silages, although the respective controls had higher contents of butyric acid (20.86, 33.45g $kg^{-1}$ DM) and ammonia-N/total nitrogen (100.07, 114.91 g $kg^{-1}$) as compared with other treated silages in forage oats and Italian ryegrass, the fermentation was clearly dominated by lactic acid bacteria. This was well indicated by the low pH value (4.27, 4.38), and high lactic acid/acetic acid (6.53, 5.58) and lactic acid content (61.67, 46.85 g $kg^{-1}$ DM). Glucose addition increased significantly (p<0.05) lactic acid/acetic acid, and significantly (p<0.05) decreased the values of pH and ammonia-N/total nitrogen, and the contents of butyric acid and volatile fatty acids as compared with control, however, there was a slightly but significantly (p<0.05) higher butyric acid and lower residual mono- and di-saccharides as compared with sorbic acid and FJLB additions. Sorbic acid addition showed the lowest ethanol, acetic acid and ammonia-N/total nitrogen, and highest contents of residual fructose, total mono- and di-saccharides and dry matter as well as high lactic acid/acetic acid and lactic acid content. FJLB addition had the lowest pH value and the highest lactic acid content, the most intensive lactic acid fermentation occurring in FJLB treated silages. This resulted in the faster accumulation of lactic acid and faster pH reduction. Sorbic acid and FJLB additions depressed clostridia or other undesirable bacterial fermentation, thus this decreased the water-soluble carbohydrates loss and saved the fermentable substrate for lactic acid fermentation.

Keywords

References

  1. Alli, I. and B. E. Baker. 1982. Effects of additives on lactic acid production and water soluble carbohydrates in chopped corn and alfalfa. J. Dairy Sci. 65:1472-1478.
  2. Alli, I., S. Pabari, R. Fairbairn and B. E. Baker. 1985. The effects of sorbates on the ensilage of chopped whole-plant maize and lucerne. J. Sci. Food Agric. 36:63-70.
  3. AOAC. 1984. Official Methods of Analysis. 14th edn. Association of Official and Analytical Chemists Arlington, Virginia-22201.
  4. Barker, S. B. and W. H. Summerson. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-554.
  5. Cai, Y., S. Kumai, J. G. Zhang and Y. Benno. 1999. Comparative studies of lactobacilli and enterococci associated with forage crops as silage inoculants. Anim. Sci. J. 70(4):188-194.
  6. Dewar, W. A. and P. McDonald. 1961. Determination of dry matter in silage by distillation with toluene. J. Sci. Food Agric. 12:790-795.
  7. Guan, W. T., F. Driehuis and P. V. Wikselaar. 2002. The influences of addition of sugar with or without L. buchneri on fermentation and aerobic stability of whole crop maize silage ensiled under anaerobic silos. Asian-Aust. J. Anim. Sci. 15(8): 1128-1133.
  8. Hattori, I., S. Kumai and R. Fukumi. 1996. Concentration of caproic and sorbic acids effective for inhibition of aerobic deterioration. Anim. Sci. Technol. 67:1101-1109.
  9. Henderson, A. R. and P. McDonald. 1971. Effect of formic acid on the fermentation of grass of low dry matter content. J. Sci. Food Agric. 22:157-163.
  10. Iwasaki, K., T. Nakui, M. Hayakawa and N. Tamada. 1978. Influence of the variety and harvesting date on the nutritive value of oat plant and the nutritive value of silages prepared from three different varieties of oats. J. Jap. Grassl. Sci. 23:348-355. In Japanese with English summary.
  11. Lacey, J., K. A. Lord and G. R. Cayley. 1981. Chemicals for preservative for moulding in damp hay. Anim. Feed Sci. Technol. 6:323-326.
  12. Masuko, T., S. Okada, T. Uchimura and K. Awaya. 1992. Effects of inoculation with lactic acid bacteria culture at ensiling on the fermentative quality and flora of lactic acid bacteria of grass silage. Anim Sci. Technol. 63:1182-1187. In Japanese with English summary.
  13. McDonald, P., A. R. Henderson and S. J. E. Heron. 1991. The biochemistry of silage. 2nd edn. Cambrian Printers Ltd. Aberystwyth. pp. 184-236.
  14. Ohshima, M., Y. Ohshima, E. Kimura and H. Yokota. 1997a. Fermentation quality of alfalfa and Italian ryegrass silages treated with previously fermented juices prepared from both the herbages. Anim. Sci. Technol. Jpn. 68:41-44.
  15. Ohshima, M., E. Kimura and H. Yokota. 1997b. A method of making good quality silage from direct cut alfalfa by spraying previously fermented juice. Anim. Feed Sci. Technol. 66:129-137.
  16. Ohshima, M., L. M. Cao, E. Kimura and H. Yokota. 1997c. Influence of addition of fermented green juice to alfalfa ensiled at different moisture contents. Grass. Sci. 43:56-58.
  17. Ohyama, Y., S. Masaki, A. Takigawa and T. Morichi. 1971. Studies on various factors affecting silage fermentation IX. Synergistic effect of inoculation of lactobacillus plantarum and addition of glucose on silage quality. Jap. J. Zootech. Sci. 42:1-8.
  18. Ohyama, Y., S. Masaki and T. Morichi. 1973. Effects of temperature and glucose addition on the process of silage fermentation. Jap. J. Zootech. Sci. 44:59-67.
  19. Ohyama, Y., T. Morichi and S. Masaki. 1975. The effect of inoculation with lactobacillus plantarum and the addition of glucose at ensiling on the quality of aerated silages. J. Sci. Food Agric. 26:1001-1008.
  20. Rooke, J. A. 1990. The numbers of epiphytic bacteria on grass at ensilage on commercial farms. J. Sci. Food Agric. 51:523-525.
  21. SAS Institute Inc. 1984. SAS/STAT User’s Guide:Version 6. 4th edn. SAS Institute Inc., Cary, North Carolina.
  22. Shao, T., N. Ohba, M. Shimojo and Y. Masuda. 2002. Dynamics of early fermentation of Italian ryegrass (Lolium multiflorum Lam.) silage. Asian-Aust. J. Anim. Sci. 15(11):1606-1610.
  23. Shao, T., N. Ohba, M. Shimojo and Y. Masuda. 2003. Effects of addition of glucose and sorbic acid on the fermentation quality of guineagrass (Panicum maximum Jacq.) silages. J. Fac. Agric. Kyushu. 47(2):351-358.
  24. Shao, T., N. Ohba, M. Shimojo and Y. Masuda. 2004. Effects of adding glucose, sorbic acid and pre-fermented juices on the fermentation quality of guineagrass (Panicum maximum Jacq.) silages. Asian-Aust. J. Anim. Sci. 17:808-813.
  25. Tamada, J., H. Yokota, M. Ohshima and I. Choi. 1996. Effect of application of cellulase and lactic acid bacteria inoculant on the fermentation quality of napier grass (Pennisetum purpureum Schum.) silage. Proc. 8th AAAP Anim. Sci. Congr. Jap. Soc. Zootech. Sci. Tokyo Japan 2:256-257.
  26. Weinberg, Z. G., G. Ashbell and A. Azrieli. 1988. The effect of applying lactic acid bacteria in ensiling on the chemical and microbiological composition of vetch, wheat and alfalfa silages. J. Appl. Bacteriol. 64:1-7.
  27. Weinberg, Z. G., G. Ashbell and B. Horev. 1989. The effect of sorbic acid on loss reduction during storage of orange peels. J. Sci. Food Agric. 46:253-258.
  28. Woolford, M. K. 1975. Microbiological screening of food preservation, cold sterilants and specific antimicrobial agents as potential silage additives. J. Sci. Food Agric. 26:229-237. https://doi.org/10.1002/jsfa.2740260214

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