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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Development of Array-based Technology for Detection of HAV Using Gold-DNA Probes

  • Wan, Zhixiang (Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Wang, Yefu (Department of Biochemistry, University of Western Ontario) ;
  • Li, Shawn Shun-Cheng (Department of Biochemistry, University of Western Ontario) ;
  • Duan, Lianlian (Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Zhai, Jianxin (Department of Biotechnology, College of Life Sciences, Wuhan University)
  • Published : 2005.07.31
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Abstract

A sensitive method for detection of Hepatitis A virus (HAV) by utilizing gold-DNA probe on an array was developed. Amino- modified oligodeoxynucleotides at the 5' position were arrayed on an activated glass surface to function as capture probes. Sandwich hybridization occurred among capture probes, the HAV amplicon, and gold nanoparticle-supported oligonucleotide probes. After a silver enhancement step, signals were detected by a standard flatbed scanner or just by naked eyes. As little as 100 fM of HAV amplicon could be detected on the array. Therefore, the array technology is an alternative to be applied in detection of HAV due to its low-cost and high-sensitivity.

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References

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