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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification of Differentially Expressed Proteins in Imatinib Mesylate-resistant Chronic Myelogenous Cells

  • Park, Jung-Eun (Division of Molecular Genomic Medicine, College of Medicine, Seoul National University) ;
  • Kim, Sang-Mi (Division of Molecular Genomic Medicine, College of Medicine, Seoul National University) ;
  • Oh, Jong-K. (Division of Molecular Genomic Medicine, College of Medicine, Seoul National University) ;
  • Kim, Jin-Y. (Division of Molecular Genomic Medicine, College of Medicine, Seoul National University) ;
  • Yoon, Sung-Soo (Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Lee, Dong-Soon (Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Kim, Young-Soo (Division of Molecular Genomic Medicine, College of Medicine, Seoul National University)
  • Published : 2005.11.30
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Abstract

Resistance to imatinib mesylate (also known as Gleevec, Glivec, and STI571) often becomes a barrier to the treatment of chronic myelogenous leukemia (CML). In order to identify markers of the action of imatinib mesylate, we used a mass spectrometry approach to compare protein expression profiles in human leukemia cells (K562) and in imatinib mesylate-resistant human leukemia cells (K562-R) in the presence and absence of imatinib mesylate. We identified 118 differentially regulated proteins in these two leukemia cell-lines, with and without a $1\;{\mu}M$ imatinib mesylate challenge. Nine proteins of unknown function were discovered. This is the first comprehensive report regarding differential protein expression in imatinib mesylate-treated CML cells.

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References

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