Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Establishment of an Individual Identification System Based on Microsatellite Polymorphisms in Korean Cattle (Hanwoo)

  • Received : 2004.09.22
  • Accepted : 2005.01.15
  • Published : 2005.06.01
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Abstract

This study was conducted to establish an individual identification system comprising of 19 microsatellite markers located on different bovine autosomes. The markers were typed on 257 animals from five cattle breeds. In total, 112 alleles were detected from the genotyping of 19 microsatellite markers. The average heterozygosities ranged from 0.292 to 0.824 and the polymorphic information content (PIC) ranged from 0.274 to 0.817 in Hanwoo. We found that there were differences in allele frequencies in Hanwoo when compared with other cattle breeds. The calculated cumulative power of discrimination (CPD) was 99.999% when nine microsatellite loci were used for analysis in the individual identification system. Also the matching probability, the probability that two unrelated animals would show the same genotypes, was estimated to be $0.44{\times}10^{-9}$. Therefore, the nine markers used in this study will be used for individual identification in two million Hanwoo individuals.

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References

  1. Chen, G. H., X. S. Wu, D. Q. Wang, J. Qin, S. L. Wu, Q. L. Zhou, F. Xie, R. Cheng, Q. Xu, B. Liu, X. Y. Zang and O. Olowofeso. 2004. Cluster Analysis of 12 Chinese Native Chicken Populations Using Microsatellite Markers. Asian-Aust. J. Anim. Sci. 17(8):1047-1052.
  2. Cho, G. J. and B. W. Cho. 2004. Microsatellite DNA Typing Using 16 Marker for Parentage Verification of the Korea Native Horse. Asian-Aust. J. Anim. Sci. 17(6):750-754.
  3. Fries, R. and G. Durstewitz. 2001. Digital DNA signatures: SNPs for animal tagging. Nat. Biotechnol. 19:508. https://doi.org/10.1038/89213
  4. Hirano, T., S. Nakane, K. Mizoshita, H. Yamaguchi, M. Inoue-Murayama, T. Watanabe, W. Barendse and Y. Sugimoto. 1996. Characterization of 42 highly polymorphic bovine microsatellite markers. Anim. Genet. 1996 Oct. 27(5):365-368.
  5. Kim, K. S., J. H. Eum and C. B. Choi. 2001. Genetic Diversity of Korean Cattle using Microsatellite Analysis. Anim. Sci. Technol. Kor. 43 (5):599-608.
  6. Kim. T. H., D. H. Yoon, E. W. Park, H. Y. Lee, S. J. Oh, I. C. Cheong, T. Y. Thak, K. N. Kim and J. Y. Han. 2000. A study on Genotype Frequencies of the Bovine Melanocortin Receptor 1 (MC1R) in cattle Breeds. J. Anim. Sci. Technol. Kor. 42 (6):735-744.
  7. Loftus, R. T., O. Ertugrul, A. H. Harba, M. A. A. El-Barody, D. E. MacHugh, S. D. E. Park and D. G. Bradley. 1999. A microsatellite survey of cattle from a centre of origin: The Near East. Molecular Ecology 8, pp. 2015-2022.
  8. Miller, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cell. Nucleic Acids Research 16:1215 (Abstr.).
  9. Nei, M. 1972. Genetics distance between populations, Am. Nat. 106, pp. 283-292.
  10. Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583-590.
  11. SanCrostoval, M., G. Renald and Y. Amigues. 2000. Tracabilite individuelle des viandes bovinew a l'aide de marqueurse genetiques. INRA Prod. Anim. 13 (4):269-276.
  12. SAS. 2002. SAS/STAT User's guide, SAS Institute Inc., Cary, NC, USA.
  13. Seo, K. S., Y. M. Cho and H. K. Lee. 2000. Development of Network System for the Application of HACCP in Livestock Production Stage. Agrolnformatics J. 1 (2):1-4.
  14. Syvanen, A. C. 2001. Accessing genetic variation, Genotyping single nucleotide polymorphisms. Nat. Rev. Genet. 2:930-942. https://doi.org/10.1038/35103535
  15. Vignal, A., D. Milan, M. SanCrostobal and A. Eggen. 2002. A review on SNP and their use in animal genetics. Genet. Sel. Evol. 34:275-305.
  16. Yoon, D. H. 2002. Molecular Genetic Diversity and Development of Genetic Markers in Association with Meat Quality for Hanwoo (Korean Cattle).Ph. D. Thesis.

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