Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Transmission of Bovine $\beta-Casein/Human$ Lactoferrin Fusion Gene in Transgenic Cattle

  • Han Yong-Mahn (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience & Biotechnology(KRIBB)) ;
  • Koo Deog-Bon (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience & Biotechnology(KRIBB)) ;
  • Park Jung-Sun (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience & Biotechnology(KRIBB)) ;
  • Kim Young-Hun (Doosan Development Business Group) ;
  • Lee Kea-Joung (Doosan Development Business Group) ;
  • Lee Kyung-Kwang (Doosan Development Business Group)
  • Published : 2005.12.01
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Abstract

This study was conducted to test whether the transgenic cattle pass the transgene to their progeny through germ cells, and whether the transgene is expressed in the mammary gland of ransgenic cows. Two male ransgenic calves were born from IVF-derived embryos injected with bovine $\beta-casein/human$ lactoferrin fusion gene and then grew up to be reproducible. Semen was collected from a transgenic bull after 18 mon of age and then frozen. Bovine oocytes matured in vitro were fertilized with spermatozoa of the transgenic bull and cultured in $50\;{\mu}L$ drops of CRlaa medium supplemented with 3 mg/mL BSA. After 48 h of culture, cleaved embryos were determined for the presence of transgenes by DNA polymerase chain reaction (PCR). Proportion of transgene positives among bovine embryos fertilized with sperm of the transgenic bull was $20.9\%$ (28/134). One of transgenic bulls did not produce transgenic sperm. Out of 34 calves produced from recipient heifers inseminated with semen of the other bull, 3 $(8.8\%)$ were transgenic animals (2 females and 1 male). Thus, one transgenic bull showed a low transmission frequency below Mendelian levels in both the IVF-derived embryos and his progeny. It was demonstrated by Southern blot that copy numbers of the transgene in the transgenic progeny enhanced about 1.8 times as compared to those of the founder bull The results demonstrate that the transgenic bull carrying human lactoferrin gene could pass his transgene to the progeny through germ cells, although he is a germ-line mosaic.

Keywords

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