Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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In Vitro Maturation of Round Spermatids Using Porcine Oviduct Epithelial Cell Monolayer Condition Medium

  • Jabed Md. Anower (Division of Life Science, College of Natural Science, Dong-Eui University) ;
  • Kamal Tania (Division of Life Science, College of Natural Science, Dong-Eui University) ;
  • Lee Seung-Min (Division of Life Science, College of Natural Science, Dong-Eui University) ;
  • Kim Byung Ki (Division of Life Science, College of Natural Science, Dong-Eui University)
  • Published : 2005.12.01
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Abstract

Porcine oviduct epithelial cells (POEC) are widely used in co-culture experiments to improve early embryonic development, in vitro fertilization in embryo transfer programs for domestic animals and in vitro maturation of immature germ cells. POEC were mechanically isolated and cultured in tissue culture medium 199. Cells grew continuously, and confluent monolayers were formed after 7 days. After forming confluent monolayer of epithelial cells, supernatant was collected as the condition medium for maturing round spermatids in vitro. Round spermatids were also separated mechanically and cultured in the POEC condition medium. In this study we observed that $20\%$ of round spermatid cultured were matured into elongating spermatid after 24 h, and about $10\%$ of round spermatid cultured showed complete elongation (elongated spermatid) within $24\~48$ h of in vitro culture. No further development was observed within $50\~72$ h and transformed cells lost their viability after 72 h. These preliminary findings suggest that the condition medium from POEC may be possible to overcome the round spermatid block by improving the milieu of culture system.

Keywords

References

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