Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Development of Porcine Embryos Following Intracytoplasmic Sperm Injection I. Effect of Activation and Sperm Capacitation

ICSI에 의한 돼지 수정란의 발달 I. 난자의 활성화와 정자의 수정능력 획득 유기 효과

  • Moon S. J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Ahn S. J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Kang M. J. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Kim K. H. (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
  • 문승주 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 안승주 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 강만종 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부) ;
  • 김광현 (전남대학교 농업생명과학대학 농업과학기술연구소 동물자원학부)
  • Published : 2005.12.01
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Abstract

This study was conducted to investigate the effects of oocyte activation after ICSI and of capacitation of insemination sperm before ICSI in Swine. There was no significant difference on cleavage rate and blastocyst developmental rate treated with ethanol, cycloheximide, or ethanol and cycloheximide jointly between treatment and control groups. However, significantly difference was found on cleavage rate and blastocyst developmental rate treated with caffeine and Ca-ionophore on capacitation of insemination sperm before ICSI (p<0.05). There was no significant difference on pronuclear formation rate and total oocyte activation rate treated with oocyte activation after ICSI between treatment and control groups, but was significant difference on pronuclear formation rate and total oocyte activation rate treated with capacitation treat of sperm (p<0.05).

본 연구는 ICSI후 돼지 난자의 활성화 처리와 ICSI전 주입정자의 수정 능력 획득 유기효과를 구명하기 위하여 실시하였다. ICSI후 ethanol, cycloheximide 그리고 ethanol과 cycloheximide를 병용처리 시 난할율과 배반포배 발달율이 대조구와 처리 구간에 유의적인 차이가 없었다(p<0.05). 그러나 ICSI전 caffeine과 Ca-ionpphre로 주입정자의 수정능력 획득 유기 처리 시 난할율과 배반포배발달율 모두 처리구에서 유의적으로 놓았다(p<0.05). 한편 ICSI후 난자활성화처리 시 전핵형성율과 총 난자 활성화율은 대조구와 처리구간에 유의차가 없었지만(p<0.05) 정자의 수정능력 획득 유기 처리 시 전핵형성율과 총 난자 활성화율은 처리구에서 유의적으로 높게 나타났다(p<0.05).

Keywords

References

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