한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제33권4호
  • /
  • Pages.315-318
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  • 2005
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

우모분해세균 Bacillus megaterium F7-1에 의한 단백질 분해효소 생산에 영향을 미치는 배양조건

Cultural Conditions for Pretense Production by a feather-Degrading Bacterium, Bacillus megaterium F7-1

  • Son Hong-Joo (Department of Biotechnology, Miryang National University)
  • 발행 : 2005.12.01
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초록

The effects of inorganic salts and feather concentrations on pretense production by Bacillus megaterium F7-1 were investigated. Pretense production was dependent on the presence of phosphates in the medium. Supplementation of medium with calcium ion slightly increased protease production. The highest protease production was obtained at $1.4\%$ feather. The optimal medium contained $2.0\%$ glucose, $0.8\%$ skim milk, $0.06\%\;K_{2}HPO_{4}\%,\;0.04\%\;KH_{2}PO{4},\;0.06\%\;NaCl,\;0.03\%\;MgCl_{2}\cdot6H_{2}O,\;0.002\%\;CaCl_{2}\cdot2H_{2}O,\;and\;1.4\%$ whole feather. By using this optimized medium, increased production of the protease was achieved compared with the cases of using basal medium.

키워드

참고문헌

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