Risk Assessment of Soil through Earthworm Toxicity Test of Nonylphenol and Bisphenol A

Nonylphenol과 Bisphenol A의 지렁이 독성시험 및 토양 중 생태 위해성평가

  • Lee Chul Woo (Environmental Exposure Assessment Divison, National institute of Environmental Research) ;
  • Park Soo Young (Environmental Exposure Assessment Divison, National institute of Environmental Research) ;
  • Yun Jun Heon (Environmental Exposure Assessment Divison, National institute of Environmental Research) ;
  • Choi Kyung Hee (Environmental Exposure Assessment Divison, National institute of Environmental Research) ;
  • Chung Young Hee (Environmental Exposure Assessment Divison, National institute of Environmental Research) ;
  • Kim Hyun Mi (Environmental Exposure Assessment Divison, National institute of Environmental Research)
  • 이철우 (국립환경과학원 환경노출평가과) ;
  • 박수영 (국립환경과학원 환경노출평가과) ;
  • 윤준헌 (국립환경과학원 환경노출평가과) ;
  • 최경희 (국립환경과학원 환경노출평가과) ;
  • 정영희 (국립환경과학원 환경노출평가과) ;
  • 김현미 (국립환경과학원 환경노출평가과)
  • Published : 2005.12.01

Abstract

Earthworm (Eisenia fetida) acute toxicity test was carried out and ecological risk assessment in soil was performed with national monitoring data. 14 day - $LC_{50}$ of nonylphenol and bisphenol A were 288.1 mg/kg and 90.1 mg/kg, respectively. And NOECs of nonylphenol and bisphenol A were 250 mg/kg and 50 mg/kg, respectively. Significant weight decrement was appeared at 70 mg/kg of bisphenol A, however, nonylphenol at concentrations tested did not severe adverse effect on the weight decrement. The environmental monitoring has been carrying out by NIER since 1999. Exposure levels of nonylphenol in soil were ND$\sim$10.55 $\mu$g/kg and those of bisphenol A were ND$\sim$15.50$\mu$g/kg in National Monitoring data which had been performed from 2000 to 2004. The measured soil exposure level was applied to evaluate the environmental risk assessment. The values of PNEC for bisphenol A and nonylphenol were determined as 0.5 mg/kg and 2.5 mg/kg, respectively using the safety factors which were suggested in EU and OECD. The values of HQ (PEC/PNEC) were determined to be below I for bisphenol A and nonylphenol when the maximum exposure levels for bispheol A (15.50$\mu$g/kg) and nonylphenol (10.55$\mu$g/kg) were applied. Conclusively, the environmental risk assessment of bisphenol A and nonylphenol was not critical in soil.

Keywords

References

  1. 국립환경연구원고서 제1998-41호. '화학물질유해성시험 연구기관의 지정에 관한 규정' (1998년 12월 23일)
  2. Bennie DT, Sullivan CA, Lee H-B and Maguire RJ. Alkylphenol polyethoxylate metabolites in Canadian sewage treatment plant waste streams, Water Qual Res J Can 1998; 33: 231-252
  3. Borras M, Laios I. Khissiin AE, Seo H-S, Lempereur F, Legros N and Leclercq G. Estrogenic and antiestrogenic regulation of the half-life of covalently labeled estrogen receptor in MCF-7 breast cancer cells, J steroid Biochem Molec Biol 1996; 57: 203-213 https://doi.org/10.1016/0960-0760(95)00272-3
  4. Environment Canada. Canadian environmental quality guidelines for nonylphenol and its ethoxylates (water, sediment and soil), Science-Based Solutions, August 2002; Report No. 1-3: 10-38
  5. European Union Risk Assessment Report. 4,4'-isopropylidenediphenol (bisphenol A), EC Joint Research Center 2003; 37: 42-93
  6. Hawrelak M, Bennett E and Metcalf C. The environmental fate of the primary degradation products of alkylphenol ethoxylate surfactants in recycled paper sludge, Chemosphere; 39: 745-752 https://doi.org/10.1016/S0045-6535(99)00010-7
  7. Krogh PH, Holmstrip M, Jensen J and Peterson SO. Ecotoxicological assessment of sewage sludge in agricultural soil. Denmark, Danish Environmental Protection Agency 1997; Report No 69: 53
  8. Lee H-B and Peart TE. Determination of 4-nonylphenol in effluent and sludge from sewage treatment plants, Anal Chem 1995; 67: 1976-1980 https://doi.org/10.1021/ac00109a011
  9. Lee H-B, Peart TE, Bennie DT and Maguire RJ. Determination of nonylphenol polyethoxylates and their carboxylic acid metablites in sewage treatment plant sludge by supercritical carbon dioxide extraction. J Chromatogr 1997; 785: 385-394 https://doi.org/10.1016/S0021-9673(97)00384-1
  10. Maria DF, Ekain C, Marfa MV, Arantzazu U, Mar B, Javier P and Jose VT. Ecological risk assessment of contaminated soils through direct toxicity assessment, Ecotoxicology and Environmental Safety 2005, 62: 174-184 https://doi.org/10.1016/j.ecoenv.2004.11.013
  11. Neuhauser EF, Loehr RC, Malecki MR, Milligan DL and Durkin PR. Toxicity of selected organic chemicals to the earthworm Eisenia fetida, J Environ Qual 1985; 14: 383-388 https://doi.org/10.2134/jeq1985.00472425001400030015x
  12. OECD. Draft report from the 2nd OECD expert consultation on Endocrine Disruptors Testing in Fish (EDF2), Tokyo, 15-16th March 2000, Test Guidelines Programme, Endocrine Disruptors Testing and Assessment Task Force
  13. OECD guidelines for the testing of chemicals 207, 'Earthworm, acute toxicity tests' (4 April 1984)
  14. Ren L, Marquardt MA and Lech JJ. Estrogenic effects of nonylphenol on pS2, ER and MUCI gene expression in human breast cancer cells MCF-7, Chemco- Biological Interactions 1997; 104: 55-64 https://doi.org/10.1016/S0009-2797(97)03767-8
  15. Renner R. European bans on surfactants trigger tranatlantic debate, Env. Sci. and Tech 1997; 31: 316-320 https://doi.org/10.1021/es972366q
  16. Rogers IH, Birtwell IK and Kruzynski GM. Organic extractables in municipal waste water, Vancouver, British Columbia, Water Pollut Res J Can 1986; 21: 187-204
  17. Singleton DW, Feng Y, Chen Y, Busch SJ, Lee AV, Puga A and Khan SA. Bisphenol A and estradiol exgert novel gene regulation in human MCF-7 derived breast cancer cells, Molecular and Cellular Endocrinology 2004: 221: 47-55 https://doi.org/10.1016/j.mce.2004.04.010
  18. Susana L, Amadeu M and Antonio JA. Terrestrial avoidance behaviour tests as screening tool to assess soil contamination, Environmental Pollution 2005; 138: 121-131 https://doi.org/10.1016/j.envpol.2005.02.013