한국식품저장유통학회지 (Food Science and Preservation)

  • 제12권6호
  • /
  • Pages.591-599
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  • 2005
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
권호 표지

썸머파워 양배추 추출물의 기능적 특성 최적화

Optimization for Functional Properties of Cabbage Extracts

  • 김현구 (한국식품연구원) ;
  • 도정룡 (한국식품연구원) ;
  • 홍주헌 (대구신기술사업단 전통생물소재산업화센터) ;
  • 이기동 (대구신기술사업단 전통생물소재산업화센터)
  • 발행 : 2005.12.01
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초록

본 실험은 반응표면분석법을 이용하여 양배추(썸머파워)의 추출조건에 따른 이화학적 특성을 평가하였다. 중심합성계획법에 따라 시료에 대한 용매비($10\~30$mL/g), 에탄올 농도($0\~100\%$) 및 추출온도($35\~95^{\circ}C$)를 달리하였을 때 회귀식의 $R^2$는 추출수율, 전자공여작용, tyrosinase 저해효과 및 SOD유사활성에서 각각 0.8162(p<0.1), 0.8173(p<0.1), 0.9374(p<0.01)와 0.9116(p<0.05)로 분석되었다. 조건별 추출물의 수율, 전자공여능, SOD유사활성에 대한4차원 반응표면을 superimposing하여 얻은 최적 추출조건 범위는 시료에 대한 용매비 $15\~30$ mL/g, 에탄을 농도$40\~80\%$, 추출온도 $50\~90^{\circ}C$로 나타났다. 최적 추출조건 범위내의 임의의 조건인 시료에 대한 용매비 25 mL/g, 에탄을 농도 $50\%$ 및 추출온도 $70^{\circ}C$을 각각의 회귀식에 대입하여 얻은 예측값은 추출물의 수율 $37.51\%$, 전자공여능 $45.66\%$, tyrosinase 저해효과 $53.59\%$, 아질산염 소거능의 경우 $73.30\%$(pH 1.0), $65.06\%$(pH 3.0), $16.41\%$(pH 4.2) 및 SOD 유사활성 $31.01\%$로 나타났다.

This study was conducted to optimize the extraction conditions from cabbage by a response surface methodology. In extraction conditions based on the central composite design with variations, the ratio of solvent to sample ($10\~30$mL/g), ethanol concentration ($0\~100\%$) and extraction temperature ($35\~95^{circ}C$) coefficients of determinations ($R^2$) were 0.8162(p<0.1), 0.8173(p<0.1), 0.9374(p<0.01) and 0.9116(p<0.05) in extraction yield, electron donating ability, tyrosinase inhibition and SOD-like ability, respectively. Estimated extraction conditions for the maximizing yield, electron donating ability and SOD-like ability were $15\~30$ mL/g in ratio of solvent to sample, $40\~80\%$; ethanol concentration, and $50\~90^{\circ}C$ ; extraction temperature. Predicted values at the optimum condition (25 mL/g solvent to sample, $50\%$ ethanol concentration and $70^{\circ}C$ in extraction temperature) were in good agreement with observed values.

키워드

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