Cytotoxicity of Copper Nanoparticles in Cultured Human Bronchial Epithelial Cells (BEAS-2B)

구리로 만든 나노입자의 기관지상피세포에 미치는 독성

  • Park Eun-Jung (College of Pharmacy, Dongduk Women's University) ;
  • Park Kwangsik (College of Pharmacy, Dongduk Women's University)
  • 박은정 (동덕여자대학교 약학대학) ;
  • 박광식 (동덕여자대학교 약학대학)
  • Published : 2005.12.01


Nanomaterials, which ranges in size from 1 to 100 nm, have been used to create uqnique devices at the nanoscale level possessing novel physical and chemical functional properties. However, the toxicities of nanomaterials have not been fully tested and the risk of nanomaterials is emerging issues in these days. In this study, the cytotoxicity of copper nanoparticles was tested in cultured human bronchial epithelial cells. As a results, copper nanoparticles showed cytotoxicity similar with cupric ion and the apoptotic mechanisms of DNA fragmentation and caspase-3 activation were involved. Induction of heme oxygenase-1 and thioredoxin reductase by copper nanoparticles indicated that cytotoxicity of copper nanoparticles is likely to be mediated through oxidative stress.



  1. Angeline, S.A., Amy, J.W., Aaron, B., Kaili, A.T., Lihda, K., Nicole, V.S., Kimberley, A.O. and Joshua, W.H. (2003): Genomic and proteomic profiling of responses to toxic metals in human lung cells. Toxicogenomics, 3, 825-838
  2. Anne, B.G., Matthew, E.J. and Hajine, N. (2005): Thioredoxin: friend or foe in human disease? TRENDS in Pharmacological Sciences, 26, 398-404
  3. Choi, J.H., Kim, J.S., Kim, Y.C., Kim, Y.S., Chung, N.H. and Cho, M.H. (2004): Comparative study of PM2.5- and PM10-induced oxidative stress in rat lung epithelial cell. Joumal of Veterinary Science, 5, 11-18
  4. Cui, D. and Gao, H. (2003): Advance and prospect of bionanomaterials. Biotechnol. Prog., 19, 683-692
  5. Feynman, R. (1991): There is plenty of room at the bottom. Science, 254, 1300-1301
  6. Holsapple, M.P., Farland, W.H., Landry, T.L., Monteiro-Riviere, N.A., Carter, J.M., Walker, N.J. and Thomas, K.V. (2005): Research strategies for safety evaluation of nanomaterials, Part II: Toxicological and sfety evaluation of nanomaterials, current challenges and data needs. Toxicological Sci., 88, 12-17
  7. Hsiao, W, Mo, Z.Y., Fang, M., Shi, X.M. and Wang, F. (2000): Cytotoxicity of $PM_{2.5}$ and $PM_{2.5}_{-10}$ ambient air pollutants assessed by the MTI and the Comet assays. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 471, 45-55
  8. Janet, Y.U. and Carl, L.K. (2005): Copper, oxidative stress, and human health. Molecular Aspects of Medicine, 26, 268-298
  9. Lee, C., Na, J.G, Lee, K.C. and Park, K. (2002): Choriogenin mRNA induction in male medaka Oryzias latipes as a biomarker of endocrine disruption. Aquatic Toxicology, 61, 233-242
  10. Lisa, M.G. and Ching, K.C. (2003): Copper toxicity, oxidative stress, and antioxidant nutrients. Toxicology, 189, 147-163
  11. Mahin, D.M. and Peter, E.M. (2005): 30 som years of heme oxygenase: From a 'molecular wecking ball' to a 'mesmerizing' trigger of cellular events, Biochemical and Biophysical Research Communications
  12. Mazzola, L. (2003): Commercializing nanotechnology. Nature Biotechnology, 21, 1137-1143
  13. Oberdorster, G., Overdorster, E. and Oberdorster, J. (2005): Nanotoxicology, An emerging discipline evolving from studies of ultrafine particles. Environ. Health Perspect, 113, 823-839
  14. Paolo, S., Silvano, G., Paola, A., Patrizia, F., Valeria, M., Sabina, N., Pierfranco, R., Giorgio, G., Alberto, B., Franco, P., Antonio, B. and Claudio, B. (2004): Carvedilol prevents doxorubicin- induced free radical release and apoptosis in cardiomyocytes in vitro. Joumal of Molecular and Cellular Cardiology, 37, 837-846
  15. Pattie, S.G. and Christiaan, L. (2002): Mitochondrial dysfunction is an early indicator of doxorubicin-induced apoptosis. Biochimica et Biophysica Acta, 1588, 94-101
  16. Ryu, J., Lee, M.S., Na, J.G., Chung, K., Song, B.J. and Park, K. (2004): Molecular cloning of CYP1A cDNA of medaka (Oryzias latipes) and regulation by environmental pollutants. Environ. Toxicol. Chem., 23, 1004-1011
  17. Salata, O.V. (2004): Applications of nanopaticles in biology and medicine. J. Nanobitotech., 2, 3-9
  18. Thomas, K. and Sayre, P. (2005): Research strategies for safety evaluation of nanomaterials. Art II: Toxicological Sci., 87, 316-321