Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Dietary Protein and Calcium Levels on Calcium Metabolism of the Rat

식이단백질과 칼슘 수준이 흰쥐의 칼슘대사에 미치는 영향

  • 윤군애 (동의대학교 식품영양학과) ;
  • 황혜진 (동의대학교 식품영양학과)
  • Published : 2005.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to examine effect dietary protein and calcium levels on calcium metabolism of the rat. Weaned 6-week old male rats were divided into 4 groups and were fed experimental diets for six weeks. Experimental groups were HPNC group-high protein normal calcium (protein: 400 g/kg diet, calcium: 0.5%), HPLC group-high protein low calcium (protein: 400 g/kg diet, calcium: 0.1%), NPNC group-normal protein normal calcium (protein: 200 g/diet, calcium: 0.5%), NPLC group-normal protein low calcium (protein: 200 g/diet, calcium: 0.1%). The calcium excretion in urine was higher in high protein group than in normal protein group, and it was highest in HPLC group. The activation of alkaline phophatase had a tendency to low in normal calcium group, and the concentration of parathyroid hormone (PTH) was the lowest in HPLC group. The deoxypyridinoline (DPD) concentration of urine was investigated as the highest in HPLC group and it was significantly lower in HPNC group that consumed normal calcium. The bone density of the femur was the highest in NPNC group and the lowest in NPLC group. As the results of this study, calcium excretion in urine and DPD density were the highest and the bone density was the lowest in HPLC group. It may suggest that the deficiency of calcium causes adversely effect in calcium metabolism upon consuming high protein diet. Therefore, it should be emphasized to consume enough calcium to prevent the hindrance of skeletal metabolism caused by deficiency of calcium upon consuming high protein diet.

흰쥐를 대상으로 하여 식이 단백질 수준에 따라 칼슘 수준을 달리하여 식이를 공급하여 칼슘 식이효율, 칼슘대사와 관련된 호르몬, 대퇴골의 무게 및 칼슘 함유량, 골밀도를 측정하였다. 실험 식이의 구성은 \circled1 HPNC군-고단백 적정칼슘군(Protein: 400 g/kg diet, calcuim: 0.5%) \circled2 HPLC군-고단백 저칼슘군(Protein: 400 g/kg diet, calcium: 0.1%) \circled3 NPNC군-적정 단백 적정 칼슘군 protein: 200 g/kg diet, calcium: 0.5%) \circled4 NPLC군-적정단백 저칼슘군(protein: 200 g/kg diet, calcium: 0.1%)으로 구별하였다. 실험 종료까지의 체중은 NPLC군에서 가장 많이 증가하였으나, 실험군간의 차이를 나타내지 않았고, 식이 섭취량도 실험군간에 차이를 보이지 않았다. 뇨 칼슘배설량은 고단백군이 적정단백군보다 높은 경향을 나타내었고, HPLC군에서 유의적으로 높은 값을 보였으며 체내 칼슘보유량과 흡수율은 실험군에 따른 차이가 없었다. 뼈형성의 biomarker인 ALP의 활성은 저칼슘 식이군인 NPLC군에서 유의하게 높았고, 고단백 적정칼슘을 섭취한 HPNC군에서 유의적으로 낮았다. 혈액의 PTH 농도는 HPLC군에서 가장 낮게 나타났으며, 소변의 DPD농도는 저칼슘 식이군인 HPLC군과 NPLC군에서 높은 수치로 관찰되었고, HPNC군에서는 유의적으로 낮아졌다. 대퇴골의 건조 전의 습윤무게는 중 100 g당의 무게로 환산했을 때 NPLC군에서 가장 낮았으며 건조 후의 대퇴골의 무게 역시 다른군에 비해 NPLC군에서 가장 낮았다. 대퇴골의 회분 함량은 실험군간의 큰 차이를 나타내지 않았고, 칼슘 함량은 NPLC군과 HPLC군에서 유의하게 낮았다. 대퇴골의 골밀도는 NPNC군에서 가장 높게 나타났으며, NPC군은 가장 낮게 나타났다. 본 연구결과 고단백 저칼슘 식이 섭취시 요중 칼슘 배설량이 가장 많았고, DPD 농도가 다른 군에 비하여 유의적으로 높았으며, 골밀도도 가장 낮게 조사되어 고단백식이 섭취시 칼슘섭취 부족은 칼슘대사에 좋지 못한 결과를 나타냄을 알 수 있었다.이로 볼 때 적절한 단백질과 칼슘 섭취가 골격의 건강을 유지하고 노령화에 따른 골격질환을 예방할 수 있고, 특히 고단백 섭취시에는 칼슘영 양이 부족할 경우 골격 대사를 저해할 수 있으므로 충분한 칼슘 섭 취가 무엇보다도 중요하다고 본다.

Keywords

References

  1. Lawrence GR, Smith JA. 1989. Pathogenesis, prevention and treatment of osteoporosis. Ann Rev Med 40: 251-267 https://doi.org/10.1146/annurev.me.40.020189.001343
  2. Heaney RP. 1993. Nutritional factors in osteoporosis. Ann Rev Nutr 13: 287-316 https://doi.org/10.1146/annurev.nu.13.070193.001443
  3. Smith DM, Nance WE, Kang KW, Christiansen JC, Johnston SS Jr. 1973. Genetic factors in determining bone loss. J Clin Invest 52: 2800-2808 https://doi.org/10.1172/JCI107476
  4. Calvo MS. 1993. Dietary phosphorus, calcium metabolism and bone. J Nutr 123: 1627-1633
  5. Wachman A, Bernstein DS. 1968. Diet and osteoporosis. Lancet 1: 958-959 https://doi.org/10.1016/S0140-6736(68)90908-2
  6. Allen LH, Eood RJ. 1994. Calcium and phosphorus. In Modern Nutrition in Health and Disease. 8th ed. Shills ME, Olson JA, Shike M, eds. Lea & Febiger, Philadelphia. p 144-163
  7. Healney RP, Weaver CM, Fitzsimmons ML. 1990. The influence of calcium load on absorption fraction. J Bone Miner Res 11: 1135-1143
  8. Ira W, Taylor N, Dorothy J, Sara B. 1994. Calcium nutrition in the elderly. In Handbook of nutrition in the aged. 2nd ed. Ronald RW, ed. CRC Press, New York. p 355-363
  9. Kim WY, Moon KW, Kim JH. 1995. A study on the longterm effects of dietary level on Ca and skeletal metabolism in ovariectomized rats. Korean J Nutr 27: 415-425
  10. Linkswiler HM, Zemel MB, Hegsted M, Schuette S. 1981. Protein-induced hypercalciuria. Fed Proc 40: 2429-2433
  11. Delmi M, Rapin CH, Bengoa JM, Delmas PD, Vasey H, Bonjour JP. 1990. Dietary supplementation in elderly patients with fractured neck of the femur. Lancet 335: 1013-1016 https://doi.org/10.1016/0140-6736(90)91073-J
  12. Schurch MA, Rizzoli R, Slosman D, Vadas L, Vergnaud P, Bonjour JP. 1998. Protein supplements increase serum insulin-like growth factor-I levels and attenuate proximal femur bone loss in patients with recent hip fracture. Ann Intern Med 128: 801-809 https://doi.org/10.7326/0003-4819-128-10-199805150-00002
  13. Ministry of Health and Welfare. 1999. '98 National Nutrition Survey Report'
  14. Chung SH, Choi MJ. 1995. Effect of dietary protein level on Ca efficiency in bone mineral density in growing rats. Korean J Nutr 28: 817-824
  15. Barzel US, Massey LK. 1998. Excess dietary protein can adversely affect bone. J Nutr 128: 1051-1053
  16. Kerstetter JE, Mitnick ME, Gundberg CM, Caseria DM, Ellison AF, Carpenter TO, Insogna KL. 1999. Changes in bone turnover in young women consuming different levels of dietary protein. J Clin Endocrinol Metab 84: 1052-1055 https://doi.org/10.1210/jc.84.3.1052
  17. Shapses SA, Robins SP, Schwartz EI, Chowdhury H. 1995. Short-term changes in calcium but not protein intake alter the rate of bone resorption in healthy subjects as assessed by urinary pyridinium cross-link excretion. J Nutr 125: 2814-2821
  18. Lee CK, Choi JS, Jeon YJ, Byun HG, Kim SK. 1997. The properties of natural hydroxyapatite isolated from tuna bone. Bull Korean Fish Soc 30: 652-659
  19. Aloia JF, Cohr SH, Vaswani A, Yeh JK, Yuen K, Ellis K. 1985. Risk factors for postmenopausal osteoporosis. Am J Med 78: 95-100
  20. Price PA, Pathermore JG, Doftos LJ. 1980. New biochemical marker for bone metabolism. J Clin Invest 66: 878-883 https://doi.org/10.1172/JCI109954
  21. Delmas PD, Hardy P, Garnero P, Dain M. 2000. Monitoring individual response to hormone replacement therapy with bone marker. Bone 26: 553-560 https://doi.org/10.1016/S8756-3282(00)00271-4
  22. Dawson-Hughes B, Harris SS. 2002. Calcium intake influences the association of protein intake with rates of bone loss in elderly men and women. Am J Clin Nutr 75: 773-779 https://doi.org/10.1093/ajcn/75.4.773
  23. Meyer HE, Pedersen JI, Loken EB, Tverdal A. 1997. Dietary factors and the incidence of hip fracture in middle-aged Norwegians. Am J Epidemiol 145: 117-123 https://doi.org/10.1093/oxfordjournals.aje.a009082
  24. Heaney RP. 2002. Protein and calcium: antagonists or synergists? Am J Clin Nutr 75: 609-610 https://doi.org/10.1093/ajcn/75.4.609

Cited by

  1. Consumption of health functional food and dietary habits, nutrient intake and dietary quality of college students in Incheon vol.46, pp.2, 2013, https://doi.org/10.4163/kjn.2013.46.2.166
  2. 주정과 구연산 및 식이성 칼슘소재를 처리한 멸치분말이 흰쥐의 칼슘대사에 미치는 영향 vol.35, pp.7, 2005, https://doi.org/10.3746/jkfn.2006.35.7.860