The Korean Journal of Applied Statistics (응용통계연구)

The Korean Statistical Society (한국통계학회)
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Print-tip Normalization for DNA Microarray Data

DNA 마이크로어레이 자료의 PRINT-TIP별 표준화(NORMALIZATION) 방법

  • Yi Sung-Gon (Department of Statistics, Seoul National University) ;
  • Park Taesung (Department of Statistics, Seoul National University) ;
  • Kang Sung Hyun (Department of Statistics, Seoul National University) ;
  • Lee Seung-Yeaun (Department of Applied Mathematics, Sejong University) ;
  • Lee Yang Sung (Department of Biochemistry, Hanyang University)
  • 이성곤 (서울대학교 자연과학대학 통계학과) ;
  • 박태성 (서울대학교 자연과학대학 통계학과) ;
  • 강성현 (서울대학교 자연과학대학 통계학과) ;
  • 이승연 (세종대학교 응용수학과) ;
  • 이용성 (한양대학교 생화학교실)
  • Published : 2005.03.01
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Abstract

DNA microarray experiments allow us to study expression of thousands of genes simultaneously, Normalization is a process for removing noises occurred during the microarray experiment, Print-tip is regarded as one main sources of noises, In this paper, we review normalization methods most commonly used in the microarray experiments, Especially, we investigate the effects of print-tips through simulated data sets.

DNA마이크로어레이 기술은 수천 개 또는 수만 개의 유전자의 발현을 동시에 탐색할 수 있는 새로운 과학 기술이다. 표준화(normalization)는 마이크로어레이 실험에서 다양한 원인에 의해 발생하는 잡음(noise)을 줄이거나 제거하는 과정을 나타낸다. print-tip의 변동은 잡음의 주요 요인으로 인지되어 왔다. 본 논문에서는 잡음의 주요 발생요인이 되는 print-tip의 변동을 조절하기 위한 print-tip 표준화 작업에 대한 객관적인 비교 및 그 타당성 평가를 하였다. 먼저 그동안 제안된 여러 표준화 방법들 중에서 가장 널리 사용되고 있는 방법들을 정리해서 소개한 후에, 잡음이 많이 포함된 실제 cDNA 실험자료를 이용하여 각 표준화 방법의 특성을 비교해 보았다. 또한 실험자료와 유사한 모의분포를 생성한 후에 print-tip 표준화 작업에 대한 체계적인 비교를 해 보았다.

Keywords

References

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