Journal of the Optical Society of Korea

Optical Society of Korea (한국광학회)
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Lateral Resolution Enhancement in Confocal Self-interference Microscopy with Commercial Calcite Plate

  • Kang DongKyun (Nano Opto Mechatronics Lab., Dept. of Mechanical Engineering KAIST) ;
  • Yoo HongKi (Nano Opto Mechatronics Lab., Dept. of Mechanical Engineering KAIST) ;
  • Lee SeungWoo (Nano Opto Mechatronics Lab., Dept. of Mechanical Engineering KAIST) ;
  • Gweon Dae-Gab (Nano Opto Mechatronics Lab., Dept. of Mechanical Engineering KAIST)
  • Received : 2005.01.27
  • Published : 2005.03.01
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Abstract

In light microscopy, spatial resolution is limited by diffraction effect. Confocal microscopy has improved resolutions in both lateral and axial directions, but these are still limited by diffraction effect. Confocal self-interference microscopy (CSIM) uses interference between two perpendicularly polarized beams to enhance lateral resolution. In previous research, we proposed a calcite plate with its optic-axis perpendicular to the propagation angle and one of the boundary surfaces of the plate. This type of plate is not widely used to our knowledge. In this paper, we change the calcite plate to more common one, which is commercially available. This calcite plate has its optic axis in the plane of incidence. We analyze the characteristics of this calcite plate and numerically compare the performances of CSIM in previous research and CSIM with the commercial calcite plate. Numerical results show improved performance when using the commercial calcite plate

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References

  1. J. B. Pawley, Handbook of biological confocal microscopy (Plenum Press, New York, 1995)
  2. M. Vaez-lravani and D. 1. Kavaldjiev, 'Resolution beyond the diffraction limit using frequency-domain field confinement in scanning microscopy,' Ultramicroscopy vol. 61, pp. 105-110, 1995 https://doi.org/10.1016/0304-3991(95)00107-7
  3. C. J. R. Sheppard, 'Binary optics and confocal imaging,' Opt. Lett. vol. 24, no. 8, pp. 505-507, 1999 https://doi.org/10.1364/OL.24.000505
  4. M. A. A. Neil, R. Juskaitis, T. Wilson and Z. J. Laczik, 'Optimized pupil-plane filters for confocal microscope point- spread function engineering,' Opt. Lett. vol. 25, no. 4, pp. 245-247, 2000 https://doi.org/10.1364/OL.25.000245
  5. L. Liu, X. Deng, L. Yang, G. Wang, and Z. Xu, 'Effect of an annular pupil ftIter on differencial confocal microscopy,' Opt. Lett. vol. 25, no. 23, pp. 1711-1713, 2000 https://doi.org/10.1364/OL.25.001711
  6. S. W. Hell, M. Schrader, and H. T. M. Van Der Voort, 'Far- field fluorescence microscopy with three-dimentional resolution in the 100-nm range,' 1. Microsc.-Oxford, vol. 187, no. 1, pp. 1-7, 1997 https://doi.org/10.1046/j.1365-2818.1997.2410797.x
  7. D. Kang and D. Gweon, 'Enhancement of lateral resolution in confocal self-interference microscopy,' Opt. Lett. vol. 28, no. 24, pp. 2470-2472, 2003 https://doi.org/10.1364/OL.28.002470
  8. M. Born and E. Wolf, Principles of Optics (Cambridge University Press, UK, 1999)

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