Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Silkworm Extract on Disaccharidase Activities of Small Intestine and Blood Glucose-Lowering in C57BL/6J Mice

  • Ahn, Jung-Mo (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Hong, Jung-Hee (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Kim, Mi-Lim (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Lee, In-Seon (Traditional Microorganism Resources Center, Keimyung University) ;
  • Rhee, Soon-Jae (Department of Food Science and Nutrition, Catholic University of Daegu)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study examined the anti-diabetic effect of a silkworm extract in C57BL/6J mice, an ob/㏈ model, fed a high fat diet for 8 weeks. The body weight was not significantly different with the silkworm-extract supplement, nor did food intake and body weight gains also did not differ significantly among the high-fat diet groups. However, the water intake by the silkworm-extract supplemented groups increased significantly compared with that by the distilled-water supplement group, nonetheless, the FER did not differ significantly. For all groups, the blood glucose increased the most after 30 minutes and yet returned to a fasting level within 90 minutes. The fasting time and resulting glucose tolerance for the silkworm-extract supplemented groups were significantly decreased compared to that for the high fat diet with distilled water supplement group, while the level of blood glucose in silkworm-extract supplemented groups was significantly decreased compared with than in the diabetic control group. The HbA1c and insulin levels were no different among the groups. The sucrase and lactase activities in the proximal small intestine were significantly decreased in the silkworm-extract supplement groups compared to that in the diabetic control group. There was no significant difference in the glycogen contents in the liver and muscle among the groups. In conclusion, it was found that the silkworm-extract supplement repressed the disaccharidase activity in the small intestines mucosa of the C57BL/6J mice.

Keywords

References

  1. FNB (Food and Nutrition Board). 1974. Recommended dietary all owanles national academy of science
  2. Goodhart RB, Shills ME. 1976. Modem nutrition in health and disease. Lea and Febiger, Philadelphia. p 900-901
  3. Hutton B, Fergusson D. 2004. Changes in body weight and serum lipid profile in pbese patients treated with orlistat in addition to hypocaloric diet: a systematic review of randomized-clinical trials. Am J Clin Nutr 80: 1461-1468
  4. Nakatani K, Noma K, Nishioka J, Kasai Y, Morioka K, Katsuki A, Hori Y, Yano Y, Sumida Y, Wada H, Nobori T. 2005. Adiponectin gene variation associates with the increasing risk of type 2 diabetes in non-diabetic Japanese subjects. lnt J Mol Med 15: 173-137
  5. Skidmore PM, Yarnell JW. 2004. The obesity epidemic: prospects for prevention. QJM 97: 817-825 https://doi.org/10.1093/qjmed/hch136
  6. Williams SR, Anderson SL. 1995. Diabetes mellitus. In Diet theraphy. Mosby-Year Book, St. Louis. p 719-204
  7. Brownlee M. 1992. Glycation products and the pathogenesis of diabetic complications. Diabetes Care 15: 1835-1843 https://doi.org/10.2337/diacare.15.12.1835
  8. Yoo SK, Kim MJ, Kim JW, Rhee SJ. 2002. Effects of YK-209 mulberry leaves on disaccharidase activites of small intestine and blood glucose-lowering in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 31: 1071-1077 https://doi.org/10.3746/jkfn.2002.31.6.1071
  9. Hong JH, Park MR, Rhee SJ. 2002. Effects of YK-209 mulberry leaves on HMG-CoA reductase and lipid composition of liver in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 31: 826-833 https://doi.org/10.3746/jkfn.2002.31.5.826
  10. Kim SK. 1991. Bonchohak. Younglimsa, Seoul. p 598
  11. Rhee SJ, Kim MJ, Kwag OG. 2002. Effects of green tea catechin on prostaglandin synthesis of renal glomerular and renal dysfunction in streptozotocin-induced diabetic rats. Asia Pacific J Clin Nutr 11: 232-236 https://doi.org/10.1046/j.1440-6047.2002.00312.x
  12. Rhee SJ, Choi JH, Park MR. 2002. Green tea catechin improves microsomal phospholipase Az activity and the arachidonic acid cascade synstem in the kidney of diabetic rats. Asia Pacific J Clin Nutr 11: 226-231 https://doi.org/10.1046/j.1440-6047.2002.00303.x
  13. Choi SS. 2004. The effects of Alisma canaliculatumbutanol fraction with selenium on glycogen level, lipid metabolism and lipid peroxidation in streptozotocin-induced diabetic rats. Korean J Nutr 37: 15-22
  14. Lim SJ, Kim KJ. 1995. Hypoglycemic effect of Polygonatum odoranum var. pluriforum ohwi extract in streptozotocin-induced diabetic rats. Korean J Nutr 28: 727-736
  15. Jo YJ, Bang MA. 2004. Hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocin-induced diabetic rats. Korean J Nutr 37: 5-10
  16. Seo JK, Bang MA, Chun SS, Lee YY, Lee SJ, Shon MY, Sung NJ. 2004. Effect of physiologically active compounds isolated from platycodon grandiflorum on streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 33: 981-986 https://doi.org/10.3746/jkfn.2004.33.6.981
  17. Ji SD, Shin KH, AIm DK, Cho SY. 2003. The mass production technology and pharmacological effect of silkworm cordyceps Peacilomyces tenuipes. Food Sci Ind 36: 38-48
  18. Chung SH, Yu JH, Kim EJ, Ryu KS. 1996. Blood glucose lowering effect of silkworm. Bull Kyung Hee Pharma Sci 24: 95-100
  19. Shiomi S, Habu D, Takeda T, Nishiguchi S, Kuroki T, Tanaka T, Tsuchida K, Yamagami S. 1998. Significance of pepridoglycan on patients with chronic liver disease. J Nutr 122: 760-765
  20. Park IK, Lee JO, Lee HS, Seol KY, Ahn YJ. 1998. Cytotoxic activity of Bombyx mori and Morus alba derived materials against human tumor cell lines. Agric Chem Biotech 41: 187-190
  21. Ryu KS, Lee HS, Chung SH, Kang PD. 1997. An activity of lowering blood glucose levels according to preparative conditions of silkworm powder. Korean J Seric Sci 39: 79-85
  22. Dahlqvist A. 1974. Disaccharidase. In Method of enzymatic analysis? 2nd ed. Academic press, Stockholm. Vol 2, p 916
  23. Lowry OH, Roseborough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275
  24. Lo S, Russel CJ, Taylor AW. 1970. Determination of glycogen in small tissue. Appl Physiol 28: 234-236
  25. Sreel RGD, Tome JH. 1990. Principles and procedures of statistics. McGrow Hill, New York, USA
  26. Kim MS, Choue RW, Chung SH, Koo SJ. 1998. Blood glucose lowering effects of mulberry leaves and silkworm extract on mice fed with high-carbohydrate diet. Korean J Nutr 31: 117-125
  27. Lee YM, Kim H, Hong EK, Kang BH, Kim SJ. 2000. Water extract of 1:1 mixture of phellodendron cortex and Aralia cortex has inhibitory effects on oxidative stress in kidney of diabetic rats. J Ethnopharmacol 73: 429-436 https://doi.org/10.1016/S0378-8741(00)00302-0
  28. Seo SY, Kim HR. 1997. Effects of Aralia canescens and phellodendron amurense extracts on streptozotocin induced diabetic ICR mice. J Korean Soc Food Sci Nutr 26: 689-696
  29. Hanefield M, Schmechel H, Julius U. 1991. Five year incidence of coronary heart disease related to major risk factors and metabolic control in newly diagnosed noninsulin dependent diabetes. Nutr Metab 1: 135-140
  30. Ryu KS, Lee HS, Choue RW, Chung SH. 1997. Utilization and isolation of new active substances from sericulture related matarials. 40th Anniversary Commemoration symposium. Progress and future development of sericultural science & technology. Korean Society of Sericultural Science. p 133-158
  31. Bressler R, Johnson D. 1992. New pharmacological approaches to therapy of NIDDM. Diabetes Care 15: 797-805
  32. Kennedy DL, Piper JM, Baum C. 1988. Trends in use of oral hypoglycemic agent during 1964-1986. Diabetes Care 11: 558-562 https://doi.org/10.2337/diacare.11.7.558