Environmental Engineering Research

  • 제10권5호
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  • Pages.213-226
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  • 2005
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  • 1226-1025(pISSN)
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  • 2005-968X(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
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ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF AN IMMOBILIZED BACTERIUM PRODUCING N2 FROM NH4+ UNDER AN AEROBIC CONDITION

  • Park, Kyoung-Joo (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Cho, Kyoung-Sook (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Kim, Jeong-Bo (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Lee, Min-Gyu (Division of Applied Chemical Engineering, Pukyong National University) ;
  • Lee, Byung-Hun (Division of Environmental System Engineering, Pukyong National University) ;
  • Hong, Young-Ki (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Kim, Joong-Kyun (Division of Food Science and Biotechnology, Pukyong National University)
  • 발행 : 2005.10.31
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초록

To treat wastewater efficiently by a one-step process of nitrogen removal, a new bacterial strain producing $N_2$ gas from ${NH_4}^+$ under an aerobic condition was isolated and identified. The cell was motile and a Gram-negative rod, and usually occurred in pairs. By 16S-rDNA analysis, the isolated strain was identified as Enterobacter asburiae with 96% similarity. The isolate showed that the capacity of $N_2$ production under an oxic condition was approximately three times higher than that under an anoxic condition. Thus, the consumption of ${NH_4}^+$ by the isolate was significantly different in the metabolism of $N_2$ production under the two different environmental conditions. The optimal conditions of the immobilized isolate for $N_2$ production were found to be pH 7.0, $30^{\circ}C$ and C/N ratio 5, respectively. Under all the optimum reaction conditions, $N_2$ production by the immobilized isolate resulted in reduction of ORP with both the consumption of DO and the drop of pH. The removal efficiencies of $COD_{Cr}$, and TN were 56.1 and 60.9%, respectively. The removal rates of $COD_{Cr}$, and TN were the highest for the first 2.5 hrs with the removal $COD_{Cr}/TN$ ratios of 32.1, and afterwards the rates decreased as reaction proceeded. For application of the immobilized isolate to a practical process of ammonium removal, a continuous operation was executed with a synthetic medium of a low C/N ratio. The continuous bioreactor system exhibited a satisfactory performance at 12.1 hrs of HRT, in which the effluent concentrations of ${NH_4}^+$-N was measured to be 15.4 mg/L with its removal efficiency of 56.0%. The maximum removal rate of ${NH_4}^+$-N reached 1.6 mg ${NH_4}^+$-N/L/hr at 12.1 hrs of HRT(with N loading rate of $0.08\;Kg-N/m^3$-carrier/d). As a result, the application of the immobilized isolate appears a viable alternative to the nitrification-denitrification processes.

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