Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Antimicrobial Activities and Phenolic Compounds of Pyroligneous Liquor

목초액의 항균활성과 페놀화합물의 함량

  • Jong-Soo Kim (Department of Food Science & Technology, Kyungpook National University) ;
  • Seung-Woo Park (Gyeongsangbuk-Do Institute of Health and Environment) ;
  • You-Shik Ham (Ulsan Institute of Health and Environment) ;
  • Soo-Kun Jung (Ulsan Institute of Health and Environment) ;
  • Sang-Han Lee (Department of Food Science & Technology, Kyungpook National University) ;
  • Shin-Kyo Chung (Department of Food Science & Technology, Kyungpook National University)
  • 김종수 (경북대학교 식품공학과) ;
  • 박승우 (경상북도 보건환경연구원) ;
  • 함유식 (울산광역시 보건환경연구원) ;
  • 정수근 (울산광역시 보건환경연구원) ;
  • 이상한 (경북대학교 식품공학과) ;
  • 정신교 (경북대학교 식품공학과)
  • Published : 2005.10.01
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Abstract

Antimicrobial activities of pyroligneous liquor were investigated by determining Minimal Inhibitory Concentration (MIC). The solvent extracts of pyroligneous liquor, which were extracted by using solvents with different polarities such as hexane, ethylacetate, or butanol. The activities were examined by disc diffusion method using MIC against 7 food poisoning microbe type strains. Antimicrobial activities were shown in hexane, ethylacetate, butanol, and aqueous fractions of pyroligneous liquor. Among the four fractions, ethylacetate fraction showed the highest inhibitory effect on the microorganism such as Shigella sonnei, and Yersinia enterocolitica at the concentration of 2.0 mg/disc. The purified P-1 and P-2 fractions isolated by silica gel column chromatography from ethylacetate fraction of pyroligneous liquor had the highest antimicrobial activity. The total phenolic compounds content in ethylacetate, hexane, butanol, and aqueous fraction was 488.3 mg/g, 403.8 mg/g, 83.6mg/g, and 74.5 mg/g, respectively. Taken together, these results suggest that the ethyl acetate fraction could be suitable for the development of isolation and identification of antimicrobial compound from pyroligneous liquor, resulting from the above antimicrobial activity.

목초액의 천연보존료로서의 이용성을 검토하기 위하여 기계식 전용탄화로에서 제조된 목초액을 대상으로 항균활성과 총페놀성 화합물의 함량을 측정하였다.목초액을 농축하여 얻은 조추출물과 극성에 따라 분획한 용매분획물의 항균성을 측정한 결과 식중독균인 Salmonella enteritidis, Escherichia coli O157, Shigella sonnei, Yersinia enterocolitica, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes에 대하여 항균성을 나타내었다. 특히 hexane 분획과 ethyl acetate 분획이 다른 분획보다 항균성이 강하게 나타났으며, 균종별로는 Staphylococcus aureus, Listeria monocytogenes 등 그람 양성균이 그람 음성균에 비하여 항균활성이 높게 나타났다. 최소생육저해농도는 Listeria monocytogenes에 대해서는 두 분획 모두 125 μg/mL의 농도에서 생육을 억제하여 가장 높은 항균성을 보였고, Yersinia enterocolitica와 Staphylococcus aureus에 대하여 ethyl acetate 분획은 250 ${\mu}g/mL$ hexane 분획은 500 ${\mu}g/mL$의 농도에서 생육을 억제하였고 대부분의 다른 균에서도 hexane 분획과 ethyl acetate 분획은 500 ${\mu}g/mL$~1,000 ${\mu}g/mL$ 농도에서 생육을 억제하였다. 목초액의 ethyl acetate 분획을 silica gel column chromatography로 정제한 후 항균성을 시험한 결과 P-1과 P-2 fraction에서 강한 항균성 물질이 존재함을 확인하였다. 목초액의 조추출물과 용매분획물의 총페놀화합물의 함량을 측정한 결과 ethyl acetate 분획과 hexane 분획이 각각 488.3 mg/g와 403.8 mg/g으로 다른 분획에 비해 페놀성 물질의 함량이 높게 나타나 목초액의 항균성 물질은 페놀성 물질일 것으로 판단되었다.

Keywords

References

  1. Vargas, I., Sanz, I., Moya, P. and Prima-Yufera, E. (1999) Antimicrobial and antioxidant compounds in the nonvolatile fraction of expressed orange essence oil. J. Food Prot., 62, 929-932
  2. Himejima, M. and Kubo, I. (1991) Antimicrobial agents from the cashew Anacardium occidentate(Anacarrdiaceae) nut shell oil. J. Agric. Food Chem., 39, 418-421 https://doi.org/10.1021/jf00002a039
  3. Lee. H.S. and Ahn, Y.J. (1998) Growth-inhibition effects of Cinnamomum cassia bark-derived materials on human intestinal bacteria. J. Kor. Agric Chem. Soc., 46, 8-19 https://doi.org/10.1021/jf970548y
  4. Lee, B.W. and Shin, D.H. (1991) Screening of natural antimicrobial plant extract on food spoilage microo-rganisms. Kor. J. Food Sci. Technol., 23(2), 200-204
  5. Park, U.Y., Chang, D.S. and Cho, H.R. (1992) Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Nutr., 21(1), 91-96
  6. Smith, J.L. and Marmer, B.S. (1991) Growth temperature and action of lysozyme on Listeria monocytogenes. J. Food Sci., 56, 1101-1103 https://doi.org/10.1111/j.1365-2621.1991.tb14651.x
  7. Dickson, J.S. (1992) Acetic acid action on beef tissue surfaces contaminated with Salmonella typhimurium. J. Food Sci., 57, 297-301 https://doi.org/10.1111/j.1365-2621.1992.tb05480.x
  8. 渡邊昭宣 (1993) 米飯の腐敗および食中毒防止對策としての有機酸の效果. New Food Industry, 35(1), 65-78
  9. Delaquis, P.J., Sholberg, P.L. and Stanich, K. (1999) Disinfection of mung bean seed with gaseous acetic acid. J. Food Prot., 62, 953-957
  10. Wang, L.L. and Johnson, E.A. (1992) Inhibition of Listeria monocytogenes fatty acids and monoglycerides. Appl. Environ. Microbiol., 58(2), 624-629
  11. Smith-Palmer, A., Stewart, J. and Fype, L. (1998) Anti-microbial properties of plant essential oils and essences against five important food-borne pathogens. Lett. Appl. Microbio, 26, 118-122 https://doi.org/10.1046/j.1472-765X.1998.00303.x
  12. Oh, D.H., Ham, S.S., Park, B.K., Ahn, C. and Yu, J.Y. (1998) Antimicrobial of natural medicinal herbs on the food microorganism. Kor. J. Food Sci. Technol., 30, 957-963
  13. 전순자, 이계화, 설계신 (1998) 식물병충해 방제 및 사과 배 신선도 유지에 목초액의 이용. Res. Natural Resour., 1, 91-97
  14. 김영철, 이종원 (1993) 저농약 고품질 생산에 기여하는 목초액. 한국시설원예연구회 6(1), 105-119
  15. 永田耕一 (1984) 肝實質性黃疸における天然樹液の臨床的檢討. 基礎と臨床, 17(11), 1-19
  16. 川上純, 福島義信, 石橋撤夫 (1978) 特殊木醋液によう治療成績. 島根縣守, 醫學會發表要旨, 1-15
  17. 田村豊行 (1976) 藥物の解毒作用に關する硏究. 基礎と臨床, 9(13), 1-21
  18. Ikegami, F., Sekine, T. and Fujii, Y. (1998) Anti-dermaptophyte activity of phenolic compound imokusakueki. J. Pharmaceut. Soc. Japan, 118(1), 257-266
  19. 大槻 彰 (1997) 木炭, 木草液 健康法, 日本經濟通信社, 1997
  20. 서권일, 하기정, 배영일, 장진규, 심기환 (2000) 참나무 목초액의 항균효과. Kor. J. Postharvest Sci. Technol., 7(3), 337-341
  21. 유승헌 (1998) 유기농업에서 무공해 생물자원을 이용한 병충해 종합방제 기술개발-키토산, 식초, 목초액의 토마토 잎곰팡이병, 겹둥근무늬병, 오이흰가루병 억제효과와 토마토 뿌리혹선충 억제효과. 한국식물병리학회지, 14(3), 134-139
  22. Kim, J.S., Kim, J.C., Choi, J.S., Kim, T.J., Kim, S.M. and Cho, K.Y. (2001) Isolation and identification fo herbicidal substance from wood vinergars. Kor. J. Weed Sci., 21(4), 357-364
  23. Bauer, C., Kirby, W.M., Scheris, J.C. and Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Patho., 45, 493-499
  24. Kim, H.O., Park, S.W. and Park, H.D. (2004) Inactivation of Escherichia coli O157:H7 by cinnamic aldehyde purified from Cinnamomum cassia shoot. Food Microbiol., 21, 105-110 https://doi.org/10.1016/S0740-0020(03)00010-8
  25. A.O.A.C. Official Methods of Analysis, 13th ed., Association of Official Analytical Chemists, Washington. DC, USA (1980)
  26. Clark, A.M., EI-Feraly, F.S. and Li, W.S., (1981) Anti-microbial activity of phenolic constituent of Mangolina grandiflora L. J. Pharm. Sci., 70, 951-952 https://doi.org/10.1002/jps.2600700833