Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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High Throughput-compatible Screening of Anti-oxidative Substances by Insect Extract Library

약용곤충추출물 라이브러리를 이용한 항산화 활성의 초고속 검색

  • Park, Ja-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Heo, Jin-Chul (Department of Food Science and Technology, Kyungpook National University) ;
  • An, Sang-Mi (Department of Food Science and Technology, Kyungpook National University) ;
  • Yun, Eun-Young (AgroBiology Division, Rural Development Agency(RDA)) ;
  • Han, Sang-Mi (AgroBiology Division, Rural Development Agency(RDA)) ;
  • Hwang, Jae-Sam (AgroBiology Division, Rural Development Agency(RDA)) ;
  • Kang, Seok-Woo (AgroBiology Division, Rural Development Agency(RDA)) ;
  • Yun, Chi-Young (Department of Biology, Daejeon University) ;
  • Lee, Sang-Han (Department of Food Science and Technology, Kyungpook National University)
  • Published : 2005.10.01
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Abstract

Oxidant stress is well-known for a pivotal parameter related to neuro-inflammatory diseases including Alzheimer's disease, Parkinson's disease, and ALS (Lou Gehrig's disease). In order to effectively screen for anti-inflammatory agents, we first established the infrastructure of high throughput screening for anti-oxidant agents from medicinal insect library extracted with water, methanol, ethanol, and dimethyl sulfoxide. By the screening system, we found that Tenodera angustipennis Saussure, Pyrocoela rupa Olivier and Papilio maackii Mntris had strong anti-oxidant activity. Moreover, Tenodera angustipennis Saussure and Tenodera aridifolia (Stoll) exhibited protection effects of cellular damage by treatment of an oxidant hydrogen peroxide. Together, the results suggest that some selected hits could be a potential agent against neuro-inflammation, although the in vivo studies should be clearly tested.

산화적인 스트레스(Oxidative stress)는 신경염증의 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 약용곤충추출물을 이용하여 항산화 물질을 찾고자 초고속 적용가능한 스크리닝 방법을 적용하였다. 우선, 분자염증은 활성산소 관련의 물질과 밀접한 관련이 있으므로 이들의 억제를 동반하는 추출물을 먼저 선별하였다. 항산화 실험과 관련하여 DPPH (1,1-Diphenyl-2-picrylhydrazyl), FRAP(Ferric ion reducing antioxidant power), HO (Hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 assay하였고 hydrogen peroxide(H2O2)에 의한 세포사멸 억제 활성을 보기 위해 MTT assay를 실시하였다. 실험 결과, 사마귀, 늦반딧불이, 무당벌레에서 다른 library에 비교하여 항산화 활성이 높게 나타났다.

Keywords

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