Comparison of Pretreatment Methods for Extraction of Selected Components from Ganoderma lucidum

전처리 방법에 따른 영지버섯 추출액의 품질특성 변화

  • Chung, Hun-Sik (Food and Rio-Industry Institute, Kyungpook National University) ;
  • Youn, Kwang-Sup (Department of Food Science and Technology, Catholic University of Daegu)
  • 정헌식 (경북대학교 식품생물산업연구소) ;
  • 윤광섭 (대구가톨릭대학교 식품산업학부)
  • Published : 2005.04.01

Abstract

This study was conducted to investigate the effects of pretreatment and extraction methods on the water extraction yields of sugar, protein and phenolic compounds, and the antioxidative activity of extract from Ganoderma lucidum. The herb was ground, sifted, and treated for 10 min with microwave (2,450 MHz) and ultrasound (20 kHz), and then extracted by boiling underatmospheric pressure and pressured boiling. Particle size $(0.8\~2.4\;mm)$, extraction time (2 hr) and solvent/solid ratio (20:1) were selected as optimal conditions for extraction.. Microwave and ultrasound treatment increased the extraction yields of total sugar, protein and phenolic compounds and the radical scavenging activity of extract. In comparison with microwave treatment and ultrasound treatment, microwave treatment was more effective than that of ultrasound treatment. The yields of all three compounds and the radical scavenging activity of extract were higher in pressured boiling extraction than in boiling underatmospheric pressure. The results showed that microwave radiation and sonication prior to extraction, and pressured extraction could be utilized for improving the extraction efficiency of G. lucidum.

영지버섯(Ganoderma lucidum)으로부터 영양 및 기능성 성분의 열수추출 수율을 증대시킬 수 있는 방법을 개발하기 위하여 추출 전처리 및 방법의 효과를 비교, 검토하였다. 영지를 분쇄하여 사별하고 초음파와 마이크로파 처리를 10분 동안 실시한 후 물을 용매로 상압 및 가압추출을 각각 2시간 동안 실시하였다. 추출전 전처리를 행함으로서 총당, 단백질함량 및 페놀성물질의 추출수율을 증가시켰고, 유리기 소거능이 증가하는 경향을 보였으며 마이크로파 전처리가 초음파처리 보다 우수한 것으로 나타났다. 또한 상압추출보다 가압추출에 의해 가용성 고형물, 총당, 단백질 및 페놀성 물질의 추출수율이 증대되는 경향을 보였다. 따라서 초음파나 마이크로파 전처리와 가압추출은 영지버섯의 추출효율 향상에 유효한 방법인 것으로 판단되었다.

Keywords

References

  1. Kim, B.K. (1987) Pharmacological efficacy of G. lucidum. Korean J. Pharmacogn., 18, 58-60
  2. Shiao, M.S. (2003) Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. Chem. Rec., 3, 172-180. https://doi.org/10.1002/tcr.10058
  3. Kim, H.K, Kwon, Y.J., Kim, K.H. and Jeong, Y.H (2000) Changes of total polyphenol content and electron donating ability of aster glehni extracts with different microwaveassisted extraction conditions. Korean J. Food Sci. Technol., 32, 1022-1028
  4. Kwon, J.H, Lee, G.D., Kim, K.E., Belanger, J.M.R., and Pare J.R.J. (2004) Monitoring and optimization of microwave-assisted extraction for total solid, crude saponin, and ginsenosides from ginseng roots. Food Science and Biotechnology, 13, 309-3142
  5. Luque-Garcia, J.L. and Luque de Castro, M.D. (2004) Focused microwave-assisted soxhlet extraction: devices and applications. Talanta, 64, 571-577 https://doi.org/10.1016/j.talanta.2004.03.054
  6. Jo, Y.J., Lee, S.G., An, Y.H and Pi, J.H. (2003) Development of ultrasonication-assisted extraction process for manufacturing extracts with high content of pinosylvin from pine leaves. Agric. Biosys. Eng., 28, 325-334
  7. Park, J.H, Lee, H.S., Mun, H.C, Kim, D.H, Soong, N.S., lung, H.G., Bang, J.K and Lee,H.Y. (2004) Improvement of anticancer activation of ultrasonificated extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf, Rubus coreanus Miq. and Artemisia capillaris Thunb. Korean J. Medicinal Crop Sci., 12, 273-278
  8. Schinor, E.C., Salvador, MJ., Turatti, LC.C., Zucchi, O.L.A.D. and Dias, D.A. (2004) Comparison of classical and ultrasound-assisted extractions of steroids and triterpenoids from three Chresta spp. Ultrasonics Sonochemistry, 11, 415-421
  9. Lee, C.J., Kim, M.S., Shen, J.Y, Kim, YD. and Shin, J.H. (2003) The extraction condition of pungent compounds from Zanthoxylum piperltum D.C pericarps by using supereritical fluid extraction. Korean J. Medicinal Crop Sci., 11, 19-23
  10. Yang, S.J., Shin, J.S. and Kang, C,H.(2004) Extraction of acanthoside-D from Acanthopanax Cortex using supercritical carbon dioxide. Korean J. Biotechnol. Bioeng., 19, 284-287
  11. Brunner, G. (2005) Supercritical fluids: technology and application to food processing. J. Food Eng., 67, 21-33 https://doi.org/10.1016/j.jfoodeng.2004.05.060
  12. Dubois, M., Gilles, K.A., Hamilton, J.K, Revers, P.A. and Smith, F. (1956) Colorimetric method for determination of sugars and related substances. Anal. Chern., 28, 350-356 https://doi.org/10.1021/ac60111a017
  13. AOAC. (2000) Official Method of Analysis, 17th ed., Method 978.04, Association of Official Analytical Chemists, Arlington, VA, USA
  14. Lee, J.H. and Lee, S.R. (1994) Analysis of phenolic substances content in Korean plant foods. Korean J. Food Sci. Technol., 26, 310-316
  15. Blois, M.S. (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1204 https://doi.org/10.1038/1811199a0
  16. Toledo, R.T. (1999) Fundaments of food process engineering. 2th edn., Aspen Publication, MD, U.S.A., p.548-566
  17. Pan, X., Niu, G. and Liu, H. (2003) Microwave-assisted extraction of tea polyphenols and tea caffeine from green tea leaves, Chem. Eng. Process, 42, 129-133 https://doi.org/10.1016/S0255-2701(02)00037-5