Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Anticancer and Immuno-activity of Methanol Extract from Onion Kochujang

양파고추장 메탄올추출물의 항암 및 면역활성

  • Kim Jae-Yong (Research Institute of Basic Sciences, Sunchon National University) ;
  • Park Kyung-Wook (Department of Food Science and Nutrition, Sunchon National University) ;
  • Yang Hyun-Sook (Department of Food Science and Nutrition, Sunchon National University) ;
  • Cho Young-Sook (Department of Food Science and Nutrition, Sunchon National University) ;
  • Jeong Chang-Ho (Division of Applied Life Sciences, Graduate School, Gyeongsang National University) ;
  • Shim Ki-Hwan (Division of Applied Life Sciences, Graduate School, Gyeongsang National University) ;
  • Yee Sung-Tae (Department of Biology, Sunchon National University) ;
  • Seo Kwon-Il (Department of Food Science and Nutrition, Sunchon National University)
  • 김재용 (순천대학교 기초과학연구소) ;
  • 박경욱 (순천대학교 식품영양학과) ;
  • 양현숙 (순천대학교 식품영양학과) ;
  • 조영숙 (순천대학교 식품영양학과) ;
  • 정창호 (경상대학교 응용생명과학부) ;
  • 심기환 (경상대학교 응용생명과학부) ;
  • 이성태 (순천대학교 생물학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2005.04.01
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Abstract

Antitumor activities of methanol extract from onion Kochujang (MEOK) were investigated by using aflatoxin $B_{1}-mediated$ Salmonella typhimurium mutagenicity and the model of cytotoxicity on the cancer cell lines. Their immune activities were also investigated using mouse spleen cells and macrophage cell lines, respectively. MEOK showed the enhanced antimutagenicity in a dose-dependent manner. relative to the control group. MEOK decreased over $20\%$ of the proliferation of the A549(lung cancer cell) and MCF(breast cancer cell) cell lines at $1,000\;{\mu}g/mL$ concentration compared with the control cells. The proliferation of mouse spleen cells and the NO production in marcrophage cell lines treated MEOK were increased in a dose-dependent manner, respectively. The activities were higher than that of the control(no added onion) Kochujang.

양파고추장에 대한 항암 및 면역활성을 조사한 결과는 다음과 같다. 고추장 메탄을 추출물은 aflatoxin $B_{1}$으로 돌연변이를 유도한 Salmonella typhimurium에 대하여 농도 의존적으로 항돌연변이 효과가 큰 것으로 나타났으며, 양파를 첨가하지 않은 대조구 고추장보다 양파고추장이 그 효과가 더욱 크게 나타났다. 양파고추장 메탄올추출물은 A549 및 MCF-7 암세포주에 처리한 결과 대조구에 비하여 $1,000\;{\mu}g/mL$농도에서 모두 $20\%$ 이상 그 성장을 억제하였다. 양파 고추장 메탄올추출물은 농도에 비례하여 비장세포의 증식을 유도하였고, 대조구 고추장 추출물보다 더 높은 증식을 유도하였다. 고추장 추출물을 처리한 대식세포주에서 NO의 생성이 농도 의존적으로 증가하였으며, 역시 양파고추장 추출물이 대조구 고추장 추출물보다 더 많은 NO을 생성량을 유도하였다.

Keywords

References

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