Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Probiotic Properties of Lactic Acid Bacteria and Yeasts Isolated from Korean Traditional Food, Jeot-gal

젓갈로부터 분리된 젖산균 및 효모의 프로바이오틱 특성

  • Kim Seon-Jae (Department of Food Engineering and Food Industrial Technology Research Center, Mokpo National University) ;
  • Ma Seung-Jin (Department of Food Engineering and Food Industrial Technology Research Center, Mokpo National University) ;
  • Kim Hag-Lyeol (Solar Salt Biotechnology Research Center, Mokpo National University)
  • 김선재 (목포대학교 식품공학과 및 식품산업기술연구센터(RRC)) ;
  • 마승진 (목포대학교 식품공학과 및 식품산업기술연구센터(RRC)) ;
  • 김학렬 (천일염생명과학연구소)
  • Published : 2005.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to select probiotics having both a high survival rate and an ability to inhibit virulent pathogens, we have screened lactic acid bacteria and yeasts from Jeot-gal to examine their resistance to artificial gastric and bile juice. After being introduced in the artificial gastric acid for 2 hr, the isolated lactic acid bacteria and yeast were incubated for 24 hrs in the artificial bile juice. In particular, the strain ML 36, ML 128, and ML 178 survived the longest during 2 hr incubation period in the artificial gastric acid. All 3 strains of lactic acid bacteria, and 2 strains of yeast demonstrated higher growth rates than control in the artificial bile. In addition, the antimicrobial activity of lactic acid bacteria and yeasts was investigated to determine their efficiency as probiotic organisms. The lactic acid bacteria inhibited Gram positive and negative bacteria, while the yeast was marginally inhibited.

젓갈은 전남 목포지역의 대형마트 및 가정에서 수집하였으며 오징어젓, 갈치속젓, 꼴뚜기젓, 멸치젓 등 10여종의 젓갈로부터 균을 분리하였다. 이들 분리 유산균 중 3균주가 인공위액과 인공담즙액에서 내성을 나타내었다. 이들 분리된 균주 중 생존력이 왕성한 3균주는 모두 인공위액에서 2시간, 인공담즙액에서 24시간 생존 가능하였다. 특히 유산균 ML 36, ML 128, ML 178 외 2종의 효모는 초기 생균수와 인공위액에서 2시간 배양 후 생균수의 변화가 거의 없어 가장 강한 내성을 나타내었다. 인공위액에서 분리된 균주의 생존율은 낮은 pH로 인하여 낮은 경향을 나타내었으나, 인공담즙액에 대해서는 높은 생존율을 나타내었다. 인공위액 및 인공담즙에 내성을 나타낸 3종의 유산균이 항생물질인 nicin, rifamycin, streptomycin, 그리고 tetracycline에 대하여 내성을 나타내었다. 또한 이들 분리 유산균은 병원성 미생물인 Listeria monocytogenes 대해 뚜렷한 항균 효과가 있는 것으로 나타났다.

Keywords

References

  1. Lee, C.H., (1993) Fish fermentation technology, pp. 189-279. In Lee CH, Steinkraus KH. Reilly PJA (eds.). Fish Fermentation Technology in Korea, United Nations University Press, Tokyo, Japan
  2. Fernandez, M.F., Boris, S., and Barbes, C. (2003) Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract. J. Appl. Microbiol., 94, 445-449
  3. Lee, C.H., Jun, K.D., Kim, W.S., and Paik, H.D. (2001) Partial characterization of polyfennenticin SCD, a newly identified bacteriocin of Bacillus polyfennenticus. Lett. Appl. Microbiol., 32, 1-6
  4. Osullivan, G.C. (2001) Probiotics. Brit. J. Surg., 88, 161-162 https://doi.org/10.1046/j.1365-2168.2001.01656.x
  5. Ahn, Y.T., Kim, Y.H., Jung, E.J., Lim, H.T., Kang, H.J., and Kim, H.U. (1999) Resistance of lactobacilli and bifidobacteria isolated from fermented milk products to low pH and bile acid. Kor. J. Anim. Sci., 41, 335-342
  6. Cho, MK, Kim, K, Kim, C.H., Lee, T.K, and Kim, K.Y. (2000) Isolation and characterization of Lactobacillus fermentum YL-3 as a poultry probiotic. Kor. J. Appl. Microbiol. Biotechnol., 28, 279-284
  7. Lee, S.H, and No, M.J. (1997) Viability in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi. Kor. J. Appl. Microbiol. Biotechnol., 25, 617-622
  8. Xanthopoulos, V., Litopoulou-Tzanetaki, E., Tzanctakis, N. (2000) Characterization of Lactobacillus isolates from infant feces as dietary adjuncts. Food Microbiol., 17, 205-215 https://doi.org/10.1006/fmic.1999.0300
  9. Tannock, G.W. (1997) Probiotic properties of lactic acid bacteria: plenty of scope for fundamental R&D. Trends. Biotechnol., 15, 270-274 https://doi.org/10.1016/S0167-7799(97)01056-1
  10. Fuller, R (1989) Probiotics in man and animals. J. Appl. Bacteriol., 66, 365-378 https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  11. Goldin, B.R, and Borbach, S.L. (1977) Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements, and dimethylhydrazine. Cancer, 40, 2421-2426 https://doi.org/10.1002/1097-0142(197711)40:5+<2421::AID-CNCR2820400905>3.0.CO;2-I
  12. Gilliland, S.E., and Walker, DK(1990) Factors to consider when selecting a culture of Lactobacillus acidophilus as an dietary adjunct to produce an hypocholesterolemic effect in humans. J. Dairy Sci., 73, 901-911
  13. Fuller, R (1991) Probiotics in human medicine. Gut, 32, 439-442 https://doi.org/10.1136/gut.32.4.439
  14. Hilton, E., Henry, D.I., Phyllis, A, Kenneth, F. and Michael, T. B. (1992) Ingestion of yoghurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann. Int. Med., 116, 353-357 https://doi.org/10.7326/0003-4819-116-5-353
  15. Shih, N. (1994) Lactobacillus acidophilus and lactose intolerance, a review. ASEAN Food J., 9, 47-54
  16. Matsuzaki, T., Hashimoto, S., and Yokokura, T. (1996) Effects on antitumor activity and cytokine production in the thoracic cavity by intrapleural administration of Lactobacillus casei in tumor-bearing mice. Med. Microbiol. Immunol. Berl., 185, 157-161 https://doi.org/10.1007/s004300050026
  17. Takiguchi, R, Mochizuki, E., Suzuki, Y, Nakajima, I., and Benno, Y (1997) Lactobacillus acidophilus SBT 2062 and Bifidobacterium longum SBT 2928 on harmful intestinal bacteria. J. Int. Microbiol., 11, 11-17
  18. Jun, K.D., Lee, K.H., Kim, W.S., and Paik, H.D. (2000) Microbiological identification of medical probiotic bispan strain. Kor. J. Appl. Microbiol. Biotechnol., 28, 124-127
  19. Paik, H.D., Jun, K.D., Sung, S.H, Kim, W.S., Kim, HS., and Lee, B.c. (1999) Liquid cultivation of strains of Bacillus polyjermenticus. United States Patent, 6,010,898
  20. Kobayashi, Y., Tohyama, K, and Terashima, T (1974) Tolerance of multiple antibiotic resistant strain, L. casei PPSR 3002, to artificial digestive fluids. Jpn. J. Microbiol., 29, 691-697
  21. Lee, N.K., Kim, HW., Cho, S.Y., and Paik, H.D. (2003) Some probiotic properties of some lactic acid bacteria yeasts isolated from leot-gal. Kor. J. Microbiol. Biotechnol., 31, 297-300
  22. Paik, H.D., Jung, M.Y., Jung, H.Y., Kim, W.S. and Kim, K.T (2002) Characterization of Bacillus polyfermenticus sen for oral bacteriotherapy of gastrointestinal disorders. Korean J. Food Sci. Technol., 34, 73-78
  23. Chung, S.H., Suh, H.J., and Lee, H. (1997) Utilization of soybean curd whey as a medium for Lactobacillus acidophilus and acid-and bile-tolerance of cultured strains. J. Korean Soc. Food Sci. Nutr., 26, 872-877
  24. Hong, S.I., Kim, Y.J., and Pyun, Y.R. (1999) Acid tolerance of Lactobacillus plantarum from Kimchi. Lebensm. -Wiss. U-Technol, 32, 142-148 https://doi.org/10.1006/fstl.1998.0517
  25. Lee, S.H., and No, M.J., (1997) Viability in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi. J. Appl. Microbiol. Biotechnol., 6, 617-622
  26. Conway, P.L., Gorbach, S.L., and Goldin, B.R. (1987) Survival of lactic acid bacteria in human stomarch and adhesion to intestinal cells. J. Dairy Sci., 70, 1-12 https://doi.org/10.3168/jds.S0022-0302(87)79974-3
  27. Giannella, R.A., Broitman, S.A, and Zamcheck, N. (1972) Gastric barrier to ingested microorganil-. in man : Studies in vivo and in vitro. Gut, 13, 251-256 https://doi.org/10.1136/gut.13.4.251
  28. Berrada, N., Lemeland, J.F., Laroche, G., Thouvenot, P., and Piaia, M. (1991) Bifidobacterium from fermented milks: Survival during gastric transit. J. Dairy Sci., 74, 409-413 https://doi.org/10.3168/jds.S0022-0302(91)78183-6
  29. Kim, G.B., Lee, J.H., Lim, K.S., Huh, C.S., Bae, H.S., Baek, Y.J. and Kim, H.U.,(1999) Bile salt deconjugation activity of Lactobacillus strains isolated from yogurt products. Kor. J. Appl. Microbiol. Biotechnol., 27, 385-390
  30. Gilliand, S.E., Staley, T.E. and Bish, L.J. (1984) Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci., 67, 3045-3051 https://doi.org/10.3168/jds.S0022-0302(84)81670-7
  31. Shin, Y.S., Kim, S.H., and Lee, K.S. (1995) Survivals of lactic acid bacteria and its characteristics under the acidic and anaerobic condition. Kor. J. Appl. Microbiol. Biotechnol., 23, 373-377