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부산지역에서 분리한 레지오넬라균에 대한 PFGE를 이용한 molecular typing

Molecular Typing of Legionella pneumophila Isolated in Busan, Using PFGE

  • 박은희 (부산광역시 보건환경연구원) ;
  • 김미희 (부산광역시 보건환경연구원) ;
  • 김정아 (부산광역시 보건환경연구원) ;
  • 한난숙 (부산광역시 보건환경연구원) ;
  • 이주현 (부산광역시 보건환경연구원) ;
  • 민상기 (부산광역시 보건환경연구원) ;
  • 박연경 (부산광역시 보건환경연구원) ;
  • 진성현 (부산광역시 보건환경연구원) ;
  • 정구영 (부산광역시 보건환경연구원) ;
  • 빈재훈 (부산광역시 보건환경연구원)
  • Park Eun-Hee (Busan Metropolitan City Institute of Health & Environment) ;
  • Kim Mi-Hee (Busan Metropolitan City Institute of Health & Environment) ;
  • Kim Joung-A (Busan Metropolitan City Institute of Health & Environment) ;
  • Han Nan-Sook (Busan Metropolitan City Institute of Health & Environment) ;
  • Lee Ju Hyeoun (Busan Metropolitan City Institute of Health & Environment) ;
  • Min Sang Gi (Busan Metropolitan City Institute of Health & Environment) ;
  • Park Yon Koung (Busan Metropolitan City Institute of Health & Environment) ;
  • Jin Seong Hyun (Busan Metropolitan City Institute of Health & Environment) ;
  • Jeong Gu Young (Busan Metropolitan City Institute of Health & Environment) ;
  • Bin Jae Hun (Busan Metropolitan City Institute of Health & Environment)
  • 발행 : 2005.04.01

초록

2001년부터 2003년까지 부산지역의 냉각탑수에서 분리한 L. pneumophila (serogroup 1) 39균주에 대해 제한효소 SfiI 처리한 경우 PFGE 양상은 dice coefficient $<65\%$의 유사성을 가진 band를 Awl로 10개의 pulsotype으로 나누었고, 가장 많았던 유형은 E pulsotype으로 39주중 18주로 $46.2\%$를 나타내었으며, 그 외 A pulsotype $17.9\%$, C pulsotype $15.4\%$, F pulsotype $7.7\%$ 및 B, D, G, H, I, J pulsotype이 각각 $2.6\%$로 유전적 양상이 다양하였다. 제한효소 NotI 처리한 경우 PFGE 양상은 dice coefficient $<60\%$의 유사성을 가진 band를 $a\~h$로 8개의 pulsotype으로 나누었고, 가장 많았던 유형은 f pulsotype이 $38.5\%$였으며 그 외 d pulsotype $20.5\%$, e pulsotype $17.9\%$, a pulsotype $10.3\%$, h pulsotype $7.7\%$ 및 b, c, g pulsotype이 각각 $2.6\%$를 차지하였다. 본 연구를 통하여 부산지역에서 분리되는 레지오넬라균에 대한 유전자 유형분석 결과를 데이터베이스화하여, 레지오넬라증의 집단 발생 또는 산발적인 질병 발생이 있을 경우 분자학적 측면에서 사람과 환경에서 분리된 균으로부터 감염원과 감염경로를 규명하는 역학적인 도구로써 이용 가능할 것으로 사료되었다.

In this study, we did the molecular typing of 39 environmental Legionella pneumophila serogroup 1 isolates collected from 2001-2003 in Busan using the pulsed-filed gel electrophoresis (PFGE). PFGE of SfiI fragments were divided into 10 pulsotypes $(A\~J)$, corresponding to $<65\%$ similarity and a subtype within each pulsotype was characterized by $>84\%$ similarity. The major cluster was pulsotype E $(46.2\%)$, which included 18 isolates and was divided into 4 subtypes $(E1\~E4)$. PFGE of NotI fragments were divided into 8 pulsotypes $(a\~h)$, corresponding to $<60\%$ similarity and a subtype within each pulsotype was characterized by $100\%$ similarity. The major cluster was pulsotype f $(38.5\%)$, which included 15 isolates. The ATCC type strain L. pneumophila serogroup 1 was identified as a different molecular pulsotype compare to the Busan isolates. It is possible that L. pneumophila serogroup 1 isolated in Busan with specific DNA pattern is comparable with those isolation in other cities in Korea.

키워드

참고문헌

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  1. Molecular Epidemiology ofLegionella pneumophilaIsolated from Bath Facilities of Public Establishments in Seoul vol.41, pp.4, 2011, https://doi.org/10.4167/jbv.2011.41.4.295
  2. Molecular Genetic Typing ofLegionella pneumophilaStrains Isolated in Jejudo, Korea vol.40, pp.3, 2010, https://doi.org/10.4167/jbv.2010.40.3.111
  3. Molecular Epidemiology of Legionella pneumophila Isolated from Water Supply Systems in Seoul, Korea vol.39, pp.2, 2013, https://doi.org/10.5668/JEHS.2013.39.2.166