Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Studies on the Freezing of Boar Semen I. Effects of Cooling Rate and Extenders on Viability and Normal Acrosome after Frozen-Thawed of Boar Semen

돼지정액의 동결에 관한 연구 I. 냉각속도와 희석액이 동결융해후 정자의 생존성과 정상첨체율에 미치는 영향

  • Published : 2005.04.01
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Abstract

This experiment was carried out to investigate the extender, cooling rate and concentration of glycerol for freezing of boar semen. The result obtained were summarized as follows: 1. Optimal cooling rate was $0.17\~022^{\circ}C/min$ from 25 to $5^{\circ}C$ in LEY extender on the viability and normal acrosome after thawed. 2. The LEY extender was effective in protecting frozen boar semen from cold shock among the extenders(p<0.001, respectively). 3. The sperm viability and normal acrosome rates after thawing was showed greater in the 3 or $4\%$ of glycerol concentration than $2\%$ in LEY extender. 4. Viability of sperm was higher when both 15mM of fructose and 3 or $4\%$ glycerol were added to the LEY extender compared with other concentrations of fructose and glycerol were added it(p<0.001).

본 실험은 돼지정액의 동결보존을 위한 희석액과 냉각속도 및 동해방지제의 적정농도를 결정하기 위해 실시하였으며, 얻어진 결과는 다음과 같다. 1. $25^{\circ}C$에서 $5^{\circ}C$까지의 냉각속도에서는 LEY 희석액에서 분당 $0.17\~0.22^{\circ}C$로 냉각하는 것이 생존율과 정상 첨체율에서 가장 높은 결과를 얻었다. 2. LEY 희석액이 BF5와 M-Soejima 희석액보다 정자를 동해로부터 보호하는 능력이 우수하였다. 3. LEY 희석액에 첨가하는 glycerol의 농도는 3 또는 $4\%$가 $2\%$의 glycerol을 첨가한 것보다 4. LEY 희석액에 15mM의 fructose와 $3\%$ 또는 $4\%$의 glycerol 첨가구가 가장 높은 생존율과 정상첨체율을 나나냈다(p<0.001).

Keywords

References

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