유산균이 Aspergillus parasiticus의 성장과 Aflatoxin $B_1$ 생성에 미치는 영향

The Effects of Lactic Acid Bacteria on the Growth and Aflatoxin $B_1$ Production of Aspergillus parasiticus

  • 이경민 (계명대학교 대학원 공중보건학과) ;
  • 김종규 (계명대학교 대학원 공중보건학과)
  • Lee Kyung-Min (Department of Public Health, Graduate School, Keimyung University) ;
  • Kim Jong-Gyu (Department of Public Health, Graduate School, Keimyung University)
  • 발행 : 2005.04.01

초록

This study was performed to investigate the competitive effect of Leuconostoc mesenteroides and Lactobacillus plantarum on the growth and aflatoxin $B_1$ production of Aspergillus parasiticus. Leu. mesenteroides and Lac. plantarum were grown with A. parasiticus in a modified APT broth at $28^{\circ}C$ for 9 days. It was observed that the growth of A. parasiticus got lower when the two bacteria were inoculated simultaneously than when the mold grew alone. The pH of mixed culture of Leu. mesenteroides and Lac. plantarum showed significantly lower value than the pH of the pure culture of A. parasiticus (p<0.05). The acidity of the mixed culture group significantly increased compared with the control group (A. parasiticus alone group) (p<0.05). The aflatoxin $B_1$ production was significantly decreased in the mixed culture group than in the A. parasiticus alone group (p<0.05). Leu. mesenteroides showed the more efficient effect than Lac. plantarum. These results indicate that the two lactic acid bacteria have inhibitory effect on the growth and aflatoxin $B_1$ production of Aspergillus parasiticus.

키워드

참고문헌

  1. Smith, J. E. and Moss, M. O. : Mycotoxins, formation, analysis and significance. John Wiley & Sons, Chichester, 3-103, 1985
  2. 이용욱,정영채,신광순,신효선 : 최신식품위생학,114-117,1989
  3. Newbern, P. M. and Rogers, A. E. : J. National Cancer. Institute, 50, 439, 1973
  4. Grobstein, C. : Diet, Nutrition and Cancer: National Academy Press, 401-403, 1982
  5. 이태령,이상규: 식품 중 유독성 대사산물에 관하여 (제1보) -수종의 한국대두 발효식품 중의 aflatoxin 유무의 검색에 대하여 한국식품과학회지,1(1),78-84, 1969
  6. 이용욱,김종규 : 한국산 일부 곡류에 대한 aflatoxin $B_{1}$, 조사연구. 국민보건연구소연구논총, 1(1),117-122,1991
  7. 손동화 : ELISA에의한 농산물 중 aflatoxin 잔류조사. 한국식품위생학회지,12(4),192-193, 1997
  8. 윤미혜. 수입식품 및 국내식품 중의 aflatoxin 함유량 조사연구. 대한위생학회지, 13(2),9-88,1998
  9. 김종규: 자외선 조사에 의한 Aflatoxin 감소 효과-고체 배지를 중심으로. 대한보건협회학술지,26(4),497-502,2000
  10. Wei, R. D. and Chu, F. S. : Aflatoxin-solvent interactions induced by ultraviolet light. J. Assoc. Office. Anal. Chem., 56, 1425-1430, 1973
  11. Altug, T., Yousef, A. E. and E. H. : Degradation of aflatoxin B, in dried figs by sodium bisulfate with or without heat, ultraviolet energy or hydrogen peroxide. J. Food Prot, 53(7), 581-582, 1990 https://doi.org/10.4315/0362-028X-53.7.581
  12. Icibal, N. and Altug, T. : Degradation of aflatoxin in dried figs by sulphur dioxide alone and in combination with heat, ultraviolet energy and hydrogen peroxide. Lebensrn. Wiss. U. Tech., 25, 294-296, 1992
  13. 김종규 : 실험실 폐수 중 aflatoxin 감소를 위한 화학적 처리에 관한 연구. 한국환경위생학회지,18(2),52-56,1992
  14. 여현종,김종규: 화학적 처리가 옥수수의 aflatoxin 감소에 미치는 영향. 한국환경위생학회지,29(5),126-132, 2003
  15. Cucullin, A. F. : Ammoniation of aflatoxin $B_{1}$ J. Agr. Food Chem, 24, 408-414, 1976 https://doi.org/10.1021/jf60204a011
  16. Maerck, K. E. : Aflatoxin destruction in com using sodium bisulfite, sodium hydroxide and aqueous ammonia. J. Food. Prot, 43, 571-574, 1980 https://doi.org/10.4315/0362-028X-43.7.571
  17. Hagler, W. M. Jr. : Destruction of aflatoxin in com with sodium bisulfite. J. Food Prot., 45, 1287-1291, 1982 https://doi.org/10.4315/0362-028X-45.14.1287
  18. Hurst, A. : Biosynthesis of the antibiotic nisin by whole Streptococcus lactis organisms. J. Gen. Microbioi, 44, 209-220, 1996
  19. Wiseman, D. W. and March, E. H. : Growth and aflatoxin production by Aspergillus parasiticus when in the presence of Streptococcus lactis. Mycopathologia, 73, 49-56, 1981 https://doi.org/10.1007/BF00443014
  20. Karunataten, A., Wenxenberg, E. and Bullerman, L. B. : Inhibition of mold growth and aflatoxin production by Lactobacillus spp. J. Food Prot., 53, 230-236, 1990 https://doi.org/10.4315/0362-028X-53.3.230
  21. Weckbach, L. S. and Marth, E. H. : Aflatoxin production by Aspergillus parasiticus in a competitive environment. Mycopathologia, 62, 39-45, 1977 https://doi.org/10.1007/BF00491994
  22. El-Gendy, S. M. and Marth, E. H. : Growth and aflatoxin production by Aspergillus parasiticus in the presence of Lactobacillus casei. J. Food Prot., 44(3), 211-212, 1981 https://doi.org/10.4315/0362-028X-44.3.211
  23. 김종규, 강희양,민경진 : HPLC에 의한 aflatoxin 분석 법에 관한 연구 - 형광 및 자외선 흡광 검출의 비교. 한국환경위생학회지,22(1),36-44, 1996
  24. 신동균,이용욱,김종규,정덕화 : Peniciilium citrinum 의 생성과 citrinin 생성에 미치는 젖산균의 영향에 관한 연구. 한국식품위생학회지,6(3),119-126,1991
  25. Kim, J. G., Lee, Y. W., Kim, P. G., Roh, W. S. and Hideharu Shintani : Reduction of aflatoxins by Korean soybean paste and its effect on cytotoxicity and reproductive toxicity - Part 1. J. Food Prot., 63(9), 1295-1298, 2000
  26. 강길진,정지현,조정일 : Bacillus subtilis가 생산하는 길항물질에 의한 아플라톡신 생성균 억제. 한국식품 위생안전성학회지,15(2),122-127,2000
  27. 김종규. 윤준식: 김치의 숙성 및 발효중 오염지표미생물과 유산균의 변화- 제1보. 한국환경위생학회지,31(1),79-85,2005
  28. Coallier-Ascah, J. and Idziak, E. E. : Interaction between Streptococcus lactis and Aspergillus flavus on production of aflatoxin. Appl. Env. Microbiol, 49, 163-167, 1988
  29. 박인숙: 김치 유산균의 생리적 특성과 기능 중앙대학교 박사학위논문,1992
  30. 이창호,박희동:김치로부터 항돌연변이 물질을 생산하는 유산균의 분리 및 특성. 한국산업미생물학회지,27(1),15-21, 1999