Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The quantitative analysis by digital subtraction radiography on the effect of Enamel Matrix Protein and Platelet-Rich Plasma, combined with Xenograft in the treatment of intrabony defect in humans

골 내 결손 치료 시 법랑 기질 단백질과 이종골 이식 및 혈소판 농축 혈장의 골 재생 효과에 대한 디지털 공제술의 정량적 분석

  • Han, Keum-Ah (Department of Periodontology, College of Dentistry, Dan-Kook University) ;
  • Lim, Sung-Bin (Department of Periodontology, College of Dentistry, Dan-Kook University) ;
  • Chung, Chin-Hyung (Department of Periodontology, College of Dentistry, Dan-Kook University) ;
  • Hong, Ki-Seok (Department of Periodontology, College of Dentistry, Dan-Kook University)
  • 한금아 (단국대학교 치과대학 치주과학교실) ;
  • 임성빈 (단국대학교 치과대학 치주과학교실) ;
  • 정진형 (단국대학교 치과대학 치주과학교실) ;
  • 홍기석 (단국대학교 치과대학 치주과학교실)
  • Published : 2005.12.31
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Abstract

Various biological approaches to the promotion of periodontal regeneration have been used. These can be divided into the use of growth and differentiation factors, application of extracellular matrix proteins and attachment factors and use of mediators of bone metabolism. The purpose of this study was to evaluate the effect of enamel matrix protein and platelet-rich plasma on the treatment of intrabony defect, with bovine-derived bone powder in humans by digital subtraction radiography. 12 teeth(experimental I group) were treated with enamel matrix protein combined with bovine-derived bone powder and 12 teeth(experimental II group) were treated with platelet-rich plasma combined with bovine-derived bone powder. The change of bone density was assessed by digital subtraction radiography in this study. The change of mineral content was assessed in the method that two radiography were put into computer program to be overlapped and the previous image was subtracted by the later one. Both groups were statistically analyzed by Wilcoxon signed Ranks Test and Mann-whitney Test using SPSS program for windows(5% significance level). The results were as follows: 1. The radiolucency in 3 months after surgery was significantly increased than 1 month after surgery in both groups(experimental I and II groups)(p<0.05). 2. The radiopacity in 6 months after surgery was significantly increased than 3 months after surgery in both groups(experimental I and II groups) (p<0.05). 3. In experimental I group, there was no significant difference between 1 month and 6 months after surgery. 4. In experimental II group. the radiopacity in 6 months after surgery was significantly increased than 1 month after surgery(p<0.05). 5. There was no significant difference between experimental I and II group at 1 month and 3 months after surgery, but the radiopacity in experimental II group was significantly increased at 6 months after surgery(p<0.05). In conclusion, platelet-rich plasma can enhance bone density than enamel matrix protein until 6 months after surgery.

Keywords

References

  1. Hideaki Hirooka. The biologic concept for the use of enamel matrix protein : True periodontal regeneration. Quintessence international 1998:29:621-630
  2. Cochran DL., King GN., Schoolfield J., Velasquez-Plata D., Mellonig JT., Jones A. The effect of enamel matrix proteins on periodontal regeneration as determined by histological analyses. J Periodontol 2003: 74: 1043-55 https://doi.org/10.1902/jop.2003.74.7.1043
  3. American Academy of Periodontology. Glossary of Periodontal Terms. ed 3. Chicago : American Academy of Periodontology, 1992:46
  4. Cochran DL., Wozney JM. Biological mediators for periodontal regeneration. Periodontology 2000 1999: 19:40-58 https://doi.org/10.1111/j.1600-0757.1999.tb00146.x
  5. Hoang AM., Oates TW., Cochran DL. In vitro wound healing responses to enamel matrix derivative. J Periodontol 2000 Aug:71:1270-7 https://doi.org/10.1902/jop.2000.71.8.1270
  6. Yukna RA., Mellonig JT. Histologic evaluation of periodontal healing in humans following regenerative therapy with enamel matrix derivative. A 10-case series. J Periodontol. 2000 May: 71: 752-9 https://doi.org/10.1902/jop.2000.71.5.752
  7. Marx RE., Carlson ER., Eichstaedt RH., Schimmele SR.. Strauss JE.. Georgeff KR. Platelet-rich plasma. Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol 1998:85:638-646 https://doi.org/10.1016/S1079-2104(98)90029-4
  8. Canalis E.. Mc Carthy TL.. Centrella M. The role of growth factors in skeletal remodelling. Endocrimol Meta Clin North Am 1989:18:903-912
  9. Antoniades HN.. Scher CD., Stiles CD. Purification of human platelet-derived growth factor. Cell Biol 1979: 76: 1809-1813
  10. Lynch SE., Williams RC., Polson AM., Howell TH., Reddy Ms., Zappa UE .. Antoniades HN. A combination of platelet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 1989:16:545-548 https://doi.org/10.1111/j.1600-051X.1989.tb02334.x
  11. Hoang AM., Klebe RJ., Steffensen B., Ryu OH., Simmen JP., Cochran DL. Amelogenin is a cell adhesion protein. J Dent Res 2002: 81 : 497-500 https://doi.org/10.1177/154405910208100713
  12. Nyman S., Gottlow J., Karring T., Lindhe J. The regenerative potential of the periodontal ligament. An experimental study in the monkey. J Clin Periodontol 1982; 9: 257 https://doi.org/10.1111/j.1600-051X.1982.tb02065.x
  13. Melcher AH., McCulloch CAG., Cheong T., Nemeth E., Shiga A. Cells from bone synthesize cementum-like and bone-like tissue in vitro and may migrate into periodontal ligament in vivo. J Periodontal Res 1987:22:246
  14. Melcher AH. On the repair potential of periodontal tissues. J Periodontol 1976: 47:256 https://doi.org/10.1902/jop.1976.47.5.256
  15. Gottlow J.. Nyman S., Lindhe J., Karring T.. Wennstrom J. New attachment formation in the human periodontium by guided tissue regeneration. Case reports. J Clin Periodontol 1986; 13: 604 https://doi.org/10.1111/j.1600-051X.1986.tb00854.x
  16. Terranova VP.. Hick S., Franzetti L.. Lyall RM.. Wikesso VME. A biochemical approach to periodontaol regeneration. AFS CM. Assay for specific cell migration. J Periodontol 1987;58:247-259 https://doi.org/10.1902/jop.1987.58.4.247
  17. Terranova VP.. Franzetti LC., Hick S.. Wikesjo VME. Biochemically mediated periodontal regeneration. J Periodont Res 1987;22:248-251 https://doi.org/10.1111/j.1600-0765.1987.tb01580.x
  18. Rutherford RB.. Trilsmith MD.. Ryan HE.. Charette MF. Synergistic effects of dexamethasone on platelet-derived growth factors mitogenesis in vitro. Arch Oral Biol 1992;37:139-145 https://doi.org/10.1016/0003-9969(92)90009-W
  19. Matsuda N., Lin WL., Kumar NM., Cho MI., Genco RJ. Mitogenic chemotactic and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro. J Periodontol 1992; 63: 515-525 https://doi.org/10.1902/jop.1992.63.6.515
  20. Oates TW., Rouse CA., Cochra DI. Mitogenic effects of growth factors on human periodontal ligament cells in vitro. J Periodontol 1993 ;64: 142-148 https://doi.org/10.1902/jop.1993.64.2.142
  21. Giannobile WV., Finkelman RD., Lynch SE. Comparison of canine and nonhuman primate animal models for periodontal regenerative therapy: Results following a single administration of PDGF/IGF-I. J Periodontol 1994;65: 1158-1168 https://doi.org/10.1902/jop.1994.65.12.1158
  22. Sporn MB.. Roberts AB., Wakefield LM. , de Crombrugghe B. Some recent advances in the chemistry and biology of TGF-$\beta$. J Cell Biol 1987:105:1039-1045 https://doi.org/10.1083/jcb.105.3.1039
  23. Keski-Oja J.. Leof EB.. Lyons RM.. Coffey RJ.. Jr. Moses HL. Transforming growth factor and control of neoplastic cell growth. J Cell Biochem 1987: 33: 95-107 https://doi.org/10.1002/jcb.240330204
  24. Antosz ME.. Bellows CG.. Aubin JE. Effects of TGF-$\beta$ and epidermal growth factor on celll proliferation and the formation of bone nodules in isolated fetal rat calvaria cells. J Cell Physiol 1989: 140:386-393 https://doi.org/10.1002/jcp.1041400225
  25. Centrella M.. McCarthy TL., Canalis E. TGF-$\beta$ is a bifunctional regulator of recplication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone. J Bio Chem 1987:262: 2874-2896
  26. Subburaman Mohan. Bone growth Factors. Clinical Orthopaedics and related research 1991:263:30-48
  27. Lynch SE., Williams RC., Polson AM., Howell TH., Reddy Ms., Zappa UE., Antoniades HN. A combination of platelet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 1989: 16:545-548 https://doi.org/10.1111/j.1600-051X.1989.tb02334.x
  28. Lynch SE. Platelet-derived growth factor and insulin-like growth factor. I. Mediators of healing in soft tissue and bone wounds. Periodont Case Rep 1991: 13: 13-20
  29. Thorarinn J. Sigrudsson, Lauralee Nygaard. Periodontal repair in dogs : Evaluation of rh BMP-2 carriers. Int J Periodont Res Dent 1996: 16: 525-537
  30. Clergeau LP., Danan M., Clergeau-Guerithault S., Brion M. Healing response to anorganic bone implantation in periodontal intrabony defects in dogs. Part 1. Bone regeneration. A microradiographic study. J Periodontol. 1996 Feb:67: 140-9 https://doi.org/10.1902/jop.1996.67.2.140
  31. Grondahl H., Grondahl K. Subtraction radiography for the diagnosis of periodontal bone lesions. Oral Surg 1983;55: 208. https://doi.org/10.1016/0030-4220(83)90180-9
  32. Grondahl H., Grondahl K., Webber RL. A digital subtraction technique for dental radiography. Oral Surg 1983:55 :96. https://doi.org/10.1016/0030-4220(83)90314-6
  33. Gröndahl H., Gröndahl K., Webber RL. A digital subtraction technique for dental radiography. Oral Surg Oral Med Oral Pathol 1988;65:490-494 https://doi.org/10.1016/0030-4220(88)90365-9
  34. Ortman LF., Dunford R., McHenry K., Hausmann E. Subtaction radiography and computer assisted densitometric analyses of standardized radiographs. J of Periodontol 1985: 20: 644-651 https://doi.org/10.1111/j.1600-0765.1985.tb00849.x
  35. Dunn SM., van der Stelt AF., Ponce A., Feeney K., Shah S. A comparison of two registration technique for digital subtaction radiography. Dentomaxillofac Radiol 1993;22: 77-80 https://doi.org/10.1259/dmfr.22.2.8375559
  36. Souyris F., et al. Coral. a new biomedicals materials. J. Max-fac. Surg 1985; 13:64
  37. Ericson K., Söderman M., Maurincomme E., Lindquist C. Clinical experience with stereotactic digital subtraction angiography with distortion correction software. Sterotact Funct Neurosurg 1996: 66:63-70. https://doi.org/10.1159/000099771
  38. 김은경. 디지털 공제술에서 비표준화 방사선사진의 대조도 및 기하학적 보정에 관한 연구. 대한치주과학회지 1998:28:797-808
  39. Canalis E., Mc Carthy TL., Centrella M. The role of growth factors in skeletal remodelling. Endocrimol Meta Clin North Am 1989:18:903-912
  40. Antoniades HN., Scher CD., Stiles CD. Purification of human platelet-derived growth factor. Cell Biol 1979:76: 1809-1813
  41. Brooks DB., Heiple KG., et al. Immunological factors in homogeneous bone transplantation. N. The effect of various methods of preparation and irradiation on antigenicity. J bone Joint Surg 1963: 45:1617 https://doi.org/10.2106/00004623-196345080-00004
  42. Ortman LF., Dunford R., McHenry K., Hausmann E. Subtraction radiography and computer assisted densitometric analyses of standardized radiographs. J of Periodontol 1985:20:644-651 https://doi.org/10.1111/j.1600-0765.1985.tb00849.x
  43. Dunn SM., van der Stelt AF., Ponce A., Feeney K., Shah S. A comparison of two registration technique for digital subtraction radiography. Dentomaxillofac Radiol 1993:22:77-80 https://doi.org/10.1259/dmfr.22.2.8375559
  44. Ericson K., Soderman M., Maurincomme E., Lindquist C. Clinical experience with stereotactic digital subtraction angiography with distortion correction software. Sterotact Funct Neurosurg 1996: 66:63-70 https://doi.org/10.1159/000099771
  45. Sculean A., Donos N., Windisch P., Brecx M., Cera I.. Reich E., Karring T. Healing of human intrabony defects following treatment with enamel matrix proteins or guided tissue regeneration. J Periodontal Res 1999:34:310-22 https://doi.org/10.1111/j.1600-0765.1999.tb02259.x
  46. Venezia E., Goldstein M., Schwartz Z. The use of enamel matrix derivative in periodontal therapy. Refuat Hapeh Vehashinayim 2002; 19: 19-34.88
  47. Sculean A.. Chiantella GC., Windisch P., Gera I.. Reich E. Clinical evaluation of an enamel matrix protein derivative (Emdogain) combined with a bovinederived xenograft (Bio-Oss) for the treatment of intrabony periodontal defects in humans. lnt J Periodontics Restorative Dent 2002; 22: 259-67
  48. Cardaropoli G., Leonhardt AS. Enamel matrix proteins in the treatment of deep intrabony defects. J Periodontol 2002; 73 :501-4 https://doi.org/10.1902/jop.2002.73.5.501
  49. Velasquez-Plata D., Scheyer ET., Mellonig JT. Clinical comparison of an enamel matrix derivative used alone or in combination with a bovine-derived xenograft for the treatment of periodontal osseous defects in humans. J Periodontol 2002; 73: 433-40 https://doi.org/10.1902/jop.2002.73.4.433
  50. Scheyer ET., Velasquez-Plata D., Brunsvold MA., Lasho DJ., Mellonig JT. A clinical comparison of a bovine-derived xenograft used alone and in combination with enamel matrix derivative for the treatment of periodontal osseous defects in humans. J Periodontol 2002; 73: 423-32 https://doi.org/10.1902/jop.2002.73.4.423
  51. Pietruska MD., Pietruski JK.. Stokowska W. Clinical and radiographic evaluation of periodontal therapy using enamel matrix derivative(Amdogain). Rocz Akad Med Bialymst 2001:46: 198-208
  52. Okuda K., Momose M., Miyazaki A., Murata M., Yokoyama S., Yonezawa Y., Wolff LF. Enamel matrix derivative in the treatment of human intrabony osseous defects. J Periodontol 2000; 71: 1821-8 https://doi.org/10.1902/jop.2000.71.12.1821
  53. Parashis A., Tsiklakis K. Clinical and radiographic findings following application of enamel matrix derivative in the treatment of intrabony defects. A series of case reports. J Clin Periodontol 2000; 27: 705-13 https://doi.org/10.1034/j.1600-051x.2000.027009705.x
  54. Lekovic V., Camargo PM., Weinlaender M., Nedic M., Aleksic Z., Kenney EB. A comparison between enamel matrix proteins used alone or in combination with bovine porous bone mineral in the treatment of intrabony periodontal defects in humans. J Periodontol 2000:71:1110-6 https://doi.org/10.1902/jop.2000.71.7.1110
  55. Schupbach P.. Gaberthuel T., Lutz F., Guggenheim B. Periodontal repair or regeneration : structures of different types of new attachment. J Periodontal Res. 1993 Jul;28:281-93 https://doi.org/10.1111/j.1600-0765.1993.tb02095.x