The study for the mouse cerebellum developments irradiated with γ-ray during embryogenesis

감마선 조사에 의한 발생과정 중인 마우스의 소뇌발달에 관한 연구

  • Park, Il-Kwon (College of the Veterinary Medicine, Chung Nam National University) ;
  • Lee, Kyoung-Youl (College of the Veterinary Medicine, Chung Nam National University) ;
  • Park, O-Sung (College of the Veterinary Medicine, Chung Nam National University) ;
  • Kim, Sung-Hwa (College of the Veterinary Medicine, Chung Nam National University) ;
  • Lee, Guen-Jwa (Chung Nam Veterinary Service Laboratory) ;
  • Lee, Kang-Yi (College of Oriental Medicine, Daejeon University) ;
  • Min, Tae-Sun (Department of Life Science, KOSEF) ;
  • Yook, Hong-Sun (Department of Food and Nutrition, College of Home Economics) ;
  • Byun, Myung-Woo (Korea Atomic Energy Research Institute, Team of Radiation Food and Biotechnology) ;
  • Kim, Moo-Kang (College of the Veterinary Medicine, Chung Nam National University)
  • 박일권 (충남대학교 수의과대학) ;
  • 이경열 (충남대학교 수의과대학) ;
  • 박오성 (충남대학교 수의과대학) ;
  • 김성화 (충남대학교 수의과대학) ;
  • 이근좌 (충남축산위생연구소) ;
  • 이강이 (대전대학교 한의과대학) ;
  • 민태선 (한국과학재단) ;
  • 육홍선 (충남대학교 생활과학대학 식품영양학과) ;
  • 변명우 (한국원자력연구소 방사선식품생명공학연구팀) ;
  • 김무강 (충남대학교 수의과대학)
  • Accepted : 2005.05.18
  • Published : 2005.06.30

Abstract

In order to study about the lobule and layer formation and cell migration of the mouse cerebellum from at the birth to 15th day effected by 2.5, 5 and 10 Gy r-raddiation at the 19th pregnancy. The routine tissue preparation and staining procedure, Immunohistochemical staining method by the several antibody and western brotting method were utilized from the birth to the15th day. The results were as followings. 1. The body and cerebellum weights were more slowly increase of the the 2.5 Gy, 5 Gy and 10 Gy irradiation group compare to the control group, and the health condition of the 2.5 Gy group was a little bad. but the 10 Gy group was more severe and begun to die from the 12th day after birth. 2. The thickness, proliferation and migration of the 2.5, 5 and 10 Gy irradiated external granular cells from the maginal zone to the medullary area forming the molecular layer from the 6th day to the 15th day after birth were thinner, weaker and more slower according to the radiated dosages than the control group in the cresyl violet staining. 3. The proliteration, migration and lobulation of the 5 Gy radiated groups from the first day to the 15th day after birth were more weak, incomplete and irregular shape in the immunostaining with Dab, Cdk5, P35, calbindin and Zebrin antibody. 4. In the western blotting analysis using the Reelin, Dab, Cdk5 and P35 antibody. The Bands were in the 60 KD, 80 KD, 33 KD and 35 KD, and there were no differences between the control and irradiated groups in the molecular band except the Reelin. 5. As a results, the proliferation and migration of the outer granular and purkinje cells, and lobulation of the cerebellum by the several dosaege of the ${\gamma}$-ray radiation were proportionally incomplete according to dosage.

Keywords

References

  1. Alcantara S, Ruiz M, D'Arcangelo G, Ezan F, de Lecea L, Curran T, Sotelo C, Soriano E. Regional and cellular patterns of reelin mRNA expression in the forebrain of the developing and adult mouse. J Neurosci 1998, 18, 777-779
  2. Altman J, Bayer SA. Prenatal development of the cerebellar system in the rat. I. Cytogenesis and histogenesis of the deep nuclei and the cortex of the cerebellum. J Comp Neurol 1978, 179, 23-48 https://doi.org/10.1002/cne.901790104
  3. Alvarado-Mallar RM, Martinez S, Lance-Jones CC. Pluripotentiality of the 2-day-old avian germinative neuroepithelium. Dev Biol 1990, 139, 75-88 https://doi.org/10.1016/0012-1606(90)90280-V
  4. Alvarez Otero R, Sotelo C, Alvarado-Mallar RM. Chick/quail chimeras with partial cerebellar grafts: an analysis of the origin and migration of cerebellar cells. J Comp Neurol 1993, 333, 597-615 https://doi.org/10.1002/cne.903330411
  5. Angaut P, Bowsher D. Ascending projections of the medial cerebellar (fastigial) nucleus. An experimental study in the cat. Brain Res 1970, 24, 49-68 https://doi.org/10.1016/0006-8993(70)90273-8
  6. Asano M, Gruss P. Pax-5 is expressed at the midbrainhindbrain boundary during mouse development. Mech Dev 1992, 39, 29-39 https://doi.org/10.1016/0925-4773(92)90023-D
  7. Bourrat F, Sotelo C. Neuronal migration and dendritic maturation of the medial cerebellar nucleus in rat embryos: an HRP in vitro study using cerebellar slabs. Brain Res 1986, 378, 69-85 https://doi.org/10.1016/0006-8993(86)90287-8
  8. Cajal SR. Histologie du Systeme Nerveux de l'Homme et des Vertebres, vol. II. A. Maloine, Paris. 1911
  9. Choi HU, Lee SK, Shim NR, Lee HW, Baek SY, Kim JB, Kim BS, Yoon S. Expression of the ED3 Antigen and its upregulation after Cyclophosphamide Treatment on Basal Epithelial Cells of Rat Stratified Squamous Epithelium. Korean J Anat 1993, 36, 1-10
  10. Constantino Sotelo. Cellular and genetic regulation of the development of the cerebellar system. Prog Neurobiol 2004, 72, 295-339 https://doi.org/10.1016/j.pneurobio.2004.03.004
  11. Crossley PH, Martinez S, Martin GR. Midbrain development induced by FGF8 in the chick embryo. Nature 1996, 380, 66-68 https://doi.org/10.1038/380066a0
  12. D'Arcangelo G, Miao GG, Chen SC, Soares HD, Morgan JI, Curran T. A protein related to extracellular matrix proteins deleted in the mouse mutant reeler. Nature 1995, 374, 719-723 https://doi.org/10.1038/374719a0
  13. D'Arcangelo G, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Curran T. Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal antibody. J Neurosci 1995, 17, 23-31
  14. Das GD. Experimental analysis of embryogenesis of cerebellum in rat I Subnormal growth following X-ray irradiation on day 15 of gestation. J Comp Neurol 1977, 176, 419-434 https://doi.org/10.1002/cne.901760308
  15. Davis CA, Noble-Topham SE, Rossant J, Joyner AL. Expression of the homeo box-containing gene En-2 delineates a specific region of the developing mouse brain. Genes Dev 1988, 2, 361-371 https://doi.org/10.1101/gad.2.3.361
  16. De Camilli P, Miller PE, Levitt P, Walter U, Greengard P. Anatomy of cerebellar Purkinje cells in the rat determined by a specific immunohistochemical marker. Neuroscience 1984, 11, 761-817 https://doi.org/10.1016/0306-4522(84)90193-3
  17. Gardner CA, Barald KF. The cellular environment controls the expression of engrailed-like protein in the cranial neuroepithelium of quail-chick chimeric embryos. Development 1991, 113, 1037-1048
  18. Gardner CA, Darnell DK, Poole SJ, Ordahl CP, Barald KF. Expression of an engrailed-like gene during development of the early embryonic chick nervous system. J Neurosci Res 1988, 21, 426-437 https://doi.org/10.1002/jnr.490210234
  19. Hallonet ME, Teillet MA, Le Douarin NM, A new approach to the development of the cerebellum provided by the quail-chick marker system. Development 1990, 108, 19-31
  20. Heikinheimo M, Lawshe A, Shackleford GM, Wilson DB, MacArthur CA. Fgf-8 expression in the post-gastrulation mouse suggests roles in the development of the face, limbs and central nervous system. Mech Dev 1994, 48, 129-138 https://doi.org/10.1016/0925-4773(94)90022-1
  21. Hidalgo-Sanchez M, Mille S, Simeone A, Alvarado-Mallart RM. Comparative analysis of Otx2, Gbx2, Pax2, Fgf8 and Wnt1 gene expressions during the formation of the chick midbrain/hindbrain domain. Mech Dev 1999, 81, 175-178 https://doi.org/10.1016/S0925-4773(98)00224-X
  22. Hidalgo-Sanchez M, Simeone A, Alvarado-Mallart RM. Fgf8 and Gbx2 induction concomitant with Otx2 repression is correlated with midbrain-hindbrain fate of caudal prosencephalon. Development 1999, 126, 3191-3203
  23. Hockfield S. A Mab to a unique cerebellar neuron generated by immunosuppression and rapid immunization. Science 1987, 237, 67-70 https://doi.org/10.1126/science.3603010
  24. Hunter KE, Hatten ME. Radial glial cell transformation to astrocytes is bidirectional: regulation by a diffusible factor in embryonic forebrain Proc Natl. Acad Sci USA 1995, 92, 2061-2065
  25. Ito M. The Cerebellum and Neural Control. Raven Press, New York, 1984
  26. Jeong YG, Kim MK, Hawkes R. Ectopic expression of tyrosine hydroxylase in zebrin Immunoreactive Purkinje cells in the cerebellum of the ataxic mutan mouse, pogo. Dev Brain Res 2001, 129, 201-209 https://doi.org/10.1016/S0165-3806(01)00212-7
  27. Jeong YG, Lee KY, Lee BC, Lee NS, Lee KY, Won MH. Fukui Y. Postnatal changes of cyclin-dependent kinase 5 activator expression in the developing rat cerebellum. Anat Histo Embryol 2005, 34, 20-26
  28. Laine J, Axelrad H. Morphology of the Golgiimpregnated Lugaro cell in the rat cerebellar cortex: a reappraisal with a description of its axon. J Comp Neurol 1996, 375, 618-640 https://doi.org/10.1002/(SICI)1096-9861(19961125)375:4<618::AID-CNE5>3.0.CO;2-4
  29. Laine J, Axelrad H. The candelabrum cell: a new interneuron in the cerebellar cortex. J Comp Neurol 1994, 339, 159-173 https://doi.org/10.1002/cne.903390202
  30. Laine J, Axelrad H. Extending the cerebellar Lugaro cell class. Neuroscience 2002, 115, 363-374 https://doi.org/10.1016/S0306-4522(02)00421-9
  31. Mallet J, Christen R, Changeux JP. Immunological studies on the Purkinje cells from rat and mouse cerebella. I. Evidence for antibodies characteristic of the Purkinje cells. Dev Biol 1979, 72, 308-319 https://doi.org/10.1016/0012-1606(79)90120-9
  32. Mallet J, Huchet M, Pougeois R, Changeux JP. Anatomical, physiological and biochemical studies on the cerebellum from mutant mice III Protein differences associated with the weaver, staggerer and nervous mutations. Brain Res 1976, 103, 291-312 https://doi.org/10.1016/0006-8993(76)90800-3
  33. Mariani J, Crepel F, Mikoshiba K, Changeux JP, Sotelo C. Anatomical, physiological and biochemical studies of the cerebellum from Reeler mutant mouse Philos Trans R Soc Lond B Biol Sci 1977, 281, 1-28 https://doi.org/10.1098/rstb.1977.0121
  34. Marin F, Puelles L. Patterning of the embryonic avian midbrain after experimental inversions: a polarizing activity from the isthmus. Dev Biol 1994, 163, 19-37 https://doi.org/10.1006/dbio.1994.1120
  35. Martinez S, Alvarado-Mallart RM. Rostral cerebellum originates from the caudal Portion of the so-called 'mesencephalic' vesicle: a study using chick/quail chimeras. Eur J Neurosci 1989, 1, 549-560 https://doi.org/10.1111/j.1460-9568.1989.tb00362.x
  36. Martinez S, Wassef M, Alvarado-Mallart RM. Induction of a mesencephalic phenotype in the 2-dayold chick prosencephalon is preceded by the early expression of the homeobox gene en. Neuron 1991, 6, 971-981 https://doi.org/10.1016/0896-6273(91)90237-T
  37. Miale IL, Sidman RL. An autoradiographic analysis of histogenesis in the mouse cerebellum. Expl Neurol 1961, 4, 277-296 https://doi.org/10.1016/0014-4886(61)90055-3
  38. Mugnaini E, Floris A. The unipolar brush cell: a neglected neuron of the mammalian cerebellar cortex. J Comp Neurol 1994, 339, 174-180 https://doi.org/10.1002/cne.903390203
  39. Nakamura H, Nakano KE, Igawa HH, Takagi S, Fujisawa H. Plasticity and rigidity of differentiation of brain vesicles studied in quail-chick chimeras. Cell Differ 1986, 19, 187-193 https://doi.org/10.1016/0045-6039(86)90095-3
  40. Pierce ET. Histogenesis of the deep cerebellar nuclei in the mouse: an autoradiographic study. Brain Res 1975, 95, 503-518 https://doi.org/10.1016/0006-8993(75)90124-9
  41. Pons S, Marti E. Sonic hedgehog synergizes with the extracellular matrix prote in vitro nectin to induce spinal motor neuron differentiation. Development 2000, 127, 333-342
  42. Rakic P. Neuron-glia relationship during granule cell migration in developing cerebellar cortex, A Golgi and electronmicroscopic study in Macacus Rhesus. J Comp Neurol 1971, 141, 283-312 https://doi.org/10.1002/cne.901410303
  43. Rowitch DH, McMahon AP. Pax-2 expression in the murine neural plate precedes and encompasses the expression domains of Wnt-1 and En-1. Mech Dev 1995, 52, 3-8 https://doi.org/10.1016/0925-4773(95)00380-J
  44. Simeone A, Acampora D, Gulisano M, Stornaiuolo A, Boncinelli E. Nested expression domains of four homeobox genes in developing rostral brain. Nature 1992, 358, 687-690 https://doi.org/10.1038/358687a0
  45. Song DL, Chalepakis G, Gruss P, Joyner AL. Two Pax-binding sites are required for early embryonic brain expression of an Engrailed-2 transgene. Development 1996, 122, 627-635
  46. Soriano E, Alvarado-Mallart RM, Dumesnil N, Del Rio JA, Sotelo C. Cajal-Retzius cells regulate the radial glia phenotype in the adult and developing cerebellum and alter granule cell migration. Neuron 1997, 18, 563-577 https://doi.org/10.1016/S0896-6273(00)80298-6
  47. Terrazas R. Notas sobre la neuroglia del cerebelo y el crecimiento de los elementos nerviosos. Revista Trimestral Micrografica 1897, 2, 49-65
  48. Trommsdorff M, Gotthardt M, Hiesberger T, Shelton J, Stockinger W, Nimpf J, Hammer RE, Richardson JA, Herz J. Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2. Cell 1999, 97, 689-701 https://doi.org/10.1016/S0092-8674(00)80782-5
  49. Wassarman KM, Lewandoski M, Campbell K, Joyner AL, Rubenstein JL, Martinez S, Martin GR. Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development 1997, 124, 2923-2934
  50. Wilkinson DG, Bailes JA, McMahon AP. Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo. Cell 1987, 50, 79-88 https://doi.org/10.1016/0092-8674(87)90664-7
  51. Wingate RJ, Hatten ME. The role of the rhombic lip in avian cerebellum development. Development 1999, 126, 4395-4404
  52. Yang HJ, Lee WY, Yoon H, Gil YG, Park KA, Lee HY. Morphological analysis of the Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the peripheral Nervous System of Mouse. Korean J Anat 2002, 35, 187-194
  53. Zhang L, Goldman JE. Generation of cerebellar interneurons from dividing progenitors in white matter. Neuron 1996, 16, 47-5 https://doi.org/10.1016/S0896-6273(00)80022-7