Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Proteome analysis of chickens fed with tissue culture medium waste after harvest of Korean wild ginseng

산삼 배양액을 급여한 육계에서 근육의 프로테옴 분석

  • Seol, Jae-Won (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Hwang, In Ho (National Livestock Research Institute) ;
  • Chae, Joon-Seok (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kang, Hyung-Sub (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Ryu, Kyeong-Seon (Department of Animal Resources and Biotechnology, Chonbuk National University) ;
  • Kang, Chun-Seong (E&T Co. Ltd.) ;
  • Park, Sang-Youel (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University)
  • 설재원 (전북대학교 수의과대학 생체안전성연구소) ;
  • 황인호 (축산기술연구소) ;
  • 채준석 (전북대학교 수의과대학 생체안전성연구소) ;
  • 강형섭 (전북대학교 수의과대학 생체안전성연구소) ;
  • 류경선 (농업생명과학대학 동물자원과학부) ;
  • 강춘성 ((주)이앤티) ;
  • 박상열 (전북대학교 수의과대학 생체안전성연구소)
  • Accepted : 2005.05.24
  • Published : 2005.06.30
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Abstract

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).

Keywords

References

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