Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Development of the immunochromatographic strip for the rapid detection of Listeria spp.

Listeria 속균 신속 검출을 위한 면역크로마토그라피 킷트 개발

  • Jung, Byeong-yeal (Veterinary Pharmacology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Jung, Suk-chan (Livestock Products Standard Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Kim, Jong-man (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry)
  • 정병열 (농림부 국립수의과학검역원 동물약품과) ;
  • 정석찬 (농림부 국립수의과학검역원 축산물규격과) ;
  • 김종만 (농림부 국립수의과학검역원 세균과)
  • Accepted : 2005.05.20
  • Published : 2005.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

We developed the one-step strip based on an immunochromatographic (IC) assay for the rapid detection of Listeria spp. Genus-specific monoclonal antibody to flagella of L. monocytogenes was conjugated with 40 nm colloidal gold particles which were prepared in our laboratory. The specificity of the IC strip was tested with pure cultured bacteria. All strains of the genus of Listeria spp. yielded positive reactions and 12 strains of non-Listeria were negative, resulting in a specificity of 100%. L. monocytogenes was artificially inoculated in raw pork macerated with listeria enrichment broth. And then it was 10-fold diluted from $8.7{\times}10^6$ to 8.7 CFU/ml. L. monocytogenes could be detected at a minimum of $8.7{\times}10^5CFU/ml$ before enrichment, $8.7{\times}10^2CFU/ml$ after primary enrichment and 8.7 CFU/ml after secondary enrichment, respectively. These results indicated that the IC strip exhibited high specificity and sensitivity in the detection of Listeria spp.

Keywords

References

  1. 백선영, 곽효선, 차진, 박성국, 임순영, 김형일, 박선희, 김창민. 냉동식품중 Listeria monocytogenes의 분포 조사 및 시험방법에 관한 연구. 식품의약품안전청 연보 1997, 1, 31-37
  2. 정병열, 임현숙, 김봉환. 가축의 장내용물에서 Listeria 속균의 분포도 조사. 한국수의공중보건학회지 2003, 27, 41-46
  3. 정병열, 임현숙, 정석찬. Listeria monocytogenes의 신속검출을 위한 선택배지 및 multiplex PCR 기법 개발. 대한수의학회지 2003, 43, 231-237
  4. Chiao DJ, Shyu RH, Hu CS, Chiang HY, Tang SS. Colloidal gold-based immunochromatographic assay for detection of botulinum neurotoxin type B. J Chromatogr 2004, 809, 37-41 https://doi.org/10.1016/j.jchromb.2004.05.033
  5. Conner DE, Scott VN, Bernard DT. Growth, inhibition and survival of Listeria monocytogenes as affected by acidic conditions. J Food Prot 1990, 53, 652-655 https://doi.org/10.4315/0362-028X-53.8.652
  6. Duarte G, Vaz-Velho M, Capell C, Gibbs P. Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains. Int J Food Microbiol 1999, 52, 163-168 https://doi.org/10.1016/S0168-1605(99)00121-X
  7. Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev 1991, 55, 476-511
  8. Geoghegan WD, Ackerman GA. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem 1977, 25, 1187-1200 https://doi.org/10.1177/25.11.21217
  9. Gray ML, Killinger AH. Listeria monocytogenes and listeric infections. Bacteriol Rev 1966, 30, 309-382
  10. Kerdahi KF, Istafanos PF. Rapid determination of Listeria monocytogenes by automated enzyme-linked immunoassay and nonradioactive DNA probe. J AOAC Int 2000, 83, 86-88
  11. Kim SH. Development of the rapid immunoassay methods for the screening of Listerial contamination. PhD Thesis. Cheju National University. 2004
  12. Muller JD, Wilks CR, O'Riley KJ, Condron RJ, Bull R, Mateczun A. Specificity of an immunochromatographic test for anthrax. Aust Vet J 2004, 82, 220-222 https://doi.org/10.1111/j.1751-0813.2004.tb12682.x
  13. Paek SH, Lee CW, Yoon SH, Kwon OH, Kim YN. Performance control strategies of one-step immunochromatographic assay system for Salmonella typhimurium. Anal Lett 1999, 32, 335-360 https://doi.org/10.1080/00032719908542824
  14. Peel M, Donachie W, Shaw A. Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting. J Gen Microbiol 1988, 134, 2171-2178
  15. Smits HL, Abdoel TH, Solera J, Clavijo E, Diaz R. Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for rapid serodiagnosis of human brucellosis. Clin Diagn Lab Immunol 2003, 10, 1141-1146
  16. Waak E, Tham W, Danielsson-Tham M-L. Comparison of the ISO and IDF methods for detection of Listeria monocytogenes in blue veined cheese. Int Dairy J 1999, 9, 149-155 https://doi.org/10.1016/S0958-6946(99)00036-9
  17. Yu KY, Noh Y, Chung M, Park HJ, Lee N, Youn M, Jung BY, Youn BS. Use of monoclonal antibodies that recognize p60 for identification of Listeria monocytogenes. Clin Diagn Lab Immunol 2004, 11, 446-451