Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Nucleotide sequence analysis and expression of NSP4 gene of avian rotavirus

조류 로타바이러스의 NSP4 유전자 염기서열분석 및 발현

  • Shin, In-ho (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Seung-chul (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Won-yong (College of Medicine, Chungang University) ;
  • Kang, Shien-young (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
  • 신인호 (충북대학교 수의과대학 동물의학연구소) ;
  • 이승철 (충북대학교 수의과대학 동물의학연구소) ;
  • 김원용 (중앙대학교 의과대학) ;
  • 강신영 (충북대학교 수의과대학 동물의학연구소)
  • Accepted : 2005.05.27
  • Published : 2005.06.30
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Abstract

The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene of avian rotavirus (AvRV-1, AvRV-2) was analyzed and expressed using baculovirus expression system. The sequence data indicated that the NSP4 gene of AvRV-1 and AvRV-2 were 727 bases in length, encoded one open reading frame of 169 amino acids beginning at base 41 and terminating at base 550, and had two glycosylation sites. Nucleotide sequences of NSP4 gene of AvRV-1 and AvRV-2 exhibited a high degree of homology ($88.1{\pm}7.6%$) with avian rotaviruses, namely Ty1, Ty3 and PO-13. Phylogenetic analysis showed that AvRV-1 and AvRV-2 belonged to genotype NSP4[E], which is widely found in group A avian rotaviruses. The baculovirus-expressed NSP4 migrated at 20-28 kDa and reacted with NSP4-specific antiserum by FA and Western blot. Furthermore, it was found to be a glycoprotein by using tunicamycin, which is a specific inhibitor of N-linked glycosylation.

Keywords

References

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