Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Bovine papillomavirus detection from bovine teats using immunohistochemistry and electronmicroscopy

면역조직화학염색 및 전자현미경검사를 이용한 소 유두로부터 소유두종바이러스 검출

  • Bae, You-Chan (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Yoon, Soon-Seek (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Park, Jung-Won (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Lee, Cheong-San (Chungbuk Livestock and Veterinary Research Institute) ;
  • Jean, Yong-Hwa (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry) ;
  • Kang, Mun-Il (College of Veterinary Medicine, Chonnam National University)
  • 배유찬 (국립수의과학검역원 병리과) ;
  • 윤순식 (국립수의과학검역원 병리과) ;
  • 박중원 (국립수의과학검역원 병리과) ;
  • 이청산 (충청북도 축산위생연구소) ;
  • 진영화 (국립수의과학검역원 병리과) ;
  • 강문일 (전남대학교 수의과대학)
  • Accepted : 2005.05.24
  • Published : 2005.06.30
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Abstract

We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26. Electronmicroscopically, in Holstein papilloma cases, BPV particles was detected in 31 heads (39.2%) of 79. This study revealed that papilloma was very prevalent in holstein teats, Korea and the prevalence of papilloma in Holstein was 8 times higher than that of Korean native cattle. Moreover, it was needed to consider gross and histopathological lesions to confirm BPV infection on teats because the sensitivity of immunohistochemistry and electronmicroscopy was not high.

Keywords

References

  1. Bagdonas V, Olson C. Observations on the Epizootiology of Cutaneous Papillomatosis(Warts) of Cattle. J Am Vet Med Ass 1953, 122, 393-397
  2. Barthold SW, Koller LD, Olson C, Studer E, Holtan A. Atypical Warts in Cattle. J Am Vet Med Ass 1974, 165, 276-280
  3. Bloch N, Sutton RH, Breen M, Spradbrow PB. Identification of papillomaviruses in scrapings from bovine warts by use of the polymerase chain reaction. Vet Res Commun 1997, 21, 63-68 https://doi.org/10.1023/B:VERC.0000009702.65202.3b
  4. Campo MS. Bovine Papillomatosis and Cancer. Vet J 1997, 154, 175-188 https://doi.org/10.1016/S1090-0233(97)80019-6
  5. Debey BM, Bagladi-Swanson M, Kapil S, Oehme FW. Digital papillomatosis in a confined Beagl. J Vet Diagn Invest 2001, 13, 346-348 https://doi.org/10.1177/104063870101300412
  6. Gerdes G, Lugt J. Electronmicroscopic evidence of a papillomavirus and a parapoxvirus in the same lesion. Vet Rec 1991, 128, 594-595 https://doi.org/10.1136/vr.128.25.594
  7. Lindholm I, Murphy J, O'Neil BW, Campo MS, Jarrett WF. Papilllomatosis of the teats and udder of cattle and their casual viruses. Vet Rec 1984, 115, 574-577 https://doi.org/10.1136/vr.115.22.574
  8. Meischke HR. A Survey of bovine teat papillomatosis. Vet Rec 1979, 104, 28-31 https://doi.org/10.1136/vr.104.2.28
  9. Nicholls P, Stanley M. The immunology of animal papillomaviruses. Vet Immunol Immunopathol 2000, 73, 101-127 https://doi.org/10.1016/S0165-2427(99)00165-8
  10. Olson RO, Olson C, Easterday BC. Papillomatosis of the bovine teat(mammary papilla). Am J Vet Res 1982, 43, 2250-2252
  11. Otten N, Tscharner CV, Lazary S, Antczak DF, Gerber H. DNA of bovine papillomavirus type 1 and 2 in equine sarcoids: PCR detection and direct sequencing. Arch Virol 1993, 132, 121-131 https://doi.org/10.1007/BF01309847
  12. Sundberg JP, Junge RE, Lancaster WD. Immunoperoxidase localization of papillomaviruses in hyperplastic and neoplastic epithelial lesions of animals. Am J Vet Res 1984, 45, 1441-1446
  13. Sundberg JP, Ranst MV, Montali R, Homer BL, MIller WH, Rowland PH, Scott DW, England JJ, Dunstan RW, Mikaelian I, Jensen AB. Feline Papillomas and Papillomaviruses. Vet Path 2000, 37, 1-10 https://doi.org/10.1354/vp.37-1-1
  14. Timoney JF, Gillespie JH, Scott FW, Barlough JE. Hagan and Bruner's Microbiology and Infectious Diseases of Domestic Animals, 8th ed. pp. 523-526, 584-585. Comstock Publishing Associates, Ithaca and London, 1988