Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Effects of bone healing capacity by autologous fibrin glue in experimental bone defect dogs

개에서 자가 fibrin glue가 골 결손치유에 미치는 영향

  • Lee, Jong-il (College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Ha-na (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Nam-soo (College of Veterinary Medicine, Chonbuk National University)
  • Accepted : 2005.05.20
  • Published : 2005.06.30
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Abstract

This study was investigated of the bone healing capacity by autologous fibrin glue in experimental bone defect dogs. The autologous fibrin glue manufactured just before the experiment was mixed with the concentrated fibrinogen from whole blood of the experimental dog and bovine thrombin. The experimental group was constituted with seven dogs. The experimental osteotomy was performed 5 mm length in bilateral region of proximal diaphyseal fibulae. The defected regions of experimental group were filled with the autologous fibrin glue by duploject. The experimental regions had been radiographed biweekly for 16 weeks to observe new bone formation and union. Bone alkaline phophatase (BALP) in all groups was evaluated biweekly till the end of the experiment to determine osteoblast activities. New bone formation had been observed in five regions of three dogs at four weeks after the experimental treatment and in two regions of one dog at ten weeks. The other seven regions of the experimental group and control group were not observed new bone formation until the end of the experiment. BALP value in four dogs observed new bone formation was increased to 97.10 IU/L (453.96%) at two weeks after the experimental treatment. The results of this experiment were suggested that the autologous fibrin glue was moderately effective in new bone formation in dogs.

Keywords

References

  1. 김남수, 최인혁. 골형성 지표로써 bone alkaline phosphatase(BALP)와 TALP치에 대한 BALP치 비율 (B/T)의 응용. 대한수의학회지 1999, 39, 1197-1209
  2. 박문서, 차창일. 황산암모늄 침강법에 의해 제조된 자가 Fibrin접착제에 대한 생화학적 연구. 한이인지 1989, 32, 547-557
  3. 양규현, 김남현. 자가 Fibrin으로 고정하여 이식한 자가골수세포가 골결손 치유에 미치는 영향. 대한정형외과학회지 1991, 26, 1289-1304
  4. 조성진, 김남수, 최인혁. 정상적인 개에서의 Serum Bone Alkaline Phosphatase의 활성치. 한국임상수의학회지 1997, 14, 65-69
  5. 최인혁, 이종일. 동결건조한 산양뼈의 개이식 효과. 한국임상수의학회지 1998, 15, 442-449
  6. Albrektsson T, Bach A, Edshage S, Jonsson A. Fibrin adhesive system (FAS) influence on bone healing rate. Acta Orthop Scand 1982, 53, 757-763 https://doi.org/10.3109/17453678208992288
  7. Arbes H, Bosch P, Lintner F, Salzer M. First clinical expierience with heterologous cancellous bone grafting combined with the fibrin adhesive system (F.A.S.). Arch Orthop Traumat Surg 1981, 98, 183-188 https://doi.org/10.1007/BF00632975
  8. Arce AA, Garin DM, Garcia VM, Jansana JP. Treatment of radial head fracture using a fibrin adhesive seal. J Bone Joint Surg (Br). 1995, 77, 422-424
  9. Berh W, Barnert J. Quantification of bone alkaline phosphatase in serum by precipitation with wheat-germ lectin : a simplified method and its clinical plausibility. Clin Chem 1986, 10, 1960-1966
  10. Bosch P, Lintner F, Arbes H, Brand G. Experimental investigations of the effect of the fibrin adhesive on the Kiel heterologous bone graft. Arch Orthop Traumat Surg 1980, 96, 177-185 https://doi.org/10.1007/BF00457781
  11. Brittberg M, Sjogren-Jansson E, Lindahl A, Peterson L. Influence of fibrin sealant (Tisseel) on osteochondral defect repair in the rabbit knee. Biomaterials 1997, 18, 235-242 https://doi.org/10.1016/S0142-9612(96)00117-2
  12. DePalma L, Criss VR, Luban NLC. The preparation of fibrinogen concentrate for use as fibringlue by four different methods. Transfusion 1993, 33, 712-720
  13. Fishman WH. Perspectives on alkalin phosphatase isoenzymes. Am J Med 1974, 54, 617-650
  14. Isogai N, Landis WJ, Mori R, Gotoh Y, Gerstenfeld LC, Upton J, Vacanti JP. Experimental use of fibrin glue to induce site-directed osteogenesis from cultured periosteal cells. Plast Reconstr Surg 2000, 105, 953-963 https://doi.org/10.1097/00006534-200003000-00019
  15. Jarzem P, Harvey EJ, Shenker R, Hajipavlou A. The effect of fibrin sealant on spinal fusions using allograft in dogs. Spine 1996, 21, 1307-1312 https://doi.org/10.1097/00007632-199606010-00005
  16. Kania RE, Meunier A, Hamadouche M, Sedel L, Petite H. Addition of fibrin sealant to ceramic promotes bone repair: Long-term study in rabbit femoral defect model. J Biomed Mater Res 1998, 43, 38-45 https://doi.org/10.1002/(SICI)1097-4636(199821)43:1<38::AID-JBM4>3.0.CO;2-N
  17. Kaplonyi G, Zimmerman I, Frenyo AD, Farkas T, Nemes G. The use of fibrin adhesive in the repair of chondral and osteochondral injuries. Injury 1988, 19, 267-272 https://doi.org/10.1016/0020-1383(88)90043-5
  18. Knighton DR, Hunt TK, Thakral KK, Goodson III WH. Role of platelets and fibrin in the healing sequence Ann Surg 1982, 196, 379-388 https://doi.org/10.1097/00000658-198210000-00001
  19. Leung KS, Fung KP, Sher AHL, Li CK, Lee KM. Plasma bone specific alkaline phosphatase as an indicator of osteoblastic activity. J Bone Joint Surg 1993, 75, 288-292
  20. Lasa JrC, Hollinger J, Drohan W, MacPhee M. Delivary of demineralized bone powder by fibrin sealant. Plast Reconstr Surg 1995, 96, 1409-1417 https://doi.org/10.1097/00006534-199511000-00027
  21. Lucht U, Bunger C, Moller JT, Joyce F, Plenk JrH. Fibrin sealant in bone transplantation. Acta Orthop Scand 1986, 57, 19-24 https://doi.org/10.3109/17453678608993208
  22. Matsumoto K, Kohmura E, Kato A, Hayakawa T. Restoration of small bone defects at craniotomy using autologous bone dust and fibrin glue. Surg Neurol 1998, 50, 344-346 https://doi.org/10.1016/S0090-3019(98)00081-0
  23. Park MS, Cha CI. Biochemical aspects of autologous fibrin glue derived from ammonium sulfate preparation. Laryngoscope 1993, 103, 193-196 https://doi.org/10.1002/lary.5541030213
  24. Reiss RF, Oz MC. Autologous fibrin glue : production and clinical use. Transfusion Medicine Reviews 1996, 10, 85-92 https://doi.org/10.1016/S0887-7963(96)80085-X
  25. Rosalki SB, Foo AY. Two new methods for seperating and quantifying bone and liver alkaline phophatase isoenzymes in plasma. Clin Chem 1984, 7, 1182-1186
  26. Schwarz N, Redl H, Zeng L, Schlag G, Dinges HP, Eschberger J. Early osteoinduction in rats is not altered by fibrin sealant. Clinical Orthopaedics and Related Research 1993, 293, 353-359
  27. Yoshida H, Kamiya A. A quicker preparation method for autologous fibrin glue. Biol Pharm Bull 1998, 21, 1367-1370 https://doi.org/10.1248/bpb.21.1367