Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Incidence of micronuclei in lymphocytes of pig in the high background radiation area (Cheongwon-gun and Boeun-gun)

국내 자연 방사선 고준위 지역(청원군, 보은군) 사육 돼지의 림프구 미소핵 발생 평가

  • Lee, Hae-June (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Chang-Mo (Korea Institute of Radiological & Medical Science) ;
  • Kim, Se-Ra (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jin-Hee (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Joong-Sun (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Ill-Hwa (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Tae-Hwan (College of Veterinary Medicine, kyungbuk National University) ;
  • Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University) ;
  • Jo, Sung-Kee (Food Irradiation Team, Korea Atomic Energy Research Institute) ;
  • Choi, Soo-Yong (Korea Institute of Radiological & Medical Science) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2005.11.03
  • Published : 2005.12.29
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Abstract

Cytogenetic and hematological analysis was performed in peripheral blood obtained from pigs bred in the high background radiation areas (HBRA) (Cheongwon-gun and Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis-blocked (CB) lymphocytes at several doses were measured in three pigs. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the swine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of $y=0.0005404D^2+0.04237D+0.00833$ [y = number of MN/cytokinesis-blocked (CB) cells and D = irradiation dose in Gy]. MN rates per 1,000 CB lymphocytes of pig from the HBRA (Cheongwon-gun, Boeun-gun) and the control area were $6.70{\pm}2.36$, $9.00{\pm}3.50$ and $11.00{\pm}2.98$, respectively. The MN frequencies of CB lymphocytes from pigs bred in three areas means that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the pigs were bred in the HBRA or the control area.

Keywords

References

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