Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Expression of caveolin-3 as positive intracellular signaling regulator on the development of hypertrophy in cardiac tissues

  • Kim, Joo-Heon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Han, Jin (Cardiovascular & Metabolic Disease Center, College of Medicine, Inje University) ;
  • Kim, Yong-Kwon (College of Biomedical Science and Engineering, Inje University) ;
  • Yang, Young-Ae (College of Biomedical Science and Engineering, Inje University) ;
  • Hong, Yonggeun (Cardiovascular & Metabolic Disease Center, College of Medicine, Inje University)
  • Accepted : 2005.11.28
  • Published : 2005.12.29
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Abstract

We have examined distribution and expression of caveolin-3 (cav-3), one of three caveolin isoforms from 16-wks-old spontaneously hypertensive rats (SHR) compared with age-matched control wistar-kyoto (WKY) rats. The expression of cav-3 was increased, whereas expression of PKB/Akt and calcineurin (Cn) was not changed in cardiac tissues of SHR compared to WKY rats. Interestingly, expression of cav-3, PKB/Akt and Cn were decreased in plasma membrane fraction in SHR compared to WKY rats. In H9c2 cardiomyoblast cells treated with phenylephrine ($50{\mu}M$, 48hr) or isoproterenol ($10{\mu}M$, 48hr), the expression of cav-3 was markedly enhanced compared to nontreated cells. Upon immunofluorescence analysis, cav-3 was localized in plasma membrane of control H9c2 cells. However phenylephrine or isoproterenol treatment caused translocation of cav-3 to perinuclear region. These results suggest that cav-3 plays as positive regulators in the development of hypertrophy in cardiac tissues of SHR rats.

Keywords

References

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