Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Pie-establishment of Microwave-Assisted Extraction Conditions for Antioxidative Extracts from Cabbage

양배추의 항산화성 추출물 제조를 위한 마이크로웨브 추출조건 설정

  • Noh Jungeun (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Choi You-Kyoung (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Kim Hyun-Ku (Korea Food Research Institute) ;
  • Kwon Joong-Ho (Dept. of Food Science and Technology, Kyungpook National University)
  • Published : 2005.02.01
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Abstract

Microwave-assisted extraction (50 W, 2,450 MHz, MAE) with properties of selective heating and subsequent extraction for certain phytochemicals from natural materials was applied to pre-establish the extraction conditions for total yield total phenolics, and electron donating ability (EDA) from Brossica oleacea. The experiments with $50\%$ EtOH solvent showed that 20 mesh in particle size of cabbage flake $(moisture\;4.5\%)$ and 1:10 (g/mL) in the sample to solvent ratio for both raw $(moisture\;90.2\%)$ and flake cabbages were optimal for MAE efficiency. Under these conditions, total yield increased with extraction tim, which was highest for raw cabbage extract in $50\%\;EtOH$ solvent followed by $100\%\;EtOH$ and water. While that of flake cabbage extracts was highest in $50\%\;EtOH$ followed by water and $100\%\;EtOH$. The contents of total phenolics and EDA in extracts gradually increased after 3 min of MAE, which were highest when using $100\%\;EtOH$ solvent followed by $50\%\;EtOH$ and water in raw cabbage and $50\%\;EtOH$ followed by water and $100\%\;EtOH$ in flake cabbage, respectively.

양배추의 고부가가치 이용방안 연구의 일환으로 선택적 가열 및 추출특성이 알려진 마이크로파 에너지(50 W, 2,450 MHz)를 이용하여 양배추 추출물의 총 수율과 총 페놀 함량 및 전자공여능의 효율적 추출조건을 설정하였다. 에탄올$(50\%)$ 용매에서 건조 양배추의 입자 크기는 20 mesh 정도가 적당하였고, 시료와 용매비는 생체시료(수분 $90.2\%$)와 건조시료(수분 $4.5\%$) 모두에서 1:10(5 g/50 mL) 정도가 가장 타당한 것으로 나타났다. 추출용매의 영향에서는 생체시료에서는 $50\%$ 에탄올$>100\%$ 에탄올<물의 순으로, 건조시료에서는 $50\%$ 에탄올>물$>100\%$ 에탄올의 순으로 높은 추출수율을 보여주었다. 추출물의 총 페놀 함량과 전자공여능은 추출 3분 이후에는 거의 차이가 없는 경향을 보여주었고, 생양배추 시료에서는 $100\%$ 에탄올$>50\%$ 에탄올>물의 순으로, 건조 양배추 시료에서는 $50\%$ 에탄올>물$>100\%$ 에탄을 순으로 높은 함량을 보여주었다.

Keywords

References

  1. Lee, C.B. (1982) A pictorial book of the Korean flora. Hyangmunsa, p.559
  2. Owen R. F. (1996) Food chemistry - 3rd edition. Marcel Dekker, Inc., p.337-339
  3. Kim, M.R., Kim, J.H., Wi, D.S., Na, J.H. and Sok, D.E. (1999) Volatile sulfur compounds, proximate components, minerals, vitamin C content and sensory characteristics of the juices of kale and broccoli leaves. J. Korean Soc. Food Sci. Nutr., 28, 1201-1207
  4. Han, Y.B., Kim, M.R., Han, B.H. and Han, Y.N. (1987) Studies on anti-oxidant component of mustard leaf and seed. Kor. J. Pharmacogn, 18, 41-49
  5. Kim, M.R., Lee, K.J., Kim, J.H. and Sok, D.E. (1997) Determination of sulforaphane in cruciferous vegetables by SIM. Korean J. Food Sci. Technol., 29, 882-887
  6. Chin, H.W, Zeng, G. and Lindsay, R. (1986) Occurrence and flavour properties of sinigrin hydrolysis products in fresh cabbage. J. Food Sci., 61, 101-104 https://doi.org/10.1111/j.1365-2621.1996.tb14735.x
  7. Fahey, J.W. and Talalay, P. (1999) Antioxidant functions of sulforaphane a potent inducer of phase II detoxication enzymes. Food and Chem. Toxicol., 37, 973-979 https://doi.org/10.1016/S0278-6915(99)00082-4
  8. Spencer, G.F. and Daxenbichler, M.E. (1980) Gas chromatography-mass spectrometry of nitriles, isothiocyanates and oxazolidinethiones derived from cruciferous glucosinolates. J. Sci. Food Agric., 31, 359-367 https://doi.org/10.1002/jsfa.2740310406
  9. Kim, M.R., Lee, K.J. and Kim, H.Y. (1997) Effect of processing on the content of sulforaphane of broccoli. Korean J. Soc. Food Sci., 13, 44-47
  10. Kim, K.E., Lee, G.D. and Kwon, J.H. (2000) Preestablishment of microwave-assisted extraction under atmospheric pressure condition for ginseng components. Korean J. Food Sci. Technol., 32, 323-327
  11. Pare, J.R.J., Blanger, J.M.R. and Stafford, M.R. (1994) Microwave-assisted process: a new tool for the analytical laboratory. Trends Anal. Chem., 13, 176-184 https://doi.org/10.1016/0165-9936(94)87033-0
  12. Belanger, J.M.R., Belanger, A. and Pare, J.R.J. (1996) Microwave-assisted process$(MAP^{TM})$: Application to oleoresins of celery seeds and black pepper. Research articles of environmental technical centre, Environment Canada
  13. Lee, S.B., Lee, G.D. and Kwon, J.H. (1999) Optimization of extraction conditions for soluble ginseng components using microwave extraction system under pressure. J. Korean Soc. Food Sci. Nutr., 28, 409-416
  14. Kwon, J.H., Lee, G.D., Belanger, J.M.R. and Pare, J.R.J. (2003) Effect of ethanol concentration on the efficiency of extraction of ginseng saponins when using a microwave-assisted process (MAP). Int'l. J. Food Sci. Technol., 38, 615-622 https://doi.org/10.1046/j.1365-2621.2003.00688.x
  15. Kwon, J.H., Belanger, J.M.R., Pare, J.R.J. and Yaylayan, V.A. (2003) Application of microwave-assisted process (MAP) to the fast extraction of ginseng saponins. Food Research International, 36, 491-498 https://doi.org/10.1016/S0963-9969(02)00197-7
  16. Kwon, J.H., Belanger, J.M.R. and Pare, J.R.J. (2003) Optimization of microwave-assisted extraction (MAP) for ginseng components by response surface methodology. J. Agric, Food Chem., 51, 1807-1810 https://doi.org/10.1021/jf026068a
  17. Kwon, Y.J., Kim K.H. and Kim H.K. (2002) Changes of total polyphenol content and antioxidant activity of Ligularia fischeri extracts with different microwave-assisted extraction conditions. Korean J. Food Preserv., 9, 332-337
  18. Origin (1999) Origin tutorial manual, version 6.0. Microcal Software, Inc., Northampton, MA, USA
  19. Lee, E.J. (2004) Optimization of microwave-assisted extraction conditions for grape seed components. MS thesis, Kyungpook National University, Daegu, Korea
  20. Kim, K., Lee, G.D. and Kwon, J.H. (2000) Preestablishment of microwave-assisted extraction under atmospheric pressure condition for ginseng components. Korean J. Food Sci. Technol., 32, 323-327
  21. Lee, S.Y., Kang, M.J., Kwon, J.H., Shin, S.R., Lee, G.D. and Kim, K.S. (2003) Monitoring of total phenolics, electron donating ability and nitrite scavenging ability in microwave-assisted extraction for Angelica Nakai. Food Sci. Biotechnol., 12, 491-496
  22. Kwon, J.H., Choi, Y.H., Chung, H.W. and Lee, G.D. (2005) Selective extraction characteristics for saikosaponins from Bupleurum falcatum root using a microwave process. Int'l. J. Food Sci. Technol., in press
  23. Lee, M.H. (2002) Rapid analysis of pesticide residues in natural medicines by microwave-assisted extraction. MS thesis, Kyungpook National University, Daegu, Korea
  24. Kwon, J.H. (1998) High speed extraction of functional components from food and natural products using microwave-assisted process. Food Sci. Ind., 31, 43-55
  25. Zielinski, H. and Kozlowska, H. (2000) Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J. Agric. Food Chem., 48, 2008-2018 https://doi.org/10.1021/jf990619o
  26. Bertelli, D., Plessi, M. and Miglietta, F. (2004) Effect of industrial microwave treatment on the antioxidant activity of herbs and spices. Ital. J. Food Sci., 16,97-103