Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant Activity and Inhibitive Effects on Human Leukemia Cells of Edible Mushrooms Extracts

식용버섯 추출물의 항산화 활성 및 혈액암세포에 대한 저해효과

  • Kim Hyun Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Bae Joon-Tae (Dept. of Oriental Medical Food and Nutrition, Asia University) ;
  • Lee Ji-Won (Dept. of Food Science and Technology, Keimyung University) ;
  • Hwang Bo Mi-Hyang (Dept. of Food Science and Technology, Keimyung University) ;
  • Im Hyo Gwon (Dept. of Food Science and Technology, Keimyung University) ;
  • Lee In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 배준태 (아시아대학교 한방식품영양학과) ;
  • 이지원 (계명대학교 식품가공학과) ;
  • 황보미향 (계명대학교 식품가공학과) ;
  • 임효권 (계명대학교 식품가공학과) ;
  • 이인선 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터)
  • Published : 2005.02.01
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Abstract

The effect of 12 edible mushroom species on the antioxidant and cytotoxicity on cancer cells were studied Methanol extract of Lyophyllum ulmarium, Cordyceps militaris and Sarcodon aspratus showed $30\~60\%$ DPPH radical scavenging activity and $39\~53\%$ protective effects against the cytotoxicity of $B_2O_2$. Methanol extracts of Sarcodon aspratus, Lyophyllum ulmarium, Cordyceps militaris, Agaricus blazei and Ganoderma lucidum revealed high inhibitive activities in cytotoxicity on human leukemia tells such as promyelocytic leukemia cell (HL60) and histiocytic lymphoma cell (U937). Highest toxicity was observed against HL60 cells in Sarcodon aspratus methanol extract showing $70.5\%$ inhibition at 1mg/mL whereas Cordyceps militaris methanol extract showed $81.5\%$ inhibition against U937 cells. Most water extracts of edible mushrooms exhibited the lowest effect against HL60 and U937 cells compared to methanol extract. These extract did not show cytotoxic effects against human lymphocyte. Results revealed 5 kinds of edible murshroom (Cordyceps militaris, Agaricus blaxei, Lyophyllum ulmarium, Ganoderma lucidum and Sarcodon aspratus) have strong antioxidative and in vitro anticancer efforts.

일상생활에서 식용 또는 약용으로 이용되고 있는 12종의 버섯 추출물을 제조하여 이들 추출물의 항산화능 검색과 함께 인간유래의 혈액암 세포주 및 정상 임파구세포의 성장에 미치는 영향에 대하여 조사하였다. 그 결과, 향버섯, 번데기동충하초, 만가닥버섯, 아가리쿠스, 영지버섯, 표고버섯 메탄올 추출물에서 $30\~60\%$정도의 DPPH radical 소거 활성을 보였고, 목이버섯, 그물버섯, 새송이버섯에서 $10\~30\%$의 활성을 보였다. 또한 chinese hamster V79 cells에서 만가닥 버섯, 번데기동충하초, 향버섯 메탄올추출물의 경우 H2O2로 유도된 세포 독성에 대한 $39\~53\%$ 정도의 유의적인 생존율을 나타내었다. 그리고 인간유래 혈액암세포인 HL6O과 U937에 대해 향버섯, 만가닥버섯, 번데기동충하초, 아가리쿠스, 영지버섯 추출물들이 높은 증식 억제 활성이 보였다. 특히 버섯 메탄올추출물 1.0 mg/mL 처리시 HL 60에 대해 향버섯이 $70.5\%$로 가장 높은 저해율을, U937에 대해서는 번데기동충하초가 $81.5\%$의 가장 높은 저해율을 나타내었다. 그리고 버섯 열수추출물들은 메탄올추출물보다 낮은 저해 활성을 보였다. 그러나 모든 버섯추출물들은 인간의 정상 임파구세포에 대해 $95\%$ 이상의 높은 생존율을 나타내어, 정상 세포에 대한 성장 저해 효과가 없음을 보여주었다. 번데기동충하초, 아가리쿠스, 만가닥버섯, 영지버섯, 향버섯 추출물은 항산화 할성 및 혈액암 세포주에 대한 증식 억제 활성이 높음을 확인할 수 있었다.

Keywords

References

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