Analysis of Total Sugar by Extraction Condition and Material to Develope the Extraction Process of Ginseng Polysaccharide

인삼 다당체 추출 공정 개발을 위한 인삼의 추출 조건 및 원료에 따른 총당 변화

  • Jang Soon-Ae (Department of Food and Nutrition, Chungnam National University) ;
  • Moon Sook-Kyung (Department of Food and Nutrition, Chungnam National University)
  • 장순애 (충남대학교 식품영양학과) ;
  • 문숙경 (충남대학교 식품영양학과)
  • Published : 2005.08.01

Abstract

This study analyzed the extraction condition for large-scale extraction process to be used industrially. The total sugar content of 5-year Red ginseng in viewpoint of the ginseng materials was highest by $32\%$ and it of 5-year Keumsan ginseng was $31\%$. Therefore 5-year Keumsan ginseng was used by test sample. The next extraction condition, that is the total sugar content of the internal white among the parts of ginseng, the extraction efficiency under the condition of optimal temperature $80^{\circ}C$ and optimal extract time 6hrs, was highest. And the amount of total sugar extracted from ginseng treated with protease, ${\alpha}-amylase$ and cellulase was increased about $20\%$. Total sugar recovery in methods of alcohol concentration $70\%$ and freezer-dry method was highest.

인삼 속에 다량으로 함유되어 있는 다당체 성분인 진산에 대한 효능이 연구되고 있는 시점에서 산업적으로 이용할 수 있는 대량 추출공정을 위한 추출조건을 분석하였다. 인삼의 종류는 홍삼(5년근)이 $32\%$로 총당의 함량이 가장 높았으며 금산 수삼(5년근)은 $31\%$$1\%$ 정도 조금 적게 나타났다. 그러나 홍삼 제조 공정에 따른 효율을 비교해 본 결과 금산 수삼(5년근)과 별 차이가 없어 금산 수삼을 사용하였다. 수삼부위 중 주근, 최적온도 $80^{\circ}C$, 최적 추출시간 6시간 추출 시 총당의 추출 효율이 가장 높았다. 또한 총당의 추출효율을 높이기 위해 인삼 추출물에 protease, ${\alpha}-amylase$, cellulase 효소 처리했을 때 추출 효율이 $20\%$까지 높은 것으로 나타났으며, 인삼 추출액에 주정 알콜농도 $70\%$, 동결건조 방법이 총당 회수율이 높았다.

Keywords

References

  1. 천일인쇄사 (1994) 한국인삼연초연구소 : 고려인삼, 대전, 7-27
  2. Brekhman, I.I. (1957) Panax ginseng, Gosudarst Isdat et Med., 2,10-24
  3. Namba, T. (1980) The Encyclopedia of Wakan-Yaku with Color Pictures. Hoikusha, Osaka, Japan, p.23-48
  4. Konno, C., Sugiyama, K., Kano, M., Takahashi, M. and Hikino H. (1984) Isolation and hypoglycemic activity of panaxans A, B, C, D, and E glycans of Panax ginseng roots. Planta Medica, 50, 434 https://doi.org/10.1055/s-2007-969757
  5. Hikino, H., Oshima, Y, Suzuki, Y. and Konno, C. (1985) Isolation and hypoglycemic activity of panaxans F, G, and H glycans of Panax ginseng roots. Kor. J. of Pharm., 39, 331
  6. Gao, Q. P., Kiyohara, H., Jong, J. C. and Yamada, H. (1988) Characterization of anti- complementary acidic heteroglycans from the leaves of Panax ginseng C.A. MEYER. Carbohydrate Research, 181, 175
  7. Konno, C. and Hikino, H. (1987) Isolation and hypoglycemic activity of panaxans M, N, O, and P glycans of Panax ginseng roots. Intl. J. Crude Drug Res , 25, 53
  8. Gao, Q. P., Kiyohara, H., Cyong, J. C. and Yamada, H. (1989) Chemical properties and anti-complementary activities of poly- saccharide fractions from roots and leaves of Panax ginseng. Planta Medica, 55, 9
  9. 윤연숙 (2000) 조혈촉진작용, 골수방어작용, 아세포살해 면역세포 생성작용 및 방사선 민감작용이 우수한 인삼다당체. 특허 2000-49173
  10. Okuda H., Masuno H. and Lee, S. J. (1984) Effect of red ginseng powder on lypolytic and anorexigenic factor (toxohormon L), Proceedings of the 4th International Ginseng Symposium, Korea Ginseng and Tobacco Research Institute, p.145
  11. Okuda, H., Lee, S. D., Matsumra, Y., Zheng, Y., Sekiya, K., Takaku, T., Kameda, K., Hirose, K., Ohtani, K., Tanaka, O. and Sakata, T. (1984) Biological activities of non-saponin compounds isolated from Korean red ginseng, Proceedings of the International Ginseng Symposium, The Society for Korean Ginseng, p.15
  12. Tomodo, M., Shimada, K., Konnoc, C., Sugiyama, K., and Hikino, H (1984) Planta Medica, 50, 436 https://doi.org/10.1055/s-2007-969758
  13. Chung HB, Lee CB, Yoon SW, Lee JH, Choi S, Ko SK, Park H, Lim BO. (2003) Strategies of Globalization for Ginseng Industry. Ijin Publishing, Seoul, Korea, p.10-13
  14. MIller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428 https://doi.org/10.1021/ac60147a030
  15. Han, Y.N., Kim, S.Y., Lee, H.J., Hwang, W.Y. and Han, B.H. (1992) Analysis of Panax ginseng Polysaccharide by Alcian Blue Dye. Meyer and Panax. Korean J. Ginseng Sci., 16, 105-110
  16. Chung, Y.Y., Chung, C.M., Ko, S.R,. and Choi, K.T. (1995) Comparison of Agronomic Characteristics and Chemical Component of Panax ginseng C.A. Meyer and Panax quinquefolium L. Korean J. Ginseng Sci., 19, 160-164
  17. Do JH, Lee HO, Lee SK, Noh KB, (1993) Comparisons of acidic polysaccharide content in various ginseng species and parts. Korean J. Ginseng Sci., 17, 145-147