UV-B-Induced Changes in Carbohydrate Content and Antioxidant Activity in Rice Seedling

  • Sung Jwa-Kyung (National Institute of Agricultural and Science Technology, RDA) ;
  • Lee Su-Yeon (Department of Plant Resources and Science, Hankyong National University) ;
  • Park So-Hyun (Department of Plant Resources and Science, Hankyong National University) ;
  • Jang Byoung-Choon (National Institute of Agricultural and Science Technology, RDA) ;
  • Lee Sang-Min (National Institute of Agricultural and Science Technology, RDA) ;
  • Lee Yong-Hwan (National Institute of Agricultural and Science Technology, RDA) ;
  • Choi Du-Hoi (National Institute of Agricultural and Science Technology, RDA) ;
  • Song Beom-Heon (Department of Agronomy, Chungbuk National University)
  • Published : 2005.06.01

Abstract

The effects of UV-B radiation on the seedling growth, carbohydrate metabolism and antioxidants activities of rice (Oryza sativa L.) were investigated under environmentally controlled chamber. Supplementary UV­B radiation reduced dry matter as well as leaf area, there­fore, relative growth rates (RGR) of seedlings were decreased by up to half compared to control. Photosynthetic products such as soluble sugars and starch were rapidly and significantly reduced by within 1 day of enhanced UV-B radiation due to the inhibition and degradation of photosynthetic processes and thylakoid membrane integrity. In our study, nonstructural carbohydrate levels were proved to be a main indicator on UV-B­induced stress. The behavior of SOD, CAT, APX and POD activities was monitored in the leaves of rice seedlings subjected to UV-B radiation. Under UV-B treatments, SOD activity was initially increased, whereas CAT and POD activities were slowly and slightly increased. However, APX activity showed no presumable results with an increase of UV-B dose. In leaves of rice seedlings, supplementary UV-B radiation caused an increase in free putrescine and spermidine, however spermine remained unaltered, although 24-hrs UV-B treatment slightly increased. This result presumes that an excess UV-B dose may induce ethylene biosynthesis (senescence) rather than polyamine biosynthesis (defense).

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