Effects of Bacillus subtilis Supplementation on Egg Quality, Blood Characteristics and Fecal $NH_3-N$ in Laying Hens

산란계에 Bacillus subtilis의 급여가 계란 품질, 혈액 성상 및 분내 암모니아태 질소 함량에 미치는 영향

  • Kim H. J. (Department ol Animal Resource & Science, Dankook University) ;
  • Woo J. S. (Department ol Animal Resource & Science, Dankook University) ;
  • Kwon O. S. (Department ol Animal Resource & Science, Dankook University) ;
  • Min B. J. (Department ol Animal Resource & Science, Dankook University) ;
  • Shon K. S. (Department ol Animal Resource & Science, Dankook University) ;
  • Jo J. H. (Department ol Animal Resource & Science, Dankook University) ;
  • Chen Y. J. (Department ol Animal Resource & Science, Dankook University) ;
  • Kim I. H. (Department ol Animal Resource & Science, Dankook University)
  • 김해진 (단국대학교 동물자원학과) ;
  • 유종상 (단국대학교 동물자원학과) ;
  • 권오석 (단국대학교 동물자원학과) ;
  • 민병준 (단국대학교 동물자원학과) ;
  • 손경승 (단국대학교 동물자원학과) ;
  • 조진호 (단국대학교 동물자원학과) ;
  • 진영걸 (단국대학교 동물자원학과) ;
  • 김인호 (단국대학교 동물자원학과)
  • Published : 2005.03.01

Abstract

This study was conducted to investigate the effects of Bacillus subtilis on the egg qualify, blood characteristics and fecal $NH_3-N$ in laying hens. A total of two hundred fifty two laying hens were randomly allocated into three treatments with seven replications for six weeks. Dietary treatments included 1) CON (control; basal diet), 2) BS0.2(control + $0.2\%$ Bacillus subtilis) and 3) BS0.4 (control +$0.4\%$ Bacillus subtilis). For overall Period, hen-day egg production tended to increase by the Bacillus subtilis $0.4\%$ in the diets, but was no significant difference. Egg weight, egg shell breaking strength, egg shell thickness, Haugh Unit, yolk color unit and egg yolk index were not affected by treatments. Difference of egg weight and egg shell breaking strength in the BS0.2 treatment tended to increase without significant difference. Difference of egg yolk index in laying hens fed Bacillus subtilis was increased (P<0.05). The concentrations of red blood cell (RBC) and white blood cell (WBC) were not significant difference. $NH_3-N$ concentration in feces with BS0.4 treatment was significantly (P<0.05) lower than control. In conclusion, dietary Bacillus subtilis could decrease fecal $NH_3-N$.

본 연구의 목적은 Bacillus subtilis를 산란계에 급여하였을 때 계란 품질, 혈액 성상 및 분내 암모니아태 질소 함량에 미치는 영향을 조사하기 위하여 실시하였다. 사양시험은 49 주령 Hy-line brown 산란계 252수를 공시하였으며, 처리구로는 옥수수-대두박 기초 사료구(CON; control), 기초 사료구에 Bacillus subtilis를 $\0.2%$ (BS0.2: control + $0.2\%$ Bacillus subtilis)와 $0.4\%$ (BS0.4: control +$0.4\%$ Bacillus subtilis) 첨가한 구로 구성되었다. 총 6주간의 사양시험 기간동안, 산란율은 Bacillus subtilis를 $0.4\%$ 첨가한 처리구가 수적으로 높은 경향을 보였으나 처리구간에 유의적인 차이는 없었다. 난중, 난각 강도, 난각 두께, Haugh Unit, 난황색 그리고 난황계수도 처리구간에 유의적인 차이는 없었다. 그러나 난중과 난각 강도의 증가량은 Bacillus subtilis를 $\0.2%$ 첨가한 처리구가 대조구에 비해 높은 경향을 보였으나 유의적인 차이는 없었으며, 난황계수는 Bacillus subtilis를 첨가한 처리구가 대조구에 비해 유의적으로 증가하였다(P<0.05). RBC와 WBC는 처리구간에 유의적인 차이는 없었다. 분내 암모니아태 질소 함량은 Bacillus subtilis를 $0.4\%$ 첨가한 처리구에서 대조구에 비해 유의적으로 감소하였다(P<0.05). 결론적으로, 산란계 사료내 Bacillus subtilis의 첨가는 분내 암모니아태 질소 함량을 감소시키는 것으로 사료된다.

Keywords

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