Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Antioxidative Activity of the Water Soluble Browning Reaction Products from Korean Red Ginseng

고려홍삼으로부터 분리한 수용성 갈변물질의 항산화 활성

  • Lee, Jong-Won (Ginseng Research Group, KT&G Central Research Institute) ;
  • Park, Chae-Kyu (Ginseng Research Group, KT&G Central Research Institute) ;
  • Do, Jae-Ho (Ginseng Research Group, KT&G Central Research Institute)
  • 이종원 (KT&G 중앙연구원 인삼연구소) ;
  • 박채규 (KT&G 중앙연구원 인삼연구소) ;
  • 도재호 (KT&G 중앙연구원 인삼연구소)
  • Published : 2005.03.01
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Abstract

The purpose of this study was to investigate the biological activities of water soluble browning reaction products(WS-BRPs) isolated from korean red ginseng. Antioxidative activities of WS-BRPs were examined with the various systems. Three different fractions prepared by os moly tic treatment of WS-BRP(fraction L, S-l and S-2) were found to have an ability to donate hydrogen to DPPH and also exhibited the inhibitory activities in lipid peroxidation, consumption of oxygen and protein oxidation of mitochondrial fraction. Especially, L had the strongest activity of these three WS­BRPs in scavenging free radicals. Lipid peroxidation showed the antioxidant effect on linoleic acid oxidation inhibition ratio of $22.5\%,\;31.7\%,\;31.9\%\;and\;33.5\%$, respectivity. And the consumption of oxygen was strongly inhibited by $49.52\%,\;62,44,\;97.54\%$. But three WS-BRPs showed weak inhibitory activity on lipid peroxidation in rat hepatic microsomes.

홍삼에서 분리한 수용성 갈변물질의 항산화 활성을 DPPH 수소공여능 및 미토콘드리아 분획을 이용한 지질과산화, 산소 소모율 및 단백질 산화율을 조사한 결과 다음과 같은 결론을 얻었다. DPPH 수소공여능을 조사한 결과 수용성 갈변물질 S-1, S-2 분획은 반응시간이 경과함에 따라 완만하게 DPPH가 감소하였고, L 분획은 S-1, S-2 분획보다 시간이 경과함에 따라 감소폭이 증가하였다. 미토콘드리아 분획을 이용한 지질과산화 및 산소 소모율의 변화에서 첨가 농도가 높을수록 억제율이 높았으며, 특히, L분획이 S-1 및 S-2 분획보다 높은 활성을 보였다. 또한 수용성 갈변물질을 농도별로 각각 첨가했을시 단백질 산화에서 L분획에서 반응초기에 약간 급속히 진행되는 것으로 보이나, S-1과 S-2 분획에서는 비교적 농도 의존적으로 나타났으며, S-2 분획은 S-1 및 L 분획보다 억제율이 현저히 낮았다.

Keywords

References

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