Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Characteristics of Developmental Stages in Bacterial Biofilm Formation

세균 생물막 형성의 단계별 특징

  • Kim Chang-Beom (Department of Environmental Science, Hankuk University of Foreign Studies) ;
  • Rho Jong-Bok (Department of Environmental Science, Hankuk University of Foreign Studies) ;
  • Lee Hyun-Kyung (Division of Biological Science, Chungbuk National University) ;
  • Choi Sang Ho (Department of Food Science and Technology, Seoul National University) ;
  • Lee Dong-Hun (Division of Biological Science, Chungbuk National University) ;
  • Park Soon-Jung (Department of Parasitology, Yonsei University) ;
  • Lee Kyu-Ho (Department of Environmental Science, Hankuk University of Foreign Studies)
  • 김창범 (한국외국어대학교 환경학과) ;
  • 노종복 (한국외국어대학교 환경학과) ;
  • 이현경 (충북대학교 생명과학부) ;
  • 최상호 (서울대학교 식품공학과) ;
  • 이동훈 (충북대학교 생명과학부) ;
  • 박순정 (연세대학교 의과대학 기생충학교실) ;
  • 이규호 (한국외국어대학교 환경학과)
  • Published : 2005.03.01
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Abstract

Since Anton van Leeuwen­hoek first observed a surface-associated multicellular structure of bacterial cells in the 17th century, it has been shown to exhibit an ability to form a biofilm by numerous bacterial species. The biofilm formation is composed of distinct developmental stages, which include an attachment/adhesion of a single cell, a proliferation toward monolayered coverage, a propagation to aggregated microcolony, a maturation to 3-dimensional structure, and subsequently a local degradation. Investigation to identify the essential factors for bacterial biofilm formation has been performed via classical genetic approaches as well as recently developed technologies. The initial stage requires bacterial motility provided by a flagellum, and outermembrane components for surface signal interaction. Type IV-pilus and autoaggregation factors, e.g., type I-fimbriae or Ag43, are necessary to reach the stages of monolayer and micro colony. The mature biofilm is equipped with extracellular polymeric matrix and internal water-filled channels. This complex architecture can be achieved by differential expressions of several hundred genes, among which the most studied are the genes encoding exopolysaccharide biosyntheses and quorum-sensing regulatory components. The status of our knowledge for the biofilms found in humans and natural ecosystems is discussed in this minireview.

Keywords

References

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